Crosstalk between membrane traffic proteins and integrin activation

膜运输蛋白和整合素激活之间的串扰

• 批准号: 8837170
• 负责人: ZHENYU Li
• 金额: $ 37.5万
• 依托单位: UNIVERSITY OF KENTUCKY
• 依托单位国家: 美国
• 财政年份: 2014
• 资助国家: 美国
• 起止时间: 2014-09-25 至 2018-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8837170
• 关键词: Actins; Acute; Adhesions; Adhesives; Anabolism; Arthrogryposis; Atherosclerosis; Binding; Binding Proteins; Biochemical; Blood Platelets; Blood Vessels; Cause of Death; Cell Adhesion; Cell surface; Cells; Chinese Hamster; Chinese Hamster Ovary Cell; Cholestasis; Co-Immunoprecipitations; Coagulation Process; Complex; Confocal Microscopy; Cytomegalovirus; Cytoplasmic Granules; Cytoplasmic Organelle; Cytoplasmic Tail; Data; Defect; Development; Disease; Endocytosis; Epitopes; Event; Exocytosis; Extracellular Matrix; Fibrin; Fibrinogen; Functional disorder; Goals; Health; Hemostatic function; Host Defense; Human; Inflammation; Integrin Binding; Integrins; Kidney; Knockout Mice; Ligands; Link; MAP Kinase Gene; Malignant Neoplasms; Maps; Mediating; Megakaryocytes; Membrane Fusion; Membrane Protein Traffic; Membrane Proteins; Modeling; Molecular; Mouse Strains; Mus; Myocardial Infarction; Ovary; P-Selectin; Pathway interactions; Patients; Phosphorylation; Plasma; Platelet Activation; Play; Process; Protein Family; Proteins; Recombinants; Research Project Grants; Role; SRC gene; Signal Pathway; Signal Transduction; Stroke; Syndrome; Testing; Thrombosis; Time; United States; VWF gene; Work; Wound Healing; angiogenesis; antimicrobial; base; extracellular; in vivo; insight; member; mouse model; mutant; novel; overexpression; polymerization; prevent; promoter; receptor; receptor mediated endocytosis; recombinase; research study; rho GTP-Binding Proteins; stem; syntaxin binding protein 1

DESCRIPTION (provided by applicant): Crosstalk between membrane traffic proteins and integrin activation DESCRIPTION (provided by applicant): Platelets are central to hemostasis because they respond to vascular damage and secrete a variety of granule cargo molecules, which are critical to thrombosis and its sequellae. Platelet secretion is a multistep process involving centralization of cytoplasmic organelles that provides a contractile force, membrane fusion controlled by membrane traffic proteins, and release of granule contents. The platelet integrin, αIIbβ3 interacts with adhesive ligands such as fibrinogen and fibrin and mediates platelet adhesion and aggregation and thus plays a critical role in the development of thrombotic diseases such as heart attack and stroke. The overall goal of this proposal is to establish a link between the membrane traffic proteins and integrins that involve the two key events to platelet function, platelet cargo release and integrin activation. VPS33B is a member of the Sec1/Munc18 protein family that has multiple roles in exocytosis. VPS33B is defective in patients with arthrogryposis, renal dysfunction, cholestasis (ARC) syndrome. Platelets from these patients lack α granules. In an attempt to define the roles of α granules in platelet functio and thrombosis, we produced a mouse model of α granule deficiency, in which VPS33B was deleted only in megakaryocytes and platelets. In Specific Aim 1, we will determine the roles of VPS33B in αIIbβ3 dependent endocytosis and αIIbβ3 outside-in signaling. α granule contents vWF and fibrinogen are significantly reduced in the platelets lacking VPS33B. Surprisingly, VPS33B-/- platelets fail to retract a clot and are defective in spreading on fibrinogen. Using the CHO recombinant αIIbβ3activation model, we showed that overexpression of VPS33B markedly potentiates cell spreading on fibrinogen and actin polymerization. Thus, we hypothesize that in platelets, there exists a crosstalk between the membrane traffic proteins and integrin activation and that VPS33B is a key contributor to αIIbβ3 outside-in signaling. This hypothesis will be tested using the platelet- specific VPS33B conditional knockout mice and other VPS33B knockout mice such as whole-body knockout mice and a Chinese Hamster Ovary (CHO) integrin activation model. Our preliminary data demonstrated that VPS33B co-localizes with αIIbβ3 as detected by confocal microscopy and forms a complex with αIIbβ3 as detected by co-immunoprecipitation. In Specific Aim 2 we will use multiple approaches to characterize the binding determinants in VPS33B and theα cytoplasmic domains for each other. In Specific Aim 3 we will identify the molecular mechanisms of VPS33B-dependent αIIbβ3 outside-in signaling. Completion of these studies will not only identify a novel αIIbβ3 binding partner that plays an important role in αIIbβ3 outside-in signaling, but also for the first time establish a lin between membrane traffic proteins and integrin activation in platelets.

描述（由申请人提供）：膜运输蛋白和整合素活化之间的串扰描述（由申请人提供）：血小板是止血的核心，因为它们对血管损伤有反应并分泌各种颗粒货物分子，这对血栓形成及其后遗症至关重要。血小板分泌是一个多步骤的过程，涉及提供收缩力的细胞质细胞器的集中化、由膜运输蛋白控制的膜融合和颗粒内容物的释放。血小板整合素αIIbβ3与粘附配体如纤维蛋白原和纤维蛋白相互作用，介导血小板粘附和聚集，因此在血栓性疾病如心脏病发作和中风的发展中起关键作用。该提案的总体目标是建立膜运输蛋白和整合素之间的联系，其涉及血小板功能的两个关键事件，血小板货物释放和整合素活化。VPS 33 B是Sec 1/Munc 18蛋白家族的成员，在胞吐中具有多种作用。VPS 33 B在关节弯曲、肾功能不全、胆汁淤积（ARC）综合征患者中存在缺陷。这些患者的血小板缺乏α颗粒。为了明确α颗粒在血小板功能和血栓形成中的作用，我们建立了α颗粒缺陷小鼠模型，其中VPS 33 B仅在巨核细胞和血小板中缺失。在具体目标1中，我们将确定VPS 33 B在αIIbβ3依赖性内吞作用和αIIbβ3由外向内信号传导中的作用。VPS 33 B缺失的血小板α颗粒含量、vWF和纤维蛋白原均显著降低。令人惊讶的是，VPS 33 B-/-血小板不能收缩凝块，并且在纤维蛋白原上的铺展方面有缺陷。使用CHO重组αIIbβ 3激活模型，我们发现VPS 33 B的过表达显著增强了纤维蛋白原和肌动蛋白聚合上的细胞铺展。因此，我们假设在血小板中，在膜运输蛋白和整合素活化之间存在串扰，并且VPS 33 B是αIIbβ3由外向内信号传导的关键贡献者。将使用血小板特异性VPS 33 B条件性敲除小鼠和其他VPS 33 B敲除小鼠（如全身敲除小鼠和中国人卵巢（CHO）整联蛋白活化模型）来检验该假设。我们的初步数据表明，VPS 33 B与αIIbβ3共定位，如通过共聚焦显微镜检测到的，并与αIIbβ3形成复合物，如通过免疫共沉淀检测到的。在具体目标2中，我们将使用多种方法来表征VPS 33 B和α胞质结构域中的相互结合决定簇。在具体目标3中，我们将确定VPS 33 B依赖性αIIbβ3外向信号传导的分子机制。这些研究的完成不仅将鉴定在αIIb β3由外向内信号传导中起重要作用的新型αIIb β3结合伴侣，而且还将首次建立血小板膜运输蛋白与整合素活化之间的联系。