Daily life and proinflammatory gene expression

描述（由申请人提供）：家庭的情感和社交生活影响儿童的健康，对成人发病率和死亡率产生明显的持久影响。特别是，过度的家庭冲突和愤怒、缺乏养育以及冷漠和不支持的家庭关系——所谓的“危险”特征——与健康状况不佳有关。研究文献中仍然缺少对高风险家庭所通过的特定生物学途径的测试。 影响健康，特别是在细胞水平上，尽管可能的候选者是炎症生物学。拟议的研究将利用加州大学洛杉矶分校家庭与健康研究收集的数据来测试危险的家庭环境与增强的感染先天免疫反应（称为促炎表型）之间的联系。我们将在细胞水平上检查促炎表型，重点关注白细胞中炎症相关基因的表达。 血液样本采集自 47 个家庭的父母和 9 至 13 岁的目标儿童。在每年的感冒/流感季节，父母和目标孩子完成了压力生活事件访谈，并参加了为期 8 周的每日日记研究，评估家庭互动、孩子生活中的日常事件以及孩子的情绪、行为、URI 症状和健康行为。在 8 周日记之后，再监测 URI 症状 8 周。在第3周和第6周的4天内，每天对唾液进行4次采样，以检测唾液皮质醇。可疑的 URI 由训练有素的人员在家访期间进行临床验证。在日记阶段之后，我们从父母和目标孩子那里获取了血液样本。该项目将使用尖端的社会基因组学方法检查白细胞中的基因表达。本研究中使用每日日记方法可以检查家庭环境的日常变化及其对炎症基因表达的影响，从而弥补了文献中的一个重要空白。

DESCRIPTION (provided by applicant): The emotional and social lives of families influence children's health with lasting effects evident in adult morbidity and mortality. In particular, ovet family conflict and anger, deficient nurturing, and family relationships that are cold and unsupportive - so-called "risky" characteristics - are associated with poor health. Still missing from the research literature are tests of specific biological pathways through which risky families impact health, particularly at the level of the cell, although a likely candidate is inflammatory biology. The proposed study will utilize data collected as part of our UCLA Families and Health Study to test links between risky family environments and enhanced innate immune responses to infection, termed a proinflammatory phenotype. We will examine the proinflammatory phenotype at the cellular level, focusing on expression of inflammation-related genes in leukocytes. Blood samples were collected from parents and a target child between 9 - 13 years of age in 47 families. During the annual cold/flu season, parents and the target child completed stressful life event interviews and participated in an 8-week daily diary study assessing family interactions, daily events in the child's life, and child mood, behavior, URI symptoms, and health behaviors. URI symptoms were monitored for an additional 8 weeks after the 8-week diary. Saliva was sampled 4 times/day during 4 days in week 3 and 6 for salivary cortisol. Suspected URIs were clinically verified by trained personnel during home visits. Following the diary phase we obtained blood samples from parents and the target child. This project will examine gene expression in leukocytes using cutting-edge social genomics approaches. The use of daily diary methodology in this study addresses an important gap in the literature by permitting the examination of daily variations in family environments and their effects on inflammatory gene expression.