Collection and Taxonomy of Shallow Water Marine Organisms

承包商应独立地，而不是作为美国政府的代理，提供所有必要的服务、合格的人员、材料、设备和设施，而不是由政府另行提供，以收集足够数量的每年450-500个海洋大型生物，这些生物经过单独的提取设施处理后将产生10-20克的提取样品。从以前的研究来看，这将需要收集约1000克的冷冻湿重样品。收集的所有生物标本应提交至美国国家癌症研究所（NCI）的天然产物库（Frederick, Frederick， MD）存放。当收集者判断[I. 1]时，多达20%的生物可能以300至500克的数量提供。他们将提供足够的有机提取物或[II]。这种生物属于一个不寻常的分类群，数量并不多。必须采用无害生态的收集方法。收集后，尽快（通常在6个月内，但如果是“科学新手”，分类学家将尽可能迅速地进行物种鉴定）对收集到的每个样本进行物种鉴定。尽可能避免在整个合同期内从同一地点或附近地点（通常距离不到1公里）重复采集物种。藏品应包括尽可能广泛的门和纲的物种，不应集中于一个纲的太多物种而排除其他纲的物种。收集的主要是海洋无脊椎动物，大约10%的收集是海洋植物。脊椎动物被特别排除在收藏之外。由于先前的筛选表明它们的提取物要么完全无活性，要么剧毒，因此某些属和类应给予较低的优先级。天然产物库需要详细的收集文件。

Independently and not as an agent of the US Government, the contractor shall furnish all the necessary services, qualified personnel, material, equipment and facilities, not otherwise provided by the Government as needed to collect sufficient quantities of 450-500 marine macro-organisms per year which will yield 10-20 gram samples of extract after processing by a separate extraction facility. From previous studies, this would require the collection of ca 1000 grams, frozen wet weight of a given sample. Specimens of all organisms collected shall be submitted to the Natural Products Repository at the National Cancer Institute (NCI)-Frederick, Frederick, MD., for deposition. Up to 20% of the organisms may be provided in quantities from 300 to 500 grams when the collectors judge that [I.] they will provide adequate amounts of organic extract or [II.] the organism is from an unusual taxonomic group and is not abundant. Ecologically sound collection practices must be used. Undertake the complete identification to species level of every sample collected, as soon as possible (normally within 6 months but in the cases of "new to Science" then the taxonomist will proceed as expeditiously as is feasible to identify to species) after collection. Avoid, as far as is possible, duplicate collections of species over the full contract period from the same or close by sites [generally less than 1 kilometer away]. Collections shall include species from as wide a variety of phyla and classes as possible, and should not concentrate on too many species from one class to the exclusion of those from other classes. Collections shall be predominately marine invertebrates with up to approximately 10% of the collection being marine plants. Vertebrates are specifically excluded from the collection. Certain genera and classes shall be given low priority since prior screening has shown their extracts to be either totally inactive or highly toxic. Detailed documentation of the collection is required for the Natural Products Repository. In undertaking these collections, the National Cancer Institute (NCI) has committed itself to the conservation of biological diversity, as well as to policies of fair and equitable collaboration and compensation in interacting with the Source Countries participating in the collection programs. Agreements based on the NCI Letter of Collection have been signed with relevant government organizations in many of the source countries participating in the collection program. Each organism is extracted in the Natural Products Extraction Laboratory with a 1:1 mixture of dichloromethane and methanol, and then with water, and the extracts are stored at -200C in the Natural Products Repository. Both these facilities are operated by SAIC at the Frederick Cancer Research and Development Center under contract to the NCI. Both extracts are tested in the NCI cancer cell line screen in order to further research in cancer and cancer drug developmnet. The NCI considers the Natural Products Repository as a national resource, and extracts from the Repository are available either in vials or in 96 well plates for distribution to qualified organizations through the Open Repository Program and the Active Repository Program.