Role of Innate and Adaptive Immune Systems in Drug-Induced Liver Disease

在这份报告中，我们比较了适应性免疫反应氟烷治疗后的正常雌性Balb/cJ小鼠的Balb/cJ小鼠耗尽的调节性T细胞。与正常Balb/cJ小鼠相比，当在培养物中用TFA-蛋白加合物处理时，耗尽调节性T细胞的小鼠具有显著更高的抗TFA-蛋白抗体血清水平和升高的T细胞增殖。此外，有一个显着的浸润TGF-β-产生调节性T细胞的正常Balb/cJ小鼠氟烷治疗后的肝脏。这些结果表明，调节性T细胞可能具有重要的抑制作用的适应性免疫系统对蛋白质加合物的药物释放从受损的肝细胞和免疫病理学对肝脏的反应。 结论：这些初步发现为进一步研究调节性T细胞在DILD发病机制和易感性中的作用提供了依据。

In this report, we compared the adaptive immune responses following halothane treatment of normal female Balb/cJ mice to Balb/cJ mice depleted of regulatory T cells. Mice depleted of regulatory T cells had significantly higher serum levels of anti-TFA-protein antibodies and elevated T cell proliferation when treated in culture with TFA-protein adducts as compared to normal Balb/cJ mice. Moreover, there was a significant infiltration of TGF-beta-producing T regulatory cells into the liver of normal Balb/cJ mice following halothane treatment. These results suggest that T regulatory cells may have an important inhibitory effect on the adaptive immune system reaction against protein adducts of drugs released from damaged hepatocytes and immunopathology against the liver. Conclusion: These preliminary findings warrant further studies into the role of regulatory T cells in the mechanisms and susceptibility to DILD.