MRI-Guided Irreversible Electroporation Ablation for Liver Tumors

 描述(申请人提供)：不可逆电穿孔(IRE)是一种强大的新消融技术，它克服了热消融方法的许多主要限制。然而，为了准确、早期地预测治疗反应，后续成像方法必须允许在体内区分不可逆的电穿孔组织和治疗不充分的可逆电穿孔组织。我们最新的研究表明，定量MRI方法可以检测到电穿孔引起的肿瘤和正常组织细胞膜的变化。我们将开发一种强大的新的MRI引导的IRE方法，允许a)术前计划预测IRE消融量，b)术中监测组织反应，c)术后早期识别未充分治疗(可逆性电穿孔)的组织。我们的最终目标是实现靶向肿瘤的完全治疗，同时避免对邻近肝组织造成过度损害。拟议的临床前项目将在一个成熟的兔肝肿瘤模型中解决以下特定目标：特定目标1：开发程序前计划技术以更准确地预测IRE消融量。具体目标2：发展用于即时监测肿瘤组织反应的术中成像方法。具体目标3：开发手术后成像方法，用于早期识别治疗不充分、可逆性电穿孔的肿瘤组织。

 DESCRIPTION (provided by applicant): Irreversible electroporation (IRE) is a powerful new ablation technology that overcomes many of the primary limitations of thermal ablation approaches. However, for accurate, early prediction of treatment response, follow-up imaging methods must permit in vivo discrimination of irreversibly electroporated tissues from adjacent inadequately treated, reversibly electroporated tissues. Our most recent studies have demonstrated that quantitative MRI methods can detect electroporation-induced alterations to the cell membrane in both tumor and normal tissues. We will develop a powerful new MRI-guided IRE approach permitting a) pre- procedural planning to predict IRE ablation volumes, b) intra-procedural monitoring of tissue response and c) early post-procedural identification of inadequately treated (reversibly electroporated) tissues. Our ultimate goal is to achieve complete treatment of targeted tumors while avoiding excessive damage to adjacent liver tissues. The proposed pre-clinical project will address the following Specific Aims in a well-established rabbit liver tumor model: Specific Aim 1: To develop pre-procedural planning techniques to more accurately predict IRE ablation volumes. Specific Aim 2: To develop intra-procedural imaging methods for immediate functional monitoring of tumor tissue response. Specific Aim 3: To develop post-procedural imaging methods for early identification of inadequately treated, reversibly electroporated tumor tissues.