PROJECT 5

项目5：简单合成模块的可进化性和健壮性(Elowitz)(#31-32) 我们研究了噪声在自然和人造细菌转录网络中的作用。我们的初衷是 探索这些模块的可进化性使我们找到了更准确地分析它们的方法，通过询问 噪声影响它们的运作：1)我们对原核生物启动子中的顺式调控逻辑进行了研究， 基于由模块序列元件组成的组合启动子文库。这项研究发现了一个 编程和调节原核生物组合逻辑行为的启发式规则的数量 推动者。2)分析了导致枯草芽孢杆菌竞争能力的瞬时分化系统。至 为了了解这种可激发开关的工作原理，我们改变了启动器的强度、电路结构和 随机波动(噪声)。我们确定了分化频率和持续时间的独立调谐 作为一项设计原则，并表明噪音(波动)实际上是细胞分化所必需的37。

Project 5: Evolvability and robustness in simple synthetic modules (Elowitz) (#31-32) We studied the role of noise in natural and man-made bacterial transcriptional networks. Our original aim of exploring the evolvability of these modules led us to methods to analyze them more precisely, by asking how noise affects their operation: 1) We performed a study of the cis-regulatory logic in prokaryotic promoters, based on a combinatorial promoter library composed of modular sequence elements. This study identified a number of heuristic rules for programming and modulating the combinatorial logic behavior of prokaryotic promoters. 2) We analyzed transient differentiation system that leads to competence in B. subtilis. To understand the operation of this excitable switch, we varied promoter strengths, circuit architecture, and stochastic fluctuations (noise). We identified the independent tuning of differentiation frequency and duration as a design principle, and showed that noise (fluctuations) is actually required for cellular differentiation37.