The molecular basis of planarian regeneration

项目主任/首席调查员(最后、第一、中间)：桑切斯·阿尔瓦拉多，亚历杭德罗 项目SuIVIMARY(请参阅说明)： 这个项目的首要目标是为动物再生过程定义一个机械基础。 该项目利用了上次资助期间取得的方法学进步和研究成果。 周期至：1)定义高时间分辨率、全基因组范围的再生表达谱；2) 已知的胚胎信号通路在成人组织再生环境中的功能 和动态平衡，并对再生机制与 胚胎发生；3)发现参与成年器官截肢后再生的基因；以及4) 启动再生的比较研究，以检验我们发现的普遍性。所有三条线路 调查相互协同，它们的整合应该为我们提供一套高分辨率的 调节再生和再生能力的分子过程。到目前为止，这种方法使我们 揭示已知基因在成人背景下的新的动物细胞生物学和功能，并定义功能 对于许多保守的动物基因，其功能尚不清楚。鉴于……的高度 在浮游生物和脊椎动物之间存在的进化守恒，基因的特征 行星动物的功能将推动对人类干细胞功能、再生和创伤的研究 治愈，有效地推进人类健康的这些前沿领域。 相关性(请参阅说明)： 这个项目的首要目标是为动物再生过程定义一个机械基础。 鉴于浮游生物和脊椎动物之间存在着高度的进化保守， 对行星动物基因功能的表征将推动研究人类干细胞功能的努力， 再生和伤口愈合，有效地促进了人类健康的这些前沿领域。

Program Director/Principal Investigator (Last, First, Middle): Sanchez AlvaradO, Alejandro PROJECT SUIVIMARY (See instructions): The overarching goal of this project is to define a mechanistic basis for the process of animal regeneration. This project takes advantage of methodological advances and findings obtained during the last funding period to: 1) define a high temporal resolution, genome-wide, expression profile of regeneration; 2) interrogate the functions of known embryonic signaling pathways in the adult contexts of tissue regeneration and homeostasis, and to carry out a formal comparison of how the mechanisms of regeneration compare to embryogenesis; 3) uncover genes involved in the regeneration of adult organs after amputation; and 4) Initiate comparative studies of regeneration to test the universality of our findings. All three lines of investigation synergize with each other and their integration should provide us with a high-resolution set of molecular processes regulating regeneration and regenerative capacities. Thus far, this approach has led us to uncover novel animal cell biology and functions in adult contexts of known genes, and to define functions for the many conserved animal genes for which functions are still unknown. Given the high degree of evolutionary conservation that exits between planarians and vertebrates, the characterization of gene functions in planarians will advance efforts to study human stem-cell function, regeneration and wound healing, effectively advancing these frontiers of human health. RELEVANCE (See instructions): The overarching goal of this project is to define a mechanistic basis for the process of animal regeneration. Given the high degree of evolutionary conservation that exits between planarians and vertebrates, the characterization of gene functions in planarians will advance efforts to study human stem-cell function, regeneration and wound healing, effectively advancing these frontiers of human health.