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Engineering a Muscle Mimetic Biomaterial

工程模拟肌肉生物材料

基本信息

批准号：
8626694
负责人：
Jeffrey Wolchok
金额：
$ 43.72万
依托单位：
UNIVERSITY OF ARKANSAS AT FAYETTEVILLE
依托单位国家：
美国
项目类别：
财政年份：
2014
资助国家：
美国
起止时间：
2014-07-01 至 2018-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8626694
关键词：
Age Architecture Atrophic Attention Biocompatible Materials Biological Assay Blood Vessels Bone Marrow Cell Line Cells Collagen Collecting Cell Collection Connective Tissue Cues Defect Development Elasticity Elastin Engineering Extracellular Matrix Failure Fibroblasts Gender Glycosaminoglycans Growth Growth Factor Healed Healthcare Hydroxyproline Image Analysis Implant In Vitro Investments Left Life Measures Mechanics Methods Mimetic Muscles Modeling Muscle Muscle Cells Muscle Fibers Muscular Atrophy Natural regeneration Operative Surgical Procedures Performance Population Porosity Procedures Process Property Rattus Research Research Proposals Risk Factors Rotator Cuff Rupture Sampling Shapes Shoulder Skeletal Muscle Solvents Source Structure Techniques Tendon structure Testing Tissues United States Water design effective therapy engineering design fastins healing human tissue improved in vivo innovation interest mimetics muscle engineering muscle regeneration novel public health relevance regenerative repaired restoration scaffold skeletal sugar tibialis anterior muscle

项目摘要

Each year approximately 400,000 rotator cuff surgeries are performed in the United States. While operative techniques have improved, the re-rupture rate following rotator cuff repair remains high (30-35%). A recognized risk factor for tendon re-rupture is rotator cuff muscle degenerative atrophy. We suggest that a readily available "off the shelf" biomaterial that provides the appropriate regenerative cues by mimicking the properties of native skeletal muscle extracellular matrix could be used to regenerate damaged muscle tissue. To create a tissue mimetic material we have developed an innovative approach to capture the ECM secreted by populations of cells during growth in-vitro. This approach allows us to engineer materials that, like native tissues, are comprised entirely of cell derived constituents. The hypothesis of this research proposal is that we can use this ECM collection approach to build an engineered muscle mimetic (eMM) scaffold capable of regenerating damaged skeletal muscle. The following specific aims describe a development process in which the design of eMM scaffolds will be guided by the properties of native muscle tissue. Namely, skeletal muscle samples will be prepared and thoroughly characterized to serve as a design template (Aim 1). We will utilize our ECM collection approach to capture and organize the ECM secreted by muscle cells into an eMM scaffold with muscle mimetic composition and structure (Aim 2). We will test the regenerative performance of ECM scaffolds using a muscle defect model (Aim 3).

在美国，每年约有40万例肩袖手术。像手术尽管技术有所改进，但肩袖修复后的再破裂率仍然很高（30 - 35%）。一公认的肌腱再断裂的危险因素是肩袖肌肉退行性萎缩。我们建议，容易获得的"现成的"生物材料，其通过模仿天然骨骼肌细胞外基质的性质可用于再生受损的肌肉组织。为了创造一种组织模拟材料，我们开发了一种创新的方法来捕获分泌的ECM。在体外生长过程中的细胞群体。这种方法使我们能够设计材料，就像天然的一样，组织完全由细胞衍生成分组成。这项研究的假设是，我们可以使用这种ECM收集方法来构建工程肌肉模拟物（eMM）支架，再生受损的骨骼肌以下具体目标描述了一个发展进程， eMM支架的设计将由天然肌肉组织的性质指导。即骨骼肌将制备样品并对其进行彻底表征，作为设计模板（目标1）。我们将利用我们的ECM收集方法将肌细胞分泌的ECM捕获并组织成eMM支架具有肌肉模拟物的组成和结构（目的2）。我们将测试ECM的再生性能使用肌肉缺损模型的支架（目的3）。

项目成果

期刊论文数量（11）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Recovery from volumetric muscle loss injury: A comparison between young and aged rats.

DOI：
10.1016/j.exger.2016.07.008
发表时间：
2016-10
期刊：
EXPERIMENTAL GERONTOLOGY
影响因子：
3.9
作者：
Kim, John T.;Kasukonis, Benjamin M.;Brown, Lemuel A.;Washington, Tyrone A.;Wolchok, Jeffrey C.
通讯作者：
Wolchok, Jeffrey C.

The effect of autologous repair and voluntary wheel running on force recovery in a rat model of volumetric muscle loss.

DOI：
10.1113/ep089207
发表时间：
2021-04
期刊：
Experimental physiology
影响因子：
2.7
作者：
Washington TA;Perry RA Jr;Kim JT;Haynie WS;Greene NP;Wolchok JC
通讯作者：
Wolchok JC

Graft alignment impacts the regenerative response of skeletal muscle after volumetric muscle loss in a rat model.

DOI：
10.1016/j.actbio.2020.01.024
发表时间：
2020-03-15
期刊：
Acta biomaterialia
影响因子：
9.7
作者：
Kim J;Kasukonis B;Roberts K;Dunlap G;Brown L;Washington T;Wolchok J
通讯作者：
Wolchok J

Development of a biological scaffold engineered using the extracellular matrix secreted by skeletal muscle cells.

DOI：
10.1016/j.biomaterials.2015.01.027
发表时间：
2015-05
期刊：
BIOMATERIALS
影响因子：
14
作者：
Hurd, Shiloh A.;Bhatti, Nadia M.;Walker, Addison M.;Kasukonis, Ben M.;Wolchok, Jeffrey C.
通讯作者：
Wolchok, Jeffrey C.

In vivo testing of an injectable matrix gel for the treatment of shoulder cuff muscle fatty degeneration.