Domestic Indoor PM & Childhood Asthma Morbidity

哮喘是儿童中的一种常见疾病。儿童哮喘具有多发性和复杂性 环境暴露，包括可吸入污染物和过敏原。对吸入暴露的反应是 以呼吸道炎症为特征，可能以氧化应激途径激活为特征。在美国， 哮喘的负担是巨大的，对包括年轻人在内的弱势群体的影响不成比例。 儿童、非裔美国人和那些住在市中心的人。这些最脆弱的人通常居住在 室外污染水平过高，以及室内过敏原和 污染物。 我们计划解决室内环境对儿童哮喘的作用这一关键问题，我们 建议对居住在城市社区的儿童进行小组研究，这些社区中有很多非裔美国人和更低的人 收入参与者。这项基于真实世界的研究将补充项目2、3和4的研究结果， 它将类似地检查PM和过敏原的影响，但在使用人类受试者的受控环境中， 动物模型和细胞系统。我们将招募特应性哮喘患者，包括患有和不患有特应性哮喘的患者 对小鼠过敏原致敏，以及正常对照，以解决以下特定目标： 目的1.A：确定室内颗粒物对肺功能和哮喘的日常变化的贡献 对照，在市中心患有特应性哮喘的儿童中。 目的：确定较高的室内PM暴露是否与炎症的生物标志物和 氧化应激。 目的2A：确定小鼠IgE阳性哮喘患者是否有更大的小鼠变应原暴露 与其他特应性哮喘患者(小鼠IgE阴性和/或小鼠变应原低)相比，对PM的易感性 曝光)。 目的2.B：确定高暴露于小鼠变应原的小鼠IgE阳性哮喘(&gt；0.5ug/g 与PM相比，MU m1)对PM的反应具有更高的炎症和氧化应激生物标志物 那些小鼠IgE-E阴性或小鼠过敏原暴露水平较低的人。

Asthma is a common disorder among children. Children with asthma have multiple and complex environmental exposures, including inhalable pollutants and allergens. The response to inhaled exposures is characterized by airway inflammation, and possibly by activation of oxidative stress pathways. In theU.S., the burden of asthma is enormous, with a disproportionate impact on vulnerable groups including young children, African-Americans and those living in the inner city. These most vulnerable people often inhabit an environment that has excessive outdoor pollution levels, as well as high levels of indoor allergens and pollutants. We plan to address the critical questions of the role of the indoor environment on childhood asthma, we propose a panel study of children living in urban neighborhoods enriched with African-American and lower income participants. This real world field-based study will complement the findings from Projects 2, 3 and 4, which will similarly examine the effects of PM and allergen, but in controlled settings using human subjects, animal models and cell systems. We will recruit atopic asthmatics, including those with and without sensitization to mouse allergen, as well as normal controls, to address the following Specific Aims: Aim 1 .A: To determine the contribution of indoor PM to day-to-day variation in lung function and asthma control, among inner city children with atopic asthma. Aim IB: To determine if higher indoor PM exposure is associatedwith biomarkers of inflammation and oxidative stress. Aim 2A: To determine if mouse IgE-positive asthmatics with high mouse allergen exposure have greater susceptibility to PMthan other atopic asthmatics (mouse IgE-negative and/or with low mouse allergen exposure). Aim 2.B: To determine if mouse IgE-positive asthmaticswith high mouse allergen exposure (> 0.5 ug/g of Mus m 1) have elevated biomarkers of inflammation and oxidative stress in response to PM compared to those who are mouse IgE-E negative or who have low mouse allergen exposure..