The Genetic Architecture of Aggressive Behavior

攻击行为的遗传结构

• 批准号: 6935104
• 负责人: ALEXIS C EDWARDS
• 金额: $ 3.01万
• 依托单位: NORTH CAROLINA STATE UNIVERSITY RALEIGH
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6935104
• 关键词: Drosophilidae; aggression; behavioral /social science research tag; behavioral genetics; ethology; gene environment interaction; gene expression; gene expression profiling; gene interaction; gene mutation; genetic manipulation; genetic mapping; genetic markers; male; predoctoral investigator; quantitative trait loci

DESCRIPTION (provided by applicant): Aggressive behavior is a quantitative trait, affected by multiple interacting genes with individually small effects, whose expression is sensitive to the environment. I propose several complementary approaches to investigate the genetic basis of aggressive behavior in Drosophila males, using an assay that I developed to rapidly and reproducibly quantify aggressive behavior. Specifically, I will: (1) Map quantitative trait loci (QTLs) affecting variation in aggression. I have localized one QTL with a large effect and will use complementation tests to deficiencies and mutations of positional candidate genes to identify candidate gene(s) corresponding to the QTL. (2) Use artificial selection to derive lines with increased and decreased levels of aggression. This experiment is currently at generation 7.1 will assess whether other traits have evolved as correlated responses to selection for aggression, and use whole genome expression profiling to identify candidate genes whose expression differs significantly between the selection lines. (3) Identify new candidate genes regulating aggressive behavior by quantifying the aggressive behavior of a collection of single P-element insertion lines in a homozygous genetic background.

描述（由申请人提供）：攻击性行为是一种数量性状，受多个相互作用的基因影响，单个影响很小，其表达对环境敏感。我提出了几种互补的方法来研究雄性果蝇攻击行为的遗传基础，使用我开发的一种快速可重复量化攻击行为的测定方法。具体而言，我将：(1)绘制影响攻击变异的数量性状位点（qtl）。我已经定位了一个具有较大效应的QTL，并将对位置候选基因的缺陷和突变进行互补测试，以确定与该QTL对应的候选基因。(2)采用人工选择的方法，获得攻击性增强和减弱的品系。该实验目前在第7.1代，将评估其他性状是否已经进化为对攻击选择的相关反应，并使用全基因组表达谱来识别在选择系之间表达差异显著的候选基因。(3)通过对纯合子遗传背景下的单p元件插入系的攻击行为进行量化，确定新的候选基因调控攻击行为。