Analytical - Core B

抽象-核心B 这一核心侧重于基因、蛋白质、多肽和小药物的定性和定量分析。 使用常规生化方法的化合物与高灵敏质谱仪(MS)联用 和激光捕获显微解剖(LCM)进行样本采集。它将作为LCM的中心资源，并 中枢神经系统样品共价蛋白质修饰的QRT-PCR(UNTHSC)和MS分析 3个项目。岩心将从调查人员那里接收原始样本，这些样本通常以 来自特定大脑感兴趣区域的击打。LCM将用于从 大脑切片允许项目使用比打孔更具体的样本进行实验 样本。逆行追踪、细胞旁标记、免疫组织化学和报告基因的表达 分子将被用来识别感兴趣的神经元。

Abstract – Core B This core focuses on qualitative and quantitative analyses of genes, proteins, peptides and small drug compounds using conventional biochemical methods coupled with highly sensitive mass spectrometry (MS) and Laser Capture Microdissection (LCM) for sample collection. It will serve as a central resource for LCM and qRT-PCR (UNTHSC) and MS analysis of covalent protein modifications (UTHSCSA) of CNS samples from the 3 projects. The core will receive raw samples from investigators, most often these will be in the form of punches from specific brain regions of interest. LCM will be used to harvest defined populations of cells from brain sections allowing the projects to conduct experiments using samples that are more specific than punched samples. Retrograde track tracing, juxtacellular labeling, immunohistochemistry, and the expression of reporter molecules will be used to identify neurons of interest.