ATOPIC DERMATITIS: SKIN AND IMMUNE RESPONSE TO REDUCED ALLERGEN EXPOSURE

特应性皮炎：皮肤和免疫对减少过敏原暴露的反应

• 批准号: 8167150
• 负责人: Judith A Woodfolk
• 金额: $ 0.94万
• 依托单位: UNIVERSITY OF VIRGINIA
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-03-01 至 2013-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8167150
• 关键词: Allergens; Allergic; Antibodies; Atopic Dermatitis; B-Lymphocytes; Cell surface; Clinical; Computer Retrieval of Information on Scientific Projects Database; Data; Discriminant Analysis; Disease; Environment; Felis catus; Flow Cytometry; Funding; Grant; Hospitals; IgE; Immune; Immune response; Immunoglobulin G; In Vitro; Individual; Institution; Lesion; Linear Regressions; Measurement; Monitor; Patients; Peptides; Production; Pyroglyphidae; Regimen; Regression Analysis; Reporting; Research; Research Personnel; Resources; Serum; Skin; Source; Students; T cell response; T-Lymphocyte; T-Lymphocyte Subsets; Testing; Time; United States National Institutes of Health; cytokine

This subproject is one of many research subprojects utilizing the resources provided by a Center grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the Center, which is not necessarily the institution for the investigator. Elevated total IgE and specific IgE to allergens from diverse sources including cat and house dust mite (HDM) have been demonstrated in patients with atopic dermatitis (AD). Improvement of eczematous lesions has been reported when patients are admitted to the hospital, which represents an "allergen-free" environment. Severe clinical disease in AD patients has been attributed to suppression of the immune response or immune dysregulation. We have recently demonstrated that the immune response to allergens is altered at both the B-cell and T-cell level in allergic patients with AD. We will test the hypothesis that allergen avoidance in subjects with IgE ab to common allergens is associated with improvement of skin condition and changes in the immune response to allergens derived from HDM and cat. Serum antibodies (IgG and IgE) and T cell responses to common allergens and allergen-derived peptides will be monitored at weekly intervals (3 weeks maximum) in AD patients who receive a complete allergen avoidance regimen in a hospital environment. Detailed analysis of in vitro T cell responses will include measurement of allergen-stimulated proliferation and cytokine production, expression of cell surface markers by flow cytometry and enumeration of different T cell subsets. Data will be compared at each time point within each individual in order to determine changes which occur during allergen avoidance. Results will also be compared between groups of AD patients with and without IgE ab to common allergens. Statisical tests will include student's t test, cross-validated linear discriminant analysis, and linear regression analysis.

该子项目是利用该技术的众多研究子项目之一 资源由 NIH/NCRR 资助的中心拨款提供。子项目及 研究者 (PI) 可能已从 NIH 的另一个来源获得主要资金， 因此可以在其他 CRISP 条目中表示。列出的机构是 对于中心来说，它不一定是研究者的机构。 特应性皮炎 (AD) 患者的总 IgE 和特异性 IgE 升高，这些过敏原来自不同来源，包括猫和屋尘螨 (HDM)。 据报道，患者入院时湿疹皮损有所改善，这代表了“无过敏原”的环境。 AD 患者的严重临床疾病归因于免疫反应抑制或免疫失调。 我们最近证明，过敏性 AD 患者的 B 细胞和 T 细胞水平对过敏原的免疫反应都发生了改变。 我们将检验以下假设：具有常见过敏原 IgE ab 的受试者回避过敏原与皮肤状况的改善以及对 HDM 和猫过敏原的免疫反应的变化有关。 对于在医院环境中接受完整过敏原避免方案的 AD 患者，将每周（最多 3 周）监测血清抗体（IgG 和 IgE）和 T 细胞对常见过敏原和过敏原衍生肽的反应。 体外 T 细胞反应的详细分析将包括测量过敏原刺激的增殖和细胞因子的产生、通过流式细胞术测量细胞表面标记物的表达以及不同 T 细胞亚群的计数。 将比较每个人每个时间点的数据，以确定避免过敏原期间发生的变化。 还将对具有和不具有常见过敏原 IgE 抗体的 AD 患者组之间的结果进行比较。 统计检验将包括学生 t 检验、交叉验证的线性判别分析和线性回归分析。