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Natural Wolbachia infections for the control of Anopheles mosquitoes

用于控制按蚊的天然沃尔巴克氏体感染

基本信息

批准号：
8872456
负责人：
Flaminia Catteruccia
金额：
$ 24.23万
依托单位：
HARVARD SCHOOL OF PUBLIC HEALTH
依托单位国家：
美国
项目类别：
财政年份：
2015
资助国家：
美国
起止时间：
2015-01-15 至 2016-12-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/8872456
关键词：
Affect Africa Anopheles Genus Anopheles gambiae Area Arthropods Bacteria Bacterial Genome Bacterial Infections Bite Burkina Faso Cause of Death Cell Culture Techniques Cell Line Chemicals Child Competence Culicidae Data Detection Development Diagnostic Procedure Disease Embryo Female Fluorescent in Situ Hybridization Future Genes Genome Genomics Goals Individual Infection Infection Control Inherited Insecta Insecticide Resistance Insecticides Invaded Knowledge Laboratories Laboratory Infection Longevity Malaria Malaria Vaccines Methods Microinjections Molecular Molecular Genetics Mosquito Control Names Ovary Parasites Phenotype Phylogenetic Analysis Plasmodium Population Pregnant Women Prevalence Prevalence Study Research Residual state Resistance Rest Risk Sampling Shotguns Sterility System Techniques Testing Tissues Validation Wolbachia base co-infection endosymbiont feeding fighting fitness innovation killings malaria transmission male metagenomic sequencing neglect novel novel strategies pathogen public health relevance reproductive tool vector vector control vector mosquito weapons

项目摘要

DESCRIPTION (provided by applicant): Malaria is transmitted by the bite of the female Anopheles mosquitoes that are infected with Plasmodium parasites. This disease kills almost a million people each year, mostly pregnant women and young children in Africa. In 2012 there were 200 million people infected with malaria, and 3.3 billion people were at risk of infection. In the absence of an effective malaria vaccine, chemical control of the mosquito vector through the use of insecticides remains the best weapon to fight malaria transmission. Insecticide resistance in mosquitoes is however on the rise, and novel methods to control mosquito populations are urgently needed. As an alternative tool for malaria control, the use of Wolbachia endosymbionts has been widely proposed as these bacterial infections are able to spread rapidly through insect populations and render hosts resistant to co-infections with disease-causing pathogens, including Plasmodium. Previous efforts to find these bacteria in Anopheles species were unsuccessful, however we recently discovered a novel Wolbachia strain, which we called wAnga, in natural populations of several species of Anopheles mosquitoes in Burkina Faso, West Africa. In this project we will considerably expand our knowledge of this natural Wolbachia infection in Anopheles mosquitoes, and will generate a formidable toolkit for future studies aimed at assessing the use of these bacteria for malaria control. We will culture wAnga in cell lines and we will sequence the bacterial genome. We will generate stably infected mosquito lines and will test key reproductive phenotypes potentially induced by Wolbachia. Moreover, we will develop a rapid and specific method to facilitate detection of infected mosquitoes in field settings that will be instrumental to study the prevalence and distribution of infection in field mosquito populations. This highly innovative proposal will open up a new area of research and offers an exceptional opportunity to further endosymbiont-based vector control methods for Anopheles species.