Protection of the Heart by PD-1 and PD-L1

PD-1 和 PD-L1 保护心脏

• 批准号: 8612207
• 负责人: ANDREW H LICHTMAN
• 金额: $ 44.26万
• 依托单位: BRIGHAM AND WOMEN'S HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-02-17 至 2018-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8612207
• 关键词: Address; Affect; Antigens; Attention; Autoantigens; Autoimmune Process; CD28 gene; CD4 Positive T Lymphocytes; CD8B1 gene; Cardiac; Cardiomyopathies; Cell surface; Cells; Chronic; Clinical; Clinical Research; Cytoprotection; Data; Dendritic Cells; Development; Dilated Cardiomyopathy; Disease; Endothelial Cells; Ensure; Family; Fibrosis; Gene Expression; Goals; Graft Rejection; Heart; Heart Diseases; Heart Transplantation; Heart failure; Immune; Immune response; Immunotherapy; Infection; Inflammation; Inflammation Mediators; Inflammatory; Inflammatory Response; Injury; Knowledge; Ligands; Lymphocyte; Malignant Neoplasms; Mediating; Methods; Modality; Modeling; Molecular; Mus; Muscle Cells; Myelogenous; Myeloid Cells; Myocardial; Myocardial Infarction; Myocardial Reperfusion Injury; Myocarditis; Myocardium; Pathogenesis; Pathway interactions; Phenotype; Play; Predisposition; Regulation; Regulatory T-Lymphocyte; Risk; Role; Signal Transduction; Sirolimus; T cell regulation; T cell response; T-Lymphocyte; Testing; Therapeutic; Time; Viral; Virus Diseases; Work; allograft rejection; cancer risk; cell type; cytokine therapy; design; gene function; heart allograft; heart function; insight; macrophage; microbial; monocyte; prevent; public health relevance; regenerative; response; therapeutic development

DESCRIPTION (provided by applicant): Myocardial inflammation occurs in in response to ischemic injury, infections, and activation of autoreactive or alloreactive lymphocytes. Myocardial function is impaired by inflammatory mediators, and inflammation contributes to chronic myocardial injury, fibrosis, and heart failure. Innate and adaptive immune responses are tightly regulated in the heart, which serves to prevent maladaptive inflammation. Nonetheless, these mechanisms are poorly understood, and when they fail, inflammatory responses are central to the pathogenesis of myocardial disease. A better understanding of the normal mechanisms that suppress myocardial inflammation is key to the design of therapeutic strategies to limit inflammatory damage in the setting of several cardiac diseases. Extensive preliminary work has demonstrated that the molecules PD-1 and its ligand PD-L1 are important regulators of adaptive immune responses to microbial, alloreactive and self- antigens. In particular, the PD 1/PD L1 pathway appears to be a central negative regulatory checkpoint in controlling immune responses in the heart. However, because of the complexities of the cellular expression of PD-1 and PD-L1, it is still unclear how the pathway is actually engaged to suppress myocardial inflammation. The broad objectives of this project are to discover how cardiac inflammation is regulated by endothelial, myocyte, and dendritic cell PD-L1, and by myeloid PD-1. The work will take advantage of newly developed mouse lines with lineage specific deficiencies of PD-L1 and PD-1, which will be used in the context of models of innate and adaptive immune mediated myocardial inflammation. These approaches will be organized into the following three Specific Aims: Aim 1. Determine which cell types mediate PD-L1-dependent protection against T cells in the heart, the mechanisms of protection, and the feasibility of therapeutically engaging these mechanisms. Aim 2. Test the hypothesis that the PD-1-PD-L1 pathway directly regulates myeloid cell inflammatory responses in the heart. Aim 3. Develop PD-1:PD-L1 dependent methods to therapeutically induced T cell tolerance to myocardial antigens The data generated by the completion of these Aims will define for the first time the regulation of cardiac inflammation by endothelial, myocyte, and DC PD-L1, and by myeloid PD-1. The findings will be important for development of therapeutic modalities to treat myocardial inflammation and for an understanding of the risks of therapeutic approaches to block PD-1 and PD-L1, which are actively being developed in cancer and chronic viral infection.

描述(由申请人提供)：心肌炎症发生在对缺血损伤、感染和自身反应或异体反应淋巴细胞的激活的反应中。炎症介质会损害心肌功能，炎症会导致慢性心肌损伤、纤维化和心力衰竭。先天性和获得性免疫反应在心脏中受到严格调控，这有助于防止适应性不良的炎症。然而，人们对这些机制知之甚少，当它们失败时，炎症反应是心肌疾病发病机制的核心。更好地了解抑制心肌炎症的正常机制是设计治疗策略的关键，以限制几种心脏疾病的炎症损害。大量的前期工作表明，PD-1及其配体PD-L1是对微生物、同种异体反应和自身抗原的适应性免疫反应的重要调节因子。特别是，PD 1/PD L1通路似乎是控制心脏免疫反应的中央负调控检查点。然而，由于PD-1和PD-L1在细胞中表达的复杂性，目前仍不清楚该通路是如何参与抑制心肌炎症的。这个项目的广泛目标是发现心脏炎症是如何由内皮细胞、心肌细胞和树突状细胞PD-L1以及髓系PD-1调节的。这项工作将利用新开发的具有PD-L1和PD-1谱系特异性缺陷的小鼠品系，这些品系将用于先天性和获得性免疫介导的心肌炎症模型。这些方法将被组织为以下三个具体目标：目的1.确定哪些细胞类型介导了心脏对PD-L1依赖的T细胞的保护，保护机制，以及在治疗上使用这些机制的可行性。目的2.验证PD-1-PD-L1通路直接调节心脏髓系细胞炎症反应的假说。目的3.发展PD-1：PD-L1依赖的方法，以治疗性地诱导T细胞对心肌抗原的耐受。完成这些目标所产生的数据将首次定义内皮细胞、心肌细胞、DC PD-L1和髓系PD-1对心脏炎症的调节。这些发现对于开发治疗心肌炎的治疗方法以及了解阻断PD-1和PD-L1的治疗方法的风险将是重要的，这两种治疗方法正在积极地开发用于癌症和慢性病毒感染。