Animal MRI/Imaging Core

动物 MRI/成像核心

• 批准号: 8177752
• 负责人: Andrew Arai
• 金额: $ 19.04万
• 依托单位: NATIONAL HEART, LUNG, AND BLOOD INSTITUTE
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/8177752
• 关键词: Acute; Aerobic Exercise; Age; Agonist; Anatomy; Animals; Aorta; Area; Arteries; Atlases; Blood Circulation; Blood Vessels; Breeding; Caliber; Cardiac; Cardiovascular Diseases; Cardiovascular system; Cell Aging; Cells; Chronic; Cicatrix; Collaborations; Collagen; Complex; Data; Defect; Development; Dilated Cardiomyopathy; Dimensions; Disease; Dobutamine; Electrocardiogram; Embryo; Equipment; Experimental Designs; Eye; Family suidae; Fibrosis; First Pregnancy Trimester; Gadolinium; Generations; Head; Heart Arrest; Histology; Human; Hydronephrosis; Image; Kidney; Knockout Mice; Life; Link; Magnetic Resonance; Magnetic Resonance Imaging; Measurement; Methods; Microscopy; Mission; Mitochondria; Mitochondrial DNA; Mitochondrial Proteins; Modeling; Mus; Myocardial; Myocardial Infarction; N-Acetylgalactosaminyltransferases; NIH Mouse; National Heart, Lung, and Blood Institute; National Human Genome Research Institute; National Institute of Child Health and Human Development; National Institute of Diabetes and Digestive and Kidney Diseases; National Institute of Neurological Disorders and Stroke; Nephrogenic Diabetes Insipidus; Nervous System Physiology; Nitrites; Paper; Phenotype; Ploidies; Principal Investigator; Printing; Progeria; Prostaglandin E Receptor; Proteins; Protocols documentation; Publishing; Rattus; Reactive Oxygen Species; Research; Research Design; Resolution; Rodent; Scanning; Scientist; Screening procedure; Severities; Site; Skeletal Muscle; Stem cells; Stress; Stress Tests; Structure; TP53 gene; Techniques; Testing; Therapeutic Effect; Time; Tissue Sample; Tissues; Transforming Growth Factor beta; Transgenic Mice; United States National Institutes of Health; Urography; Vascular Diseases; Vimentin; Yang; base; cardiogenesis; cardiovascular imaging; cell age; cellular imaging; fluorescence imaging; heart circulation; human WFDC2 protein; improved; in vivo; interest; mouse model; mutant; neutralizing antibody; non-muscle myosin; overexpression; polypeptide; renal artery; research study; shear stress; small molecule; treatment effect

Currently active projects, by Principal Investigator: 1. Arai A., NHLBI. Cardiac fibrosis, acute and chronic MI in rats. Microimaging scar tissue. (fellow Sivendran R.). 2. Adelstein R., NHLBI. Ongoing cardiac functional screening in various nonmuscle myosin mutants for identification of cardiac phenotypes, influence of age on phenotype and guidance of breeding (fellows Ma, Jana). 3. Bodine D., NHGRI: Progeria study, Collaboration with NHLBI: Drs. Nabel E., Cannon R.; NIH: Collins F.: Progeria study #2. Double copy mutant progeria model pilot and treatment studies with aorta imaging, diameter measurements and cardiac function. Primary scientist, Erdos M. 4. Boehm M., NHLBI. MI studies, scar identification and function, effect of treatment with TGF beta neutralizing antibody (fellows Elagha A., Yang D.). In addition a functional study was done to make a comparison to echo performed by the Phenotyping Core to compare techniques in evaluating function with a focal wall defect. 5. Horowits R., NIAMS. (fellow Crawford G.). Transgenic mice (N-RAP) to characterize for cardiac phenotype. Histology is suggestive of RV abnormality. Finding increasing severity of cardiac functonal abnormality, both LV and RV, via MRI with age, copy number. 6. Rochman M., Bustin, NCI: collaboration with Dr. Adestein. NSBP1 protein KO determine if there is a cardiac phenotype with or without stress. 7. Leonard W., NHLBI. 3D MRI examination of mouse head to examine the eyes and neighboring structures in a knockout mouse model. 8. Lo C. (former NHLBI PI). Imaging fixed embryos at 14T. 9. Finkel T., NHLBI. Cardiac function in a mouse model for studies of stem cells, aging and cardiovascular disease. Projects completed in 2009 by Principal Investigator: 1. Hwang P., NHLBI: p53: see Park JY et al. Circ. Res. 2009 2. Neufeld E., NHLBI: Imaging pig renal artery collagen for comparison to microscopy showing collagen formation at the artery junction. Testing determined that the collagen at the site did not incorporate a small molecule Gd chelate preferentially compared to the remainder of the vessel. 3. Sack M., NHLBI, dobutamine stress testing on mice with modified levels or lacking mitochondrial proteins park2 and/or pink1. (fellow Stevens) 4. Segars J., NICHD, fellow Guo C. (collaboration with Robert Adelstein): dobutamine stress on KO vs WT. Group is performing additional histopath and ECG analyses prior to concluding this study. 5. Wess J., NIDDK (collaboration with Mark Knepper, NHLBI): Kidney urography of an inducible KO causing hydronephrosis and therapeutic effect of a EP4 PGE2 receptor agonist: see Li JH et al. J.Clin. Invest. 2009 In addition, there are active ACUC protocols that are not being used currently that are eligible to perform studies at any time during the life of the protocol. Papers published in 2009, 2010: 1. Schelbert EB, Hsu L-Y, Anderson SA, Mohanty BD, Karim SM, Kellman P, Aletras AH, and Arai AE(NHLBI). Late Gadolinium Enhancement Cardiac Magnetic Resonance Identifies Post Infarction Myocardial Fibrosis and the Border Zone at Near Cellular Level in ex vivo Rat Heart. Circulation Cardiovascular Imaging, in press Sept 2010. 2. Yamada S, Samtani RR, Lee ES, Lockett E, Uwabe C, Shiota K, Anderson SA, Lo CW (2010) Developmental atlas of the early first trimester human embryo. Dev Dyn 239:1585-95. 3. Lau JF, Anderson SA, Adler E, Frank JA. Imaging approaches for the study of cell-based cardiac therapies. Nat Rev Cardiol. 2010 7(2):97-105. Epub 2009 Dec 22. 4. Li JH, Chou C-L, Li B, Gavrilova O, Eisner C, Schnermann J, Anderson SA, Deng C, Knepper KA & Wess J. A Selective EP4 PGE2 Receptor agonist alleviates disease in a new mouse model of X-linked nephrogenic diabetes insipidus. J. Clin. Invest. 119(10) 3115-26 (2009) Sept 1. Epub ahead of print 5. Dezfulian C, Shiva S, Alekseyenko A, Pendyal A, Beiser DG, Munasinghe JP, Anderson SA, Chesley CF, Vanden Hoek TL, Gladwin MT. Nitrite Therapy After Cardiac Arrest Reduces Reactive Oxygen Species Generation, Improves Cardiac and Neurological Function, and Enhances Survival via Reversible Inhibition of Mitochondrial Complex I. Circulation. 120(10) 897-905 (2009) Aug 24. Epub ahead of print 6. Park JY, Wang PY, Matsumoto T, Sung HJ, Ma W, Choi JW, Anderson SA, Leary SC, Balaban RS, Kang JG, Hwang PM p53 Improves Aerobic Exercise Capacity and Augments Skeletal Muscle Mitochondrial DNA Content. Circ Res. 105(7) 705-12 (2009) Aug 20. Epub ahead of print 7. Dhanantwari P, Lee E, Krishnan A, Samtani R, Yamada S, Anderson S, Lockett E, Donofrio M, Shiota K, Leatherbury L, Lo CW. Human cardiac development in the first trimester: a high-resolution magnetic resonance imaging and episcopic fluorescence image capture atlas.Circulation. 2009;120(4):343-51. Papers in submission or review, 2010: 1. Normal L Heart Development In Mice Is Dependent On Expression Of UDP-GalNAc:Polypeptide -N-Acetylgalactosaminyltransferase 1 Yu Guan (NIDDK), Stasia A. Anderson (NHLBI), Matthew F. Starost (DVR, NIH), Daryl J. Despres (NINDS) Timothy A. Fritz (NIDDK, FDA) and Lawrence A. Tabak (NIDDK). 2. Shear stress-induced vimentin deregulation and vasculopathy in a mouse model of progeria. Minjung Song,1 Hong San,2 Stasia A. Anderson1, Richard O. Cannon, III,1 Donald Orlic1 (NHLBI) Circulation Res. 3. Cardiac-Specific NRAP Overexpression Causes Dilated Cardiomyopathy in Mice Shajia Lu (NIAMS), Garland L. Crawford (NIAMS), Justin Dore (NIAMS), Stasia A. Anderson (NHLBI), Daryl DesPres (NINDS)and Robert Horowits (NIAMS).

首席研究员目前正在进行的项目： 1. Arai A.，NHLBI。大鼠心脏纤维化、急性和慢性心肌梗死。疤痕组织显微成像。 （Sivendran R. 研究员）。 2. Adelstein R.，NHLBI。正在对各种非肌肉肌球蛋白突变体进行心脏功能筛查，以鉴定心脏表型、年龄对表型的影响以及指导育种（Ma、Jana 研究员）。 3. Bodine D.，NHGRI：早衰症研究，与 NHLBI 合作：Drs.纳贝尔·E.，坎农·R.； NIH：Collins F.：早衰症研究#2。双拷贝突变型早衰症模型试点和治疗研究，包括主动脉成像、直径测量和心脏功能。首席科学家，Erdos M. 4. Boehm M.，NHLBI。 MI 研究、疤痕识别和功能、TGF β 中和抗体治疗的效果（研究员 Elagha A.、Yang D.）。此外，还进行了一项功能研究，以与表型核心执行的回波进行比较，以比较评估功能与局灶性壁缺陷的技术。 5.Horowits R.，NIAMS。 （克劳福德 G. 研究员）。用于表征心脏表型的转基因小鼠 (N-RAP)。组织学提示 RV 异常。通过 MRI 检查年龄、拷贝数，发现左心室和右心室心脏功能异常的严重程度不断增加。 6. Rochman M.，Bustin，NCI：与 Adestein 博士合作。 NSBP1 蛋白 KO 确定是否存在有应激或无应激的心脏表型。 7. 伦纳德·W.，NHLBI。对小鼠头部进行 3D MRI 检查，以检查基因敲除小鼠模型中的眼睛和邻近结构。 8. Lo C.（前 NHLBI PI）。 在 14T 下对固定胚胎进行成像。 9.芬克尔·T.，NHLBI。用于研究干细胞、衰老和心血管疾病的小鼠模型的心脏功能。 2009年首席研究员完成的项目： 1. Hwang P.，NHLBI：第 53 页：参见 Park JY 等人。循环。资源。 2009年 2. Neufeld E.，NHLBI：对猪肾动脉胶原进行成像，与显示动脉交界处胶原形成的显微​​镜进行比较。测试表明，与血管的其余部分相比，该部位的胶原蛋白没有优先结合小分子 Gd 螯合物。 3. Sack M.，NHLBI，对水平改变或缺乏线粒体蛋白park2和/或pink1的小鼠进行多巴酚丁胺应激测试。 （史蒂文斯同学） 4. Segars J.、NICHD、Guo C. 研究员（与 Robert Adelstein 合作）：KO 与 WT 上的多巴酚丁胺应激。在结束这项研究之前，小组正在进行额外的组织病理学和心电图分析。 5. Wess J.，NIDDK（与 Mark Knepper，NHLBI 合作）：引起肾积水的诱导型 KO 的肾尿路造影以及 EP4 PGE2 受体激动剂的治疗效果：参见 Li JH 等人。 J.克林。投资。 2009年 此外，还有一些目前尚未使用的有效 ACUC 方案，可以在方案有效期内的任何时间进行研究。 2009年、2010年发表论文： 1. Schelbert EB、Hsu L-Y、Anderson SA、Mohanty BD、Karim SM、Kellman P、Aletras AH 和 Arai AE（NHLBI）。晚期钆增强心脏磁共振可识别离体大鼠心脏的梗死后心肌纤维化和近细胞水平的边界区。 循环心血管成像，2010 年 9 月出版。 2. Yamada S, Samtani RR, Lee ES, Lockett E, Uwabe C, Shiota K, Anderson SA, Lo CW (2010) 早期妊娠人类胚胎发育图谱。开发动力 239：1585-95。 3.刘JF，安德森SA，阿德勒E，弗兰克JA。用于研究基于细胞的心脏疗法的成像方法。纳特·Rev Cardiol。 2010年7(2):97-105。电子版 2009 年 12 月 22 日。 4. Li JH、Chou C-L、Li B、Gavrilova O、Eisner C、Schnermann J、Anderson SA、Deng C、Knepper KA 和 Wess J。选择性 EP4 PGE2 受体激动剂可减轻新的 X 连锁肾性尿崩症小鼠模型的疾病。 J.克林。投资。 119(10) 3115-26 (2009) 9 月 1 日。印刷前的电子版 5. Dezfulian C、Shiva S、Alekseyenko A、Pendyal A、Beiser DG、Munasinghe JP、Anderson SA、Chesley CF、Vanden Hoek TL、Gladwin MT。心脏骤停后的亚硝酸盐治疗可减少活性氧的产生，改善心脏和神经功能，并通过可逆抑制线粒体复合物 I 循环来提高生存率。 120(10) 897-905 (2009) 8 月 24 日。印刷前的电子版 6. Park JY, Wang PY, Matsumoto T, Sung HJ, Ma W, Choi JW, Anderson SA, Leary SC, Balaban RS, Kang JG, Hwang PM p53 提高有氧运动能力并增强骨骼肌线粒体 DNA 含量。环路研究中心。 105(7) 705-12 (2009) Aug 20. 印刷前的电子版 7. Dhanantwari P、Lee E、Krishnan A、Samtani R、Yamada S、Anderson S、Lockett E、Donofrio M、Shiota K、Leatherbury L、Lo CW。人类心脏在妊娠早期的发育：高分辨率磁共振成像和落射荧光图像捕获图集。循环。 2009；120(4)：343-51。 2010年提交或审阅的论文： 1. 小鼠正常 L 心脏发育依赖于 UDP-GalNAc:多肽 α-N-乙酰半乳糖胺基转移酶 1 的表达 Yuguan (NIDDK)、Stasia A. Anderson (NHLBI)、Matthew F. Starost (DVR, NIH)、Daryl J. Despres (NINDS) Timothy A. Fritz (NIDDK, FDA) 和 Lawrence A. Tabak (NIDDK)。 2.早衰小鼠模型中剪切应力诱导的波形蛋白失调和血管病变。 Minjung Song,1 Hong San,2 Stasia A. Anderson1, Richard O. Cannon, III,1 Donald Orlic1 (NHLBI) 循环研究。 3. 心脏特异性 NRAP 过度表达导致小鼠扩张型心肌病 Shajia Lu (NIAMS)、Garland L. Crawford (NIAMS)、Justin Dore (NIAMS)、Stasia A. Anderson (NHLBI)、Daryl DesPres (NINDS) 和 Robert Horowits (NIAMS)。