Diffuse Intrinsic Pontine Glioma Genomic Study

从NCI和外部机构治疗的患者中收集了18个石蜡包埋的尸检标本组织块。目前正在进行病理学确认和审查。将从组织块中采集肿瘤的代表性区域。DNA将按照标准程序从核心中提取。将处理肿瘤DNA以进行高分辨率比较基因组杂交。 将使用阵列数据的循环二进制分割来识别拷贝数变化的区域。将识别标本中的共有区域，并询问与肿瘤进展相关的基因和途径。这项研究的结果在2009年的神经肿瘤学会会议上发表。这项研究将扩大到包括所有级别的神经胶质瘤。

Eighteen paraffin-embedded tissue blocks obtained from autopsy specimens have been collected from patients treated at NCI and outside institutions. These are currently undergoing pathology confirmation and review. A representative area of tumor will be collected from the block. DNA will be extracted from the core with standard procedures. Tumor DNA will be processed for high resolution comparative genomic hybridization. Regions of copy number change will be identified using circular binary segmentation of the array data. Consensus regions across specimens will be identified and interrogated for genes and pathways related to tumor progression. Results of this study were presented at the Society of Neuro-Oncology meeting 2009. The study will be expanded to include all grades of gliomas.