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Coordinating Adaptive and Innate Immunity

协调适应性和先天免疫

基本信息

批准号：
8076588
负责人：
HUA HUANG
金额：
$ 11.13万
依托单位：
NATIONAL JEWISH HEALTH
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-06-28 至 2012-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8076588
关键词：
Alleles Allergens Allergic Disease Allergic inflammation Applications Grants Asthma Basophils Bone Marrow CD4 Positive T Lymphocytes Cell Differentiation process Cells Country Development Disease Effector Cell Fluorescence Genes Goals Helper-Inducer T-Lymphocyte Horns IL4 gene Immune Immunity In Vitro Incidence Infection Inflammation Interleukin-13 Interleukin-4 Interleukin-5 Interleukin-9 Interleukins Lead Lung Measures Mediating Molecular Mus Natural Immunity Parasitic infection Play Pollution Production Proteins Reporter Research Personnel Role Series Severities Site Staging Stem cells Testing Th2 Cells adaptive immunity airway inflammation allergic airway inflammation base cytokine effective therapy eosinophil in vivo insight knock-down mast cell mortality mutant novel programs recombinant virus reconstitution research study response

项目摘要

DESCRIPTION (provided by applicant): Airway inflammation induced by various allergens, pollution, and infections is a major underlying cause of asthma, a disease with alarming increases in incidence and mortality in the western countries in the past two decades. We seek to understand the cellular and molecular basis for exacerbation of asthma. Our immediate goal is to investigate the mechanisms by which CD4+ T helper cells (Th) regulate differentiation of innate Th2-type effector cells. Both CD4+ Th 2 cells and Th2-type innate effector cells have been shown to play critical roles in causing allergic airway inflammation by producing interleukin (IL)-4, IL-5, IL-9, IL-13 and IL-25. However, the mechanisms by which CD4+ Th2 cells regulate innate Th2-type effector cells are unknown. We studied these mechanisms by using IL-4 reporter mice. We found that IL-4 plays an important role in the differentiation of Th2 cytokine-producing innate cells both in vivo and in vitro. Based on our preliminary findings, we hypothesize that IL-4 primes bone marrow progenitor cells into Th2-type innate effector cells via a GATA3-dependent mechanism, while IL-5 does so via STAT5-dependent mechanisms. We propose three specific aims to test our hypothesis. In Aim 1, we will determine whether IL-4 directs bone marrow progenitor cells to differentiate into innate Th2-type effector cells via a GATA3-dependent mechanism; In Aim 2, we will determine whether IL-5 directs bone marrow progenitor cells to differentiate into Th2-type innate effector cells via a STAT5-dependent mechanism; and in Aim 3,we will determine whether CD4+Th2 effector cells coordinate with innate cells to generate Th2-type immunity through secretion of IL-4 and IL-5. These studies will identify novel aspects of interaction between adaptive immunity and innate cells. For diseases such as allergic inflammation, asthma, parasitic infection, and other immune dysregulations, our findings could lead to more effective treatments.

描述（由申请人提供）：由各种过敏原、污染和感染引起的气道炎症是哮喘的主要潜在原因，哮喘是一种在过去二十年中在西方国家发病率和死亡率惊人增加的疾病。我们试图了解哮喘恶化的细胞和分子基础。我们的近期目标是研究CD 4 + T辅助细胞（Th）调节先天性Th 2型效应细胞分化的机制。 CD 4 + Th 2细胞和Th 2型先天性效应细胞通过产生白细胞介素（IL）-4、IL-5、IL-9、IL-13和IL-25在引起过敏性气道炎症中起关键作用。然而，CD 4 + Th 2细胞调节先天性Th 2型效应细胞的机制尚不清楚。我们使用IL-4报告小鼠研究了这些机制。我们发现，IL-4在体内和体外的Th 2细胞的分化中起着重要的作用。基于我们的初步研究结果，我们假设IL-4通过GATA 3依赖性机制将骨髓祖细胞诱导为Th 2型先天性效应细胞，而IL-5通过STAT 5依赖性机制这样做。我们提出了三个具体目标来检验我们的假设。目的1：确定IL-4是否通过GATA 3依赖性机制诱导骨髓祖细胞分化为Th 2型效应细胞;目的2：确定IL-5是否通过STAT 5依赖性机制诱导骨髓祖细胞分化为Th 2型效应细胞;目的3：探讨CD 4 + Th 2效应细胞是否通过分泌IL-4和IL-5与先天性细胞协同产生Th 2型免疫。这些研究将确定适应性免疫和先天细胞之间相互作用的新方面。对于过敏性炎症，哮喘，寄生虫感染和其他免疫失调等疾病，我们的发现可能会导致更有效的治疗方法。

项目成果

期刊论文数量（5）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

IL-4 confers resistance to IL-27-mediated suppression on CD4+ T cells by impairing signal transducer and activator of transcription 1 signaling.

DOI：
10.1016/j.jaci.2013.06.035
发表时间：
2013-10
期刊：
The Journal of allergy and clinical immunology
影响因子：
0
作者：
Chen Z;Wang S;Erekosima N;Li Y;Hong J;Qi X;Merkel P;Nagabhushanam V;Choo E;Katial R;Alam R;Trikha A;Chu HW;Zhuang Y;Jin M;Bai C;Huang H
通讯作者：
Huang H

CCAAT/enhancer-binding protein alpha (C/EBPalpha) is critical for interleukin-4 expression in response to FcepsilonRI receptor cross-linking.

CCAAT/增强子结合蛋白 α (C/EBPα) 对于响应 FcepsilonRI 受体交联的白细胞介素 4 表达至关重要。