Using a disease-affected cell to synthesize its own drug

使用受疾病影响的细胞合成自己的药物

• 批准号: 8948649
• 负责人: Matthew D Disney
• 金额: $ 96万
• 依托单位: SCRIPPS FLORIDA
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-09-30 至 2020-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8948649
• 关键词: Affect; Animals; Binding; Biology; Biomedical Research; Blood - brain barrier anatomy; Brain; Cells; Chemicals; Code; Custom; Data; Disease; Fragile X Syndrome; Genes; Human; Huntington Disease; Image; Lead; Life; Medicine; Microsatellite Repeats; Modality; Molecular Weight; Muscular Dystrophies; Mutation; Pathology; Patients; Pharmaceutical Preparations; Precision therapeutics; Proteins; RNA; Reaction; Technology; Therapeutic; Tissues; Toxic effect; Transcript; Untranslated RNA; Weight Gain; catalyst; genome sequencing; human disease; inhibitor/antagonist; innovation; nervous system disorder; precision medicine

 DESCRIPTION (provided by applicant): One major challenge in biomedical research is to leverage advances in genome sequencing into lead therapeutic modalities to treat human disease. This precision medicine approach holds great promise to advance patient-specific therapeutics and to provide highly selective chemical probes of function to study disease biology. In this proposal, we describe an innovative precision therapeutic approach to custom synthesize highly selective and potent lead therapeutics in only disease-affected cells and tissues by using a disease-causing gene product as a catalyst. That is, the disease-affected cell serves as a reaction vessel and a disease-causing RNA as a catalyst to allow for the synthesis of its own treatment. This is in contrast to traditional precision medicine approaches in which both healthy and disease-affected cells are exposed to the therapeutic, potentially causing toxicity due to binding off-targets. Our technology will be applied to develop compounds to treat and study microsatellite disorders that affect millions of people worldwide and have no known cure. Microsatellite disorders are caused by expanded repeating sequences located in both coding and non-coding regions, with the RNA being a key pathogenic agent. We have previously shown that repeating transcripts are most effectively targeted with multivalent compounds. However, as the compounds increase in valency, their molecular weights increase and their drug- likeness decreases. We therefore recently developed an innovative strategy to synthesize multivalent compounds, from their monovalent components, in cellulo using a disease-affected cell as a reaction vessel and a toxic, disease-causing RNA as a catalyst. We will take these exciting results in new directions and apply them to other debilitating microsatellite disorders including Huntington's disease, various forms of muscular dystrophy, the genetic defect that causes fragile X syndrome (the only known single gene cause of auti