Fiber Scanning Stimulated Raman Scattering (SRS) Endoscope

光纤扫描受激拉曼散射 (SRS) 内窥镜

• 批准号: 8831433
• 负责人: Christian Freudiger
• 金额: $ 22.5万
• 依托单位: INVENIO IMAGING INC.
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-18 至 2016-02-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8831433
• 关键词: Address; Adoption; Animal Model; Appearance; Area; Arizona; Biocompatible; Biological; Brain; Brain Neoplasms; Chemicals; Clinical; Collaborations; Cues; Custom; Data; Detection; Development; Devices; Diagnostic Imaging; Dyes; Employee Strikes; Endoscopes; Endoscopy; Engineering; Excision; Fiber; Glioblastoma; Glioma; Goals; Gold; Hand; Hematoxylin and Eosin Staining Method; Histology; Histopathology; Human; Image; In Situ; Label; Laboratory Research; Lasers; Left; Lighting; Magnetic Resonance Imaging; Malignant neoplasm of brain; Maps; Michigan; Microscope; Microscopic; Microscopy; Molecular; Nature; Nuclear; Oncologist; Operating Rooms; Operative Surgical Procedures; Optics; Patients; Phase; Physiologic pulse; Polystyrenes; Postoperative Period; Procedures; Process; Protocols documentation; RNA Splicing; Reading; Recurrence; Research; Resected; Residual Tumors; Resolution; Scanning; Science; Simulate; Source; Specimen; Spectrum Analysis; Speed; Staining method; Stains; Surface; Surgically-Created Resection Cavity; System; Techniques; Technology; Time; Tissues; Tumor Tissue; Universities; Validation; Visual; Washington; Work; base; blind; cancer surgery; cost; design; detector; improved; in vivo; lens; meetings; miniaturize; mouse model; multidisciplinary; novel; photonics; public health relevance; time use; translational medicine; tumor

DESCRIPTION (provided by applicant): Achieving maximal tumor removal with minimal collateral damage to healthy tissue is a challenge in tumor surgery, because there are no reliable visual cues to indicate completeness of resection. In contrast, on a microscopic level, the differences between tumor and non-infiltrated tissues are clear. However, traditional histopathologic techniques require that the tissue be removed from the operative field, processed, and stained. Therefore, histopathology can neither be performed in situ nor in real-time. Suboptimal surgical results are extremely common in brain cancer surgery (64,000 US cases/year). Tumor that could have been safely removed has been identified in the operative cavity in 76% of cases, by post-operative MRI. In most cases, tumor recurrence occurs near the resection cavity. An in vivo technique for real-time imaging of the resection margins at the time of surgery would be highly desirable. Stimulated Raman Scattering (SRS) microscopy combines the advantages of morphological and chemical imaging of traditional histology but without requiring tissue removal or dye staining. It can thus be used in situ and in real- time. Mapping of a 2x2cm2 area can be achieved in approximately 2.5min and has the potential to provide quantitative clinical endpoints for brain tumor surgery. The accuracy of tumor detection in an animal model of glioblastoma with a state-of-the-art research-type SRS microscope has been shown to be comparable to traditional H&E microscopy, without requiring tissue removal or staining. However, there is no system that meets all the requirements for clinical SRS imaging. The present proposal is to develop a handheld SRS endoscope that is suited for use in a surgical cavity and achieves diagnostically relevant image quality and imaging speed. The goal of Phase 1 is to obtain proof-of-concept by leveraging established technologies, to the largest degree possible. None of the components have been designed for the purpose of SRS endoscopy but should be able to be modified to work for that purpose, with predictable limitations. If Phase 1 proof-of-concept is achieved, custom components would be designed in Phase 2 to improve image quality and speed. The PI has assembled a multidisciplinary team of academic, industrial and clinical thought leaders for laser engineering, miniaturized scanner development, optical design, and clinical validation. The specific aims are to (1) develop an all-fiber scanning fiber SRS endoscope and (2) rigorously demonstrate that the SRS endoscope produces diagnostically relevant image quality.

描述(申请人提供)：实现最大限度的肿瘤切除，同时对健康组织造成最小的附带损害是肿瘤外科中的一个挑战，因为没有可靠的视觉线索来指示切除的完整性。相比之下，在显微镜水平上，肿瘤和非浸润性组织之间的差异是明显的。然而，传统的组织病理学技术要求将组织从手术野中取出，进行处理和染色。因此，组织病理学既不能在原位进行，也不能实时进行。次佳的手术结果在脑癌手术中非常常见(每年6.4万例美国病例)。通过术后核磁共振检查，76%的病例在手术腔内发现了本可以安全切除的肿瘤。在大多数情况下，肿瘤复发发生在切除腔附近。在手术时对切除边缘进行实时成像的体内技术将是非常可取的。受激拉曼散射(SRS)显微镜结合了传统组织学的形态成像和化学成像的优点，而不需要去除组织或进行染料染色。因此，它可以在现场和实时使用。映射 2x2cm2的面积可以在大约2.5分钟内达到，并有可能为脑肿瘤手术提供定量的临床终点。使用最先进的研究型SRS显微镜在胶质母细胞瘤动物模型中检测肿瘤的准确性已被证明与传统的H&E显微镜相当，不需要切除组织或染色。然而，目前还没有一种系统能够满足临床SRS成像的所有要求。目前的建议是开发一种手持式SRS内窥镜，该内窥镜适合在外科手术腔中使用，并实现与诊断相关的图像质量和成像速度。阶段1的目标是通过最大程度地利用已建立的技术来获得概念验证。这些部件都不是为SRS内窥镜检查目的而设计的，但应该能够进行修改以用于该目的，并具有可预见的局限性。如果完成了第一阶段的概念验证，则将在第二阶段设计定制组件，以提高图像质量和速度。PI已经组建了一支由学术、工业和临床思想领袖组成的多学科团队，负责激光工程、微型扫描仪开发、光学设计和临床验证。其具体目标是(1)开发全光纤扫描光纤SRS内窥镜和(2)严格证明SRS内窥镜产生与诊断相关的图像质量。