Replication Study of Breast Cancer Susceptibility Genes in Blacks

描述(申请人提供)：与所有其他种族/民族相比，非洲裔美国人和非洲人患侵袭性乳腺癌的负担不成比例，原因尚不清楚，也未得到充分研究。缺乏关于非洲裔妇女乳腺癌遗传流行病学的数据阻碍了创新预防措施的发展，以减少健康差距。由于具有潜在大人群归因风险的常见基因变异可能以不同于欧洲血统妇女的方式在非洲血统妇女的乳腺癌发生和进展中发挥作用，我们建议复制之前在全基因组关联研究中确定的5个新的乳腺癌易感基因(涉及11个基因)，在一个具有良好特征的约2200例乳腺癌病例和2300名对照的队列中，全部为非洲血统。这项大型研究的DNA样本和流行病学数据是通过尼日利亚乳腺癌研究(非洲人)、巴巴多斯国家癌症研究(非洲巴巴迪亚人)和芝加哥乳房孢子和乳腺癌家庭登记处(非裔美国人)收集的。我们将使用复制加精细定位策略来对原始研究中确定的完全相同的SNPs和这5个基因组区域中的一组标签SNPs和假定的功能变异进行基因分型。对于非裔美国人，血统信息标记也将进行基因分型，以控制人口分层。多重鉴定程序将被用于鉴定未分型的变异体。将使用逻辑回归来检验相关性并计算奇数比。此外，我们将研究基因与环境的相互作用，因为三个非洲血统的人群在流行病学风险因素方面存在鲜明对比。这项研究将促进对非洲裔妇女乳腺癌遗传风险因素的了解，并将对未来制定新的预防战略具有非常重要的价值。

DESCRIPTION (provided by applicant): African Americans and Africans experience a disproportionate burden of aggressive breast cancer in comparison to all other racial/ethnic groups for reasons that remain unknown and understudied. The paucity of data on the genetic epidemiology of breast cancer for women of African ancestry hinders the development of innovative prevention to reduce health disparities. Because common genetic variants with potential large population attributable risk may play a role in breast cancer development and progression for women of African ancestry in a way different from women of European ancestry, we propose to replicate 5 novel breast cancer susceptibility loci (involving 11 genes) previously identified in genome-wide association studies in a well-characterized cohort of about 2200 breast cancer cases and 2300 controls, all of African ancestry. DNA samples and epidemiologic data for this large study have been collected through Nigerian Breast Cancer Study (Africans), Barbados National Cancer Study (African Barbadians), and the Chicago Breast SPORE and the Breast Cancer Family Registry (African Americans). We will employ the replication plus fine-mapping strategy to genotype exactly same SNPs identified in original studies and a panel of tagSNPs and putative functional variants in these 5 genomic regions. For African Americans, ancestry informative markers will also be genotyped to control for population stratification. Multiple imputation procedure will be used to impute variants that are not genotyped. Logistic regressions will be used to test the associations and calculate odd ratios. Furthermore, we will examine gene-environment interaction as there are sharp contrasts in epidemiologic risk factors among the three populations of African ancestry. This study will advance understanding the genetic risk factors for breast cancer in women of African descent and will be invaluable in developing novel prevention strategies in the future.