Analysis of complex biological systems by microarray and sequence based gene expression

通过微阵列和基于序列的基因表达分析复杂的生物系统

• 批准号: 10005792
• 负责人: Kevin Becker
• 金额: $ 159.29万
• 依托单位: NATIONAL INSTITUTE ON AGING
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10005792
• 关键词: Base Sequence; Bioinformatics; Biological; ChIP-seq; Communities; Complex; Computer software; Core Facility; DNA sequencing; Data; Data Analyses; Databases; Disease; Equipment; Extramural Activities; Gene Expression; Gene Expression Microarray Analysis; Genomics; Institutes; Laboratories; Methods; Microarray Analysis; Molecular Analysis; Pathway Analysis; Pathway interactions; Process; RNA; Research Personnel; Running; Sampling; Scanning; Scientist; Services; System; Testing; United States National Institutes of Health; base; complex biological systems; disease phenotype; experimental study; genetic association; genome-wide; genomic data; next generation sequencing; programs; tool; transcriptome sequencing; web page

The NIA Gene Expression and Genomics Unit Core Facility: Molecular analysis of complex biological and disease processes has been transformed by the use of genome wide microarrays and with Next Generation Sequencing. The NIA Gene Expression and Genomics Unit core facility provides gene expression and NGS sequencing services and analysis of genomic data primarily to NIA intramural scientists and their collaborators. In addition, we collaborate directly with investigators in other NIH institutes as well as the extramural community. In fiscal year 2019 we completed 18 independent microarray experiments involving over 774 RNA samples. Our laboratory primarily uses Agilent microarrays for gene expression analysis and the hybridization and scanning equipment associated with the Agilent microarray system. We also use other array formats as the experiment requires. We prepare RNA by a variety of methods and test RNA for quality and quantity using an Agilent 2100 Bioanalyzer. We use the NGS DNA sequencing system, Illumina Hi seq 2500 and have performed over 77 sequencing runs on over 951 samples. Bioinformatic analysis of microarray and DNA sequencing data is done with a variety of software programs and analytical approaches. These include BeadStudio, GSEA, PAGE, Pathway Studio, Ingenuity Pathway Analysis, among others. Analysis is also supported with our analysis tools; PubMatrix, The Genetic Association database, and Disease/Phenotype webPAGE.

NIA基因表达和基因组学单元核心设施： 通过使用全基因组微阵列和下一代测序，复杂生物和疾病过程的分子分析已经发生了变化。NIA基因表达和基因组学单元核心设施主要为NIA内部科学家及其合作者提供基因表达和NGS测序服务以及基因组数据分析。此外，我们直接与其他NIH研究所以及校外社区的研究人员合作。在2019财年，我们完成了18项独立的微阵列实验，涉及超过774个RNA样本。 我们的实验室主要使用安捷伦微阵列进行基因表达分析和与安捷伦微阵列系统相关的杂交和扫描设备。我们还根据实验需要使用其他数组格式。我们通过多种方法制备RNA，并使用Agilent 2100生物分析仪测试RNA的质量和数量。 我们使用NGS DNA测序系统Illumina Hi seq 2500，并对超过951个样品进行了超过77次测序。 微阵列和DNA测序数据的生物信息学分析是用各种软件程序和分析方法完成的。其中包括BeadStudio，GSEA，PAGE，Pathway Studio，Incidence Pathway Analysis等。我们的分析工具也支持分析; PubMatrix，遗传关联数据库和疾病/表型webPAGE。