PS-OC BIORESOURCE CORE FACILITY (PBCF)

PS-OC 生物资源核心设施 (PBCF)

• 批准号: 8174539
• 金额: $ 55万
• 依托单位国家: 美国
• 财政年份: 2010
• 资助国家: 美国
• 起止时间: 2010-09-24 至 2011-09-23
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8174539
• 关键词: Benchmarking; Biological; Breast Cancer Cell; Cancer cell line; Cancerous; Cell Culture Techniques; Cell Line; Cells; Communication; Core Facility; DNA; Data Set; Deposition; Ensure; Goals; Human Cell Line; Laboratories; Language; Malignant Neoplasms; Metric; Mission; Molecular; Non-Malignant; Participant; Pilot Projects; Proteins; Protocols documentation; Publications; Reagent; Research Personnel; Resources; Specimen; Testing; Time; Tissues; Work; biobank; cancer type; cost; follow-up; meetings; member; oncology; physical science; programs; reagent standard; research study; technology/technique

Each Physical Science ¿ Oncology Center (PS-OC) has unique capabilities in terms of its physical science approach to studying cancer. In order to develop a common language among the Centers, a pilot project was proposed to utilize common cell lines and standard operating protocols so that physical science metrics could be easily standardized across the PS-OC Network. To test the feasibility of providing an initial common benchmark, the pilot cell line project was initiated in November 2009. Participating laboratories within the PS-OC Network carried out and shared the results of a set of pilot experiments utilizing two (2) human breast cancer cell lines to facilitate inter-PS-OC communications and accelerate the understanding of PS-OC specific technologies and techniques. The cell lines were authenticated and distributed along with necessary cell culture reagents from a single laboratory to ensure that all participants were starting with the same biospecimens. Furthermore, a standard operating protocol was developed. Results of the cell line project were presented at the PS-OC First Annual Network Investigators¿ Meeting in April 2010 and also at a follow-up meeting in June 2010. A Network publication is anticipated as a result of this pilot project by 2011. From the PS-OC pilot cell line project, it has become apparent that utilization of cell lines and tissues with common reagents and standard operating protocols is critical for cross comparison of data sets. Being that the PS-OC Network investigators study at least eleven (11) different types of cancer, there is need for the establishment of a PS-OC Bioresource Core Facility (PBCF). The PBCF shall be a centralized biodistributor and biorepository that serves to provide PS-OC Network investigators with common stocks of authenticated cell lines and primary cells (non-malignant and cancerous), cell culture reagents and related standard operating protocols upon request. The PBCF shall also have the capability to prepare and distribute extracts of RNA, DNA or protein from human cell lines, primary cells and tissues. Moreover, any modified cell lines could be deposited by PS-OC Network investigators to the PBCF for authentication and distribution to collaborators within the PS-OC Network. The requirement outlined in this statement of work is a new requirement for the NCI OPSO, and it shall support both the mission of the PS-OC program and OPSO through providing a centralized resource of biological specimens for program investigators. The PBCF shall ultimately function to increase both the time and cost efficiency of the transfer of biological specimens to PS-OC Network investigators.

每个物理科学肿瘤中心（PS-OC）在其物理科学方法研究癌症方面都具有独特的能力。为了在各中心之间发展共同语言，提出了一个试点项目，利用共同的细胞系和标准操作方案，以便在整个PS-OC网络中轻松实现物理科学指标的标准化。为了测试提供初始共同基准的可行性，2009年11月启动了试验细胞系项目。PS-OC网络内的参与实验室利用两（2）种人类乳腺癌细胞系开展了一系列试点实验，并分享了实验结果，以促进PS-OC之间的交流，并加速对PS-OC特定技术和工艺的理解。对细胞系进行了认证，并与来自单个实验室的必要细胞培养试剂一起沿着分发，以确保所有参与者均从相同的生物标本开始。此外，还制定了标准操作规程。该细胞系项目的结果在2010年4月的PS-OC第一届年度网络调查员会议以及2010年6月的后续会议上进行了展示。预计到2011年，这一试点项目将产生一份网络出版物。 从PS-OC中试细胞系项目来看，很明显，使用通用试剂和标准操作方案的细胞系和组织对于数据集的交叉比较至关重要。由于PS-OC网络研究人员研究至少11种不同类型的癌症，因此需要建立PS-OC生物资源核心设施（PBCF）。PBCF应是一个集中的生物分销商和生物储存库，用于根据要求向PS-OC网络调查人员提供经认证的细胞系和原代细胞（非恶性和癌性）、细胞培养试剂和相关标准操作规程的普通库存。PBCF还应具有从人类细胞系、原代细胞和组织中制备和分配RNA、DNA或蛋白质提取物的能力。此外，任何经修饰的细胞系都可以由PS-OC网络研究者存放到PBCF，用于认证和分发给PS-OC网络内的合作者。 本工作说明书中概述的要求是对NCI OPSO的新要求，应通过为项目研究者提供集中的生物标本资源，支持PS-OC项目和OPSO的使命。PBCF的最终功能是提高向PS-OC网络调查人员转移生物标本的时间和成本效率。