Acquisition of a Hybrid Quadrupole Time of Flight LC-MS/MS System for the Cornell

描述（由申请人提供）：新的质谱（MS）技术为具有突破性发现潜力的创造性应用提供了绝佳的机会。我们申请资助一个高性能Q-TOF质谱仪（AB SCIEX TripleTOF”5600+），与超高效液相色谱系统（基于芯片的ekspert”nanoLC 425）接口。这个最先进的系统将位于康奈尔大学生物技术资源中心（BRC）的蛋白质组学和质谱设施。在BRC中实施该仪器作为全校共享的研究资源，将使NIH资助的广泛研究能够以成本效益的方式获得这一重要技术。康奈尔大学对蛋白质组学和质谱服务的需求不断增长，过去九年来该设施用户和样品处理数量的快速持续增长就表明了这一点。拟议的综合仪器系统将扩大该设施的容量和能力，但不会重复核心设施的现有仪器，目前该设施已接近满负荷使用。对于涉及复杂样品分析的应用而言，提高性能和容量的需求是特别关键的，所述复杂样品分析需要增强的扫描速度、高灵敏度和分辨率以及高度精确的质量测量。所要求的集成系统将提供改进的动态范围，并将使低丰度蛋白质和复杂样品中的翻译后修饰（PTM）的肽的可靠鉴定成为可能。TripleTOF 5600+将显著增强该设施的能力，以支持定性探索中的蛋白质组学发现研究以及涉及复杂生物样品的高通量分析和高分辨率定量研究。配备cHiPLC（R）系统的ekspert”nanoLC 425是一种集成芯片UHPLC，可提供广泛的二维LC灵活性和连续的直接纳米流动。它在使用基于芯片的色谱柱的工作流程选择方面具有灵活性，并为全局发现和更高通量的靶向定量提供了广泛的流速范围。nanoLC 425系统将在MS之前提供自动化样品处理和高分辨率肽分离，这将使非常复杂的样品能够连续24/7分析。BRC在运行LC MS仪器系统和提供MS应用程序作为核心设施服务方面拥有丰富的经验。拟议的仪器系统将很容易集成到该设施的既定蛋白质组学工作流程，并将显着扩大该设施的定量蛋白质组学管道。将仪器系统放置在BRC中将使NIH资助的各种研究人员能够开展否则在经济或技术上不可行的研究项目。该提案描述了来自16个NIH资助的研究小组的39个项目，这些项目将使用拟议的系统。所要求的工具将有利于广泛的当前和未来NIH资助的项目。

DESCRIPTION (provided by applicant): New mass spectrometry (MS) technologies present an exceptional opportunity for creative applications with potential for breakthrough discoveries. We request funding a high performance Q-TOF mass spectrometer (AB SCIEX TripleTOF" 5600+), interfaced with an ultra-high performance liquid chromatography system (chip-based ekspert" nanoLC 425). This state-of-the-art system will be located in the Proteomics and Mass Spectrometry Facility of the Cornell University Biotechnology Resource Center (BRC). Implementation of the instrument in the BRC as a university-wide shared research resource will enable cost effective access to this vital technology for a broad range of NIH-funded research. There is a growing demand for proteomics and mass spectrometry services at Cornell, as shown by the rapid and continuing growth in the number of facility users and samples processed by the facility over the past nine years. The proposed integrated instrument system will expand the capacity and capabilities of the facility but not duplicate the existing instrumentation at the core facility, which is currently utilized at near full capacity. The need fr increased performance and capacity is particularly critical with respect to applications involving analysis of complex samples which require enhanced scan speed, high sensitivity and resolution, and highly accurate mass measurements. The integrated system requested will provide improved dynamic range and will enable confident identification of peptides from low abundance proteins and of post-translational modifications (PTMs) in complex samples. The TripleTOF 5600+ will significantly enhance the facility's capability to support proteomics discovery studies in qualitative exploration as well as high throughput profiling and high-resolution quantification studies involving complex biological samples. The ekspert" nanoLC 425 with the cHiPLC(R) system is an integrated chip-based UHPLC that provides broad 2D LC flexibility and continuous direct nano flow. It has flexibility in workflow selection using chip-baed columns and offers a broad range of flow rates for both global discovery and higher throughput targeted quantitation. The nanoLC 425 system will provide automated sample handing and high resolution peptide separation prior to MS, which will enable continuous 24/7 analysis of very complex samples. The BRC has extensive experience in running LC MS instrument systems and providing MS applications as core facility services. The proposed instrument system will be readily integrated into the facility's established proteomics workflows and will significantly expand the facility's quantitative proteomics pipeline. Placement of the instrument system in the BRC will enable a diverse group of NIH funded investigators to undertake research projects that would not otherwise be economically or technically feasible. This proposal describes 39 projects from 16 NIH funded investigator groups that will utilize the proposed system. The requested instrument will benefit a wide range of current and future NIH-funded projects.