The molecular basis of planarian regeneration

项目负责人/主要研究者（最后，第一，中间）：Sanchez阿尔瓦拉多，Alejandro 项目随附资料（见说明）： 该项目的首要目标是为动物再生过程定义一个机械基础。 该项目利用了上一次供资期间取得的方法上的进展和结果。 阶段：1）定义高时间分辨率、全基因组的再生表达谱; 2） 询问已知的胚胎信号通路在组织再生的成人环境中的功能 和稳态，并进行一个正式的比较再生机制相比， 胚胎发生; 3）揭示截肢后成人器官再生相关的基因;以及4） 启动再生的比较研究，以测试我们发现的普遍性。所有三条线 调查工作是相互配合，它们的结合应该为我们提供一套高分辨率的 调节再生和再生能力的分子过程。到目前为止，这种方法已经使我们 发现新的动物细胞生物学和已知基因在成人背景下的功能，并定义功能 对于许多保守的动物基因，其功能仍然未知。鉴于高度的 存在于真涡虫和脊椎动物之间的进化保守性， 在涡虫中的功能将促进对人类干细胞功能、再生和创伤的研究 治愈，有效地推进人类健康的前沿。 相关性（参见说明）： 该项目的首要目标是为动物再生过程定义一个机械基础。 鉴于存在于涡虫和脊椎动物之间的高度进化保守性， 对真涡虫基因功能的表征将促进人类干细胞功能的研究， 再生和伤口愈合，有效地推进这些人类健康的前沿。

Program Director/Principal Investigator (Last, First, Middle): Sanchez AlvaradO, Alejandro PROJECT SUIVIMARY (See instructions): The overarching goal of this project is to define a mechanistic basis for the process of animal regeneration. This project takes advantage of methodological advances and findings obtained during the last funding period to: 1) define a high temporal resolution, genome-wide, expression profile of regeneration; 2) interrogate the functions of known embryonic signaling pathways in the adult contexts of tissue regeneration and homeostasis, and to carry out a formal comparison of how the mechanisms of regeneration compare to embryogenesis; 3) uncover genes involved in the regeneration of adult organs after amputation; and 4) Initiate comparative studies of regeneration to test the universality of our findings. All three lines of investigation synergize with each other and their integration should provide us with a high-resolution set of molecular processes regulating regeneration and regenerative capacities. Thus far, this approach has led us to uncover novel animal cell biology and functions in adult contexts of known genes, and to define functions for the many conserved animal genes for which functions are still unknown. Given the high degree of evolutionary conservation that exits between planarians and vertebrates, the characterization of gene functions in planarians will advance efforts to study human stem-cell function, regeneration and wound healing, effectively advancing these frontiers of human health. RELEVANCE (See instructions): The overarching goal of this project is to define a mechanistic basis for the process of animal regeneration. Given the high degree of evolutionary conservation that exits between planarians and vertebrates, the characterization of gene functions in planarians will advance efforts to study human stem-cell function, regeneration and wound healing, effectively advancing these frontiers of human health.