Daily life and proinflammatory gene expression

描述(由申请人提供)：家庭的情感和社会生活影响儿童的健康，对成人的发病率和死亡率有明显的持久影响。特别是，家庭冲突和愤怒，缺乏养育，以及冷淡和不支持的家庭关系--所谓的“危险”特征--与健康状况不佳有关。研究文献中仍然缺少对危险家庭所通过的特定生物学途径的测试 影响健康，特别是在细胞水平上，尽管可能的候选者是炎症生物学。这项拟议的研究将利用作为我们加州大学洛杉矶分校家庭和健康研究的一部分收集的数据来测试危险的家庭环境和对感染的增强先天免疫反应之间的联系，这种先天免疫反应被称为促炎表型。我们将在细胞水平上检测致炎表型，重点研究炎症相关基因在白细胞中的表达。血液样本来自47个家庭的父母和9-13岁的目标儿童。在每年的感冒/流感季节，父母和目标儿童完成应激生活事件访谈，并参与为期8周的每日日记研究，评估家庭互动、儿童生活中的日常事件，以及儿童的情绪、行为、URI症状和健康行为。在为期8周的日记结束后，又对URI症状进行了8周的监测。在第3周和第6周的4天内每天采集唾液4次，检测唾液皮质醇。在家访期间，训练有素的人员对疑似URI进行了临床核实。在日记阶段之后，我们从父母和目标儿童那里获取了血液样本。这个项目将使用尖端的社会基因组学方法来检测白细胞中的基因表达。在这项研究中，每日日记方法的使用解决了文献中的一个重要空白，允许检查家庭环境的每日变化及其对炎症基因表达的影响。

DESCRIPTION (provided by applicant): The emotional and social lives of families influence children's health with lasting effects evident in adult morbidity and mortality. In particular, ovet family conflict and anger, deficient nurturing, and family relationships that are cold and unsupportive - so-called "risky" characteristics - are associated with poor health. Still missing from the research literature are tests of specific biological pathways through which risky families impact health, particularly at the level of the cell, although a likely candidate is inflammatory biology. The proposed study will utilize data collected as part of our UCLA Families and Health Study to test links between risky family environments and enhanced innate immune responses to infection, termed a proinflammatory phenotype. We will examine the proinflammatory phenotype at the cellular level, focusing on expression of inflammation-related genes in leukocytes. Blood samples were collected from parents and a target child between 9 - 13 years of age in 47 families. During the annual cold/flu season, parents and the target child completed stressful life event interviews and participated in an 8-week daily diary study assessing family interactions, daily events in the child's life, and child mood, behavior, URI symptoms, and health behaviors. URI symptoms were monitored for an additional 8 weeks after the 8-week diary. Saliva was sampled 4 times/day during 4 days in week 3 and 6 for salivary cortisol. Suspected URIs were clinically verified by trained personnel during home visits. Following the diary phase we obtained blood samples from parents and the target child. This project will examine gene expression in leukocytes using cutting-edge social genomics approaches. The use of daily diary methodology in this study addresses an important gap in the literature by permitting the examination of daily variations in family environments and their effects on inflammatory gene expression.