Analysis of complex biological systems by microarray based gene expression

通过基于基因表达的微阵列分析复杂的生物系统

• 批准号: 8931665
• 负责人: Kevin Becker
• 金额: $ 99.62万
• 依托单位: NATIONAL INSTITUTE ON AGING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8931665
• 关键词: Bioinformatics; Biological; ChIP-seq; Communities; Complex; Computer software; Core Facility; DNA Sequence; Data; Data Analyses; Databases; Disease; Equipment; Extramural Activities; Gene Expression; Gene Expression Microarray Analysis; Genomics; Institutes; Laboratories; Methods; Microarray Analysis; Molecular Analysis; Pathway Analysis; Pathway interactions; Process; RNA; Research Personnel; Sampling; Scanning; Scientist; Services; System; Testing; United States National Institutes of Health; base; complex biological systems; disease phenotype; genetic association; genome-wide; next generation sequencing; programs; research study; tool; transcriptome sequencing

The NIA Gene Expression and Genomics Unit Core Facility: Molecular analysis of complex biological and disease processes has been transformed by the use of genome wide microarrays and with Next Generation Sequencing. The NIA Gene Expression and Genomics Unit core facility provides gene expression and NGS sequencing services and analysis of genomic data primarily to NIA intramural scientists and their collaborators. In addition, we collaborate directly with investigators in other NIH institutes as well as the extramural community. In fiscal year 2012 we completed 51 independent microarray experiments involving over 1,300 RNA samples. Our laboratory primarily uses Illumina bead chip microarrays for gene expression analysis and the hybridization and scanning equipment associated with the Illumina beadchip system. We also use Agilent, Exiqon, and other array formats as the experiment requires. We prepare RNA by a variety of methods and test RNA for quality and quantity using an Agilent 2100 Bioanalyzer. We have two NGS DNA sequencing systems, the Illumina GAII and the ION TORRENT PROTON. Bioinformatic analysis of microarray and DNA sequencing data is done with a variety of software programs and analytical approaches. These include BeadStudio, DIANE 6.0, GSEA, PAGE, Pathway Studio, Ingenuity Pathway Analysis, among others. Analysis is also supported with our analysis tools; PubMatrix, The Genetic Association database, BBID and Disease/Phenotype webPAGE.

NIA基因表达和基因组学单位核心设施： 通过使用全基因组微阵列和下一代测序，复杂的生物和疾病过程的分子分析已经发生了变化。NIA基因表达和基因组学单位的核心设施主要为NIA内部科学家及其合作者提供基因表达和NGS测序服务以及基因组数据分析。此外，我们还与其他NIH研究所以及校外社区的研究人员直接合作。在2012财年，我们完成了51个独立的微阵列实验，涉及1300多个RNA样本。 我们的实验室主要使用Illumina珠片芯片微阵列进行基因表达分析，以及与Illumina珠片系统相关的杂交和扫描设备。我们还根据实验需要使用Agilent、Exiqon和其他数组格式。我们通过各种方法制备RNA，并使用安捷伦2100生物分析仪对RNA进行定性和定量测试。我们有两个NGS DNA测序系统，Illumina GAII和离子洪流质子。 微阵列和DNA测序数据的生物信息学分析是通过各种软件程序和分析方法进行的。这些软件包括BeadStudio、Diane 6.0、GSEA、PAGE、Path Studio、Invenity Path Analysis等。我们的分析工具也支持分析；PubMatrix、遗传协会数据库、BBID和疾病/表型网页。