Analysis of complex biological systems by microarray based gene expression

通过基于基因表达的微阵列分析复杂的生物系统

• 批准号: 8149660
• 负责人: Kevin Becker
• 金额: $ 123.61万
• 依托单位: NATIONAL INSTITUTE ON AGING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8149660
• 关键词: Gene Expression; base; complex biological systems

The NIA microarray Core Facility: Molecular analysis of complex biological and disease processes has been transformed by the use of genome wide microarrays. The NIA microarray core facility provides gene expression services and analysis of gene expression data primarily to NIA intramural scientists and their collaborators. In addition, we collaborate directly with investigators in other NIH institutes as well as the extramural community. In fiscal year 2008 we completed 70 independent microarray experiments involving over 1,200 RNA samples. Our laboratory primarily uses Illumina bead chip microarrays for gene expression analysis and the hybridization and scanning equipment associated with the Illumina beadchip system. We also use Agilent, Exiqon, and other array formats as the experiment requires. We prepare RNA by a variety of methods and test RNA for quality and quantity using an Agilent 2100 Bioanalyzer. Bioinformatic analysis of microarray data is done with a variety of software programs and analytical approaches. These include BeadStudio, DIANE 6.0, GSEA, PAGE, Pathway Studio, Ingenuity Pathway Analysis, among others. Analysis is also supported with our analysis tools; PubMatrix, The Genetic Association database, and BBID. http://www.grc.nia.nih.gov/branches/rrb/dna/dna.htm, Disease/Phenotype webPAGE.

NIA微阵列核心设施： 全基因组微阵列的使用改变了复杂生物学和疾病过程的分子分析。NIA微阵列核心设施主要为NIA内部科学家及其合作者提供基因表达服务和基因表达数据分析。此外，我们直接与其他NIH研究所以及校外社区的研究人员合作。在2008财政年度，我们完成了70个独立的微阵列实验，涉及1,200多个RNA样本。 我们的实验室主要使用Illumina微珠芯片微阵列进行基因表达分析以及与Illumina微珠芯片系统相关的杂交和扫描设备。我们还根据实验需要使用Agilent，EQUIPQON和其他阵列格式。我们通过多种方法制备RNA，并使用Agilent 2100生物分析仪测试RNA的质量和数量。 微阵列数据的生物信息学分析是用各种软件程序和分析方法完成的。其中包括BeadStudio，DIANE 6.0，GSEA，PAGE，Pathway Studio，Influence Pathway Analysis等。我们的分析工具也支持分析; PubMatrix，遗传关联数据库和BBID。http：//www.grc.nia.nih.gov/branches/rrb/dna/dna.htm，疾病/表型网页。