Compact, Integrated EPR Spectrometer for Dynamic Nuclear Polarization

描述（由申请人提供）：拟研究的重点是开发一种紧凑，集成的高场EPR光谱仪，用于dnp增强固态，魔角旋转（MAS）核磁共振光谱。该光谱仪将允许研究人员在进行DNP实验的相同条件下记录EPR光谱并获得DNP样品的有价值的松弛参数。近年来，DNP在世界各地的实验室核磁共振实验中被证明是一种增强信号强度的有效方法，并且在将DNP应用于固态核磁共振光谱方面取得了实质性进展。然而，为了理解和优化DNP过程，充分了解极化剂的EPR谱和弛豫参数至关重要。世界上只有少数几台自制的高场光谱仪可用。因此，研究人员经常不得不推测其DNP样品的EPR特征。我们建议开发一种具有成本效益的EPR光谱仪，可以很容易地适应覆盖目前在DNP光谱中使用的整个频率范围。该设计将是紧凑和交钥匙，因此该设备也可以由非专业人士使用。在该项目的第一阶段，我们将进行完整的机械和电气设计，并制造一台工作在263 GHz （400 MHz 1H）的EPR光谱仪原型。这项技术的成功开发将为研究人员提供目前无法获得的EPR特性，并将促进新的、优化的DNP-NMR极化剂的开发。这将是许多由美国国立卫生研究院资助的项目非常感兴趣的。

DESCRIPTION (provided by applicant): The proposed research focuses on the development of a compact, integrated high-field EPR spectrometer for DNP-enhanced solid-state, magic-angle-spinning (MAS) NMR spectroscopy. The spectrometer will allow researchers to record the EPR spectra and obtain valuable relaxation parameters of the DNP sample under the same conditions under which the DNP experiment is performed. In recent years, DNP has proven to be a robust method to increase signal intensities in NMR experiments in laboratories around the world and substantial progress has been made in adapting DNP for solid-state NMR spectroscopy. However, to understand and optimize the DNP process it is crucial to fully understand the EPR spectrum and relaxation parameters of the polarizing agent. Only a few home-built high-field spectrometers are available around the world. Therefore researchers often have to speculate about the EPR characteristics of their DNP sample. We propose to develop a cost-effective EPR spectrometer that can easily be adapted to cover the complete frequency range that is currently used in DNP spectroscopy. The design will be compact and turn-key so that the device can also be used by non experts. During Phase I of this project we will perform the complete mechanical and electrical design and fabricate a prototype EPR spectrometer operating at 263 GHz (400 MHz 1H). The successful development of this technology will provide researchers access to currently inaccessible EPR characteristics and will proliferate the development of new, optimized polarizing agents for DNP-NMR spectroscopy. This will be of large interest to many projects funded by the U.S. National Institutes of Health.