PROJECT 5

项目5：简单合成模块的可进化性和鲁棒性（Elowitz）（#31-32） 我们研究了噪声在天然和人造细菌转录网络中的作用。我们最初的目标是 探索这些模块的可演化性使我们找到了更精确地分析它们的方法， 噪声影响它们的操作：1）我们进行了原核启动子中顺式调控逻辑的研究， 基于由模块化序列元件组成的组合启动子文库。这项研究发现， 许多启发式规则，用于编程和调节原核生物的组合逻辑行为， 发起人。2)我们分析了导致B中的感受态的瞬时分化系统。枯草杆菌。到 为了了解这种可兴奋开关的运作，我们改变了启动子的强度，电路结构， 随机波动（噪声）。我们确定了分化频率和持续时间的独立调谐 作为设计原则，并表明噪声（波动）实际上是细胞分化所需的37。

Project 5: Evolvability and robustness in simple synthetic modules (Elowitz) (#31-32) We studied the role of noise in natural and man-made bacterial transcriptional networks. Our original aim of exploring the evolvability of these modules led us to methods to analyze them more precisely, by asking how noise affects their operation: 1) We performed a study of the cis-regulatory logic in prokaryotic promoters, based on a combinatorial promoter library composed of modular sequence elements. This study identified a number of heuristic rules for programming and modulating the combinatorial logic behavior of prokaryotic promoters. 2) We analyzed transient differentiation system that leads to competence in B. subtilis. To understand the operation of this excitable switch, we varied promoter strengths, circuit architecture, and stochastic fluctuations (noise). We identified the independent tuning of differentiation frequency and duration as a design principle, and showed that noise (fluctuations) is actually required for cellular differentiation37.