Acquisition of a Hybrid Quadrupole Time of Flight LC-MS/MS System for the Cornell

描述（由申请人提供）：新的质谱（MS）技术为创造性应用提供了一个特殊的机会，具有突破性发现的潜力。我们要求资助一台高性能Q-TOF质谱仪（AB SCIEX TripleTOF“5600+”），与超高性能液相色谱系统（基于芯片的ekspert“nanoLC 425）接口。这个最先进的系统将位于康奈尔大学生物技术资源中心（BRC）的蛋白质组学和质谱分析设施。在BRC中实施该仪器作为全校范围的共享研究资源，将使nih资助的广泛研究能够以经济有效的方式获得这一重要技术。康奈尔大学对蛋白质组学和质谱分析服务的需求不断增长，在过去的九年里，设备用户数量和设备处理的样品数量迅速持续增长。拟议的综合仪器系统将扩大该设施的容量和能力，但不会重复核心设施的现有仪器，核心设施目前已接近满负荷使用。对于涉及复杂样品分析的应用，需要提高扫描速度，高灵敏度和分辨率以及高度精确的质量测量，对提高性能和容量的需求尤为关键。所要求的集成系统将提供改进的动态范围，并将能够从低丰度蛋白质和复杂样品的翻译后修饰（PTMs）中自信地识别肽。TripleTOF 5600+将显著增强该设施的能力，支持蛋白质组学发现研究的定性探索，以及涉及复杂生物样品的高通量分析和高分辨率定量研究。带有cHiPLC(R)系统的ekspert“nanoLC 425是一种集成芯片的UHPLC，可提供广泛的2D LC灵活性和连续的直接纳米流动。它在使用基于芯片的色谱柱的工作流程选择方面具有灵活性，并为全球发现和更高吞吐量的目标定量提供了广泛的流量范围。nanoLC 425系统将在质谱之前提供自动样品处理和高分辨率肽分离，这将能够连续24/7分析非常复杂的样品。BRC在运行LC质谱仪器系统和提供MS应用程序作为核心设施服务方面拥有丰富的经验。拟议的仪器系统将很容易集成到该设施已建立的蛋白质组学工作流程中，并将大大扩展该设施的定量蛋白质组学管道。在BRC中放置仪器系统将使NIH资助的不同研究人员群体能够开展在经济或技术上不可行的研究项目。该提案描述了来自16个NIH资助的研究小组的39个项目，这些项目将利用拟议的系统。所要求的仪器将有利于广泛的当前和未来美国国立卫生研究院资助的项目。

DESCRIPTION (provided by applicant): New mass spectrometry (MS) technologies present an exceptional opportunity for creative applications with potential for breakthrough discoveries. We request funding a high performance Q-TOF mass spectrometer (AB SCIEX TripleTOF" 5600+), interfaced with an ultra-high performance liquid chromatography system (chip-based ekspert" nanoLC 425). This state-of-the-art system will be located in the Proteomics and Mass Spectrometry Facility of the Cornell University Biotechnology Resource Center (BRC). Implementation of the instrument in the BRC as a university-wide shared research resource will enable cost effective access to this vital technology for a broad range of NIH-funded research. There is a growing demand for proteomics and mass spectrometry services at Cornell, as shown by the rapid and continuing growth in the number of facility users and samples processed by the facility over the past nine years. The proposed integrated instrument system will expand the capacity and capabilities of the facility but not duplicate the existing instrumentation at the core facility, which is currently utilized at near full capacity. The need fr increased performance and capacity is particularly critical with respect to applications involving analysis of complex samples which require enhanced scan speed, high sensitivity and resolution, and highly accurate mass measurements. The integrated system requested will provide improved dynamic range and will enable confident identification of peptides from low abundance proteins and of post-translational modifications (PTMs) in complex samples. The TripleTOF 5600+ will significantly enhance the facility's capability to support proteomics discovery studies in qualitative exploration as well as high throughput profiling and high-resolution quantification studies involving complex biological samples. The ekspert" nanoLC 425 with the cHiPLC(R) system is an integrated chip-based UHPLC that provides broad 2D LC flexibility and continuous direct nano flow. It has flexibility in workflow selection using chip-baed columns and offers a broad range of flow rates for both global discovery and higher throughput targeted quantitation. The nanoLC 425 system will provide automated sample handing and high resolution peptide separation prior to MS, which will enable continuous 24/7 analysis of very complex samples. The BRC has extensive experience in running LC MS instrument systems and providing MS applications as core facility services. The proposed instrument system will be readily integrated into the facility's established proteomics workflows and will significantly expand the facility's quantitative proteomics pipeline. Placement of the instrument system in the BRC will enable a diverse group of NIH funded investigators to undertake research projects that would not otherwise be economically or technically feasible. This proposal describes 39 projects from 16 NIH funded investigator groups that will utilize the proposed system. The requested instrument will benefit a wide range of current and future NIH-funded projects.