Daily life and proinflammatory gene expression

日常生活与促炎症基因表达

• 批准号: 8848095
• 负责人: Theodore Robles
• 金额: $ 7.51万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-05-12 至 2016-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8848095
• 关键词: 13 year old; Acute; Address; Adult; Aging; Anger; Applications Grants; Behavior; Biological; Biological Process; Biology; Blood; Blood specimen; Cells; Characteristics; Child; Child health care; Childhood; Chronic Disease; Clinical; Collection; Conflict (Psychology); Data; Data Analyses; Data Collection; Emotional; Environment; Event; Family; Family Relationship; Family health status; Foundations; Funding; Gene Expression; Genes; Genomic approach; Glucocorticoid Receptor; Glucocorticoids; Grant; Health; Health behavior; Home environment; Home visitation; House Call; Human Resources; Hydrocortisone; Immune; Immune response; Infection; Inflammation; Inflammatory; Interview; Laboratories; Leukocytes; Life; Link; Literature; Measures; Mediating; Methodology; Modeling; Monitor; Moods; Morbidity - disease rate; Outcome; Output; Parents; Pathway interactions; Phase; Phenotype; Process; Publishing; Questionnaires; RNA; Relative (related person); Reporting; Research; Resistance; Saliva; Salivary; Sampling; Seasons; Signal Transduction; Socioeconomic Status; Surveys; Symptoms; Testing; Time; Training; Upper Respiratory Infections; Variant; Viral; base; design; diaries; family influence; flu; gene repression; genome-wide; mortality; physical conditioning; receptor binding; response; social; social genomics; stressor; transcriptomics

DESCRIPTION (provided by applicant): The emotional and social lives of families influence children's health with lasting effects evident in adult morbidity and mortality. In particular, ovet family conflict and anger, deficient nurturing, and family relationships that are cold and unsupportive - so-called "risky" characteristics - are associated with poor health. Still missing from the research literature are tests of specific biological pathways through which risky families impact health, particularly at the level of the cell, although a likely candidate is inflammatory biology. The proposed study will utilize data collected as part of our UCLA Families and Health Study to test links between risky family environments and enhanced innate immune responses to infection, termed a proinflammatory phenotype. We will examine the proinflammatory phenotype at the cellular level, focusing on expression of inflammation-related genes in leukocytes. Blood samples were collected from parents and a target child between 9 - 13 years of age in 47 families. During the annual cold/flu season, parents and the target child completed stressful life event interviews and participated in an 8-week daily diary study assessing family interactions, daily events in the child's life, and child mood, behavior, URI symptoms, and health behaviors. URI symptoms were monitored for an additional 8 weeks after the 8-week diary. Saliva was sampled 4 times/day during 4 days in week 3 and 6 for salivary cortisol. Suspected URIs were clinically verified by trained personnel during home visits. Following the diary phase we obtained blood samples from parents and the target child. This project will examine gene expression in leukocytes using cutting-edge social genomics approaches. The use of daily diary methodology in this study addresses an important gap in the literature by permitting the examination of daily variations in family environments and their effects on inflammatory gene expression.

描述（由申请人提供）：家庭的情感和社会生活影响儿童的健康，对成人发病率和死亡率有明显的持久影响。特别是，过度的家庭冲突和愤怒，缺乏养育，以及冷漠和不支持的家庭关系-所谓的“危险”特征-与健康状况不佳有关。研究文献中仍然缺少对特定生物学途径的测试，通过这些途径， 影响健康，特别是在细胞水平上，尽管可能的候选者是炎症生物学。这项拟议的研究将利用作为我们加州大学洛杉矶分校家庭和健康研究的一部分收集的数据来测试危险的家庭环境和增强的先天免疫反应之间的联系，称为促炎表型。我们将在细胞水平上研究促炎表型，重点是白细胞中炎症相关基因的表达。 从47个家庭的父母和一名9 - 13岁的目标儿童那里收集了血液样本。在每年的感冒/流感季节，父母和目标儿童完成压力生活事件访谈，并参加了为期8周的每日日记研究，评估家庭互动，儿童生活中的日常事件，儿童情绪，行为，URI症状和健康行为。在8周日记后，再监测URI症状8周。在第3周和第6周的4天内，对唾液皮质醇进行4次/天采样。可疑URI由经过培训的人员在家访期间进行临床验证。在日记阶段之后，我们从父母和目标儿童那里获得了血液样本。该项目将使用尖端的社会基因组学方法研究白细胞中的基因表达。在这项研究中使用每日日记的方法，允许检查家庭环境中的日常变化及其对炎症基因表达的影响，从而解决了文献中的一个重要空白。

项目成果

Family environments and leukocyte transcriptome indicators of a proinflammatory phenotype in children and parents.

儿童和父母促炎表型的家庭环境和白细胞转录组指标。

• DOI: 10.1017/s0954579417000591
• 发表时间: 2018
• 期刊: Development and psychopathology
• 影响因子: 3.3
• 作者: Robles,TheodoreF;Repetti,RenaL;Reynolds,BridgetM;Chung,PaulJ;Arevalo,JesusaMG;Cole,StevenW
• 通讯作者: Cole,StevenW