Single Cell RNA Sequencing (scRNA-seq) Instrumentation

项目摘要 我们建议购买一台台式细胞分选机，它将与细胞分离器配对，以建立一个 单细胞RNA测序（scRNA-seq）样品制备仪器 为调查人员提供最大的灵活性，并将成为阿肯色州大学（美国）唯一的同类仪器 校园和西北阿肯色州地区。该仪器将存放在工程研究中心内。 中心，一个具有支持其运作所需的基础设施和人员的设施。为支持这一 经过努力，确定了6个主要用户和12个次要用户，代表了最大的个人需求 单细胞分析能力的项目这些用户代表了成熟和早期职业生涯的混合 研究人员和反映单细胞分析的动态和不断增长的需求。在一起，主要和次要 用户拥有NIH（10）、NSF（4）、USDA（2）和州资源（2）的赠款，预计将包括 在第一年的运行中，约占仪器可持续用户时间（AUT）的85%。额外 预计其他试点项目用户的使用量较少（<1% AUT），但仍然有意义， scRNA-seq要求。拟议中的工具将成为美国充满活力的资源生态系统的一部分。 A.这些资源将共同确保scRNA-seq仪器的成功部署， 最大限度地利用它的研究社区的U。这一共享资源将获得巨大的财政和 行政支持，并接受咨询委员会的战略指导。该仪器将用于 推进与癌症，伤口愈合，衰老，糖尿病，炎症，神经科学， 和食品安全。这里提出的scRNA-seq仪器将对人类产生直接和持久的影响。 亚利桑那大学的研究生产力，允许用户设计更稳健的实验，更有效地利用 拨款美元，并进行最好的科学可能，与大学的利益是一致的 致力于发展其生物医学研究奋进。

PROJECT SUMMARY We propose to acquire a benchtop cell sorter which will be paired with a cell partitioner in order to establish a single cell RNA sequencing (scRNA-seq) sample preparation instrument This shared instrumentation provides maximum flexibility for investigators and will be the only instrument of its kind on the University of Arkansas (U of A) campus and Northwest Arkansas area. The instrument will be housed within the Engineering Research Center, a facility with the infrastructure and personnel necessary to support its operation. In support of this effort, a cohort of 6 Major and 12 Minor Users have been identified that represent the largest individual needs for single cell analysis capabilities at the U of A. These users represent a mix of established and early-career investigators and reflect the dynamic and growing need for single cell analysis. Together, the Major and Minor users hold grants from the NIH (10), NSF (4), USDA (2) and State sources (2) and are projected to comprise approximately 85% of the instrument’s Accessible User Time (AUT) in the first year of operation. Additional usage is anticipated from additional pilot project users with less substantial (<1% AUT), but still meaningful, scRNA-seq requirements. The proposed instrument will be part of a vibrant ecosystem of resources at the U of A. Together, these resources will ensure the successful deployment of the scRNA-seq instrument and maximize its usage by the U of A research community. This shared resource will enjoy significant financial and administrative support and receive strategic guidance from the Advisory Board. The instrument will be used to advance numerous projects related to cancer, wound healing, aging, diabetes, inflammation, neuroscience, and food safety. The scRNA-seq instrument proposed here will have an immediate and lasting impact on research productivity at the U of A, allowing users to design more robust experiments, make more efficient use of grant dollars, and conduct the best science possible, benefits that are consistent with the University’s commitment to growing its biomedical research endeavor.