Analytical - Core B

分析 - 核心 B

• 批准号: 8935552
• 负责人: J Thomas Cunningham
• 金额: $ 31.7万
• 依托单位: UNIVERSITY OF NORTH TEXAS HLTH SCI CTR
• 依托单位国家: 美国
• 财政年份: 2008
• 资助国家: 美国
• 起止时间: 2008-09-05 至 2020-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8935552
• 关键词: Accounting; Anatomy; Antibodies; Area; Biochemical; Biological Assay; Brain region; Cardiovascular system; Chronic; Core Facility; Coupled; Data Analyses; Data Files; Databases; Drug Compounding; Equipment; Functional disorder; Gene Proteins; Genomics; Harvest; Health Sciences; Human Resources; Hypoxia; Immunohistochemistry; Instruction; Label; Mass Spectrum Analysis; Methods; Mission; Neurons; Peptides; Physiology; Population; Post-Translational Protein Processing; Preparation; Procedures; Process; Proteins; Proteomics; RNA; Reagent; Reporter; Research; Research Institute; Research Personnel; Resources; Sampling; Schedule; Secure; Site; Sleep Apnea Syndromes; Specialist; System; Technical Expertise; Tissue Sample; Western Blotting; Work; abstracting; brain cell; cost; insight; instructor; interest; kit protein; laser capture microdissection; professor; research study; sample collection

Abstract – Core B This core focuses on qualitative and quantitative analyses of genes, proteins, peptides and small drug compounds using conventional biochemical methods coupled with highly sensitive mass spectrometry (MS) and Laser Capture Microdissection (LCM) for sample collection. It will serve as a central resource for LCM and qRT-PCR (UNTHSC) and MS analysis of covalent protein modifications (UTHSCSA) of CNS samples from the 3 projects. The core will receive raw samples from investigators, most often these will be in the form of punches from specific brain regions of interest. LCM will be used to harvest defined populations of cells from brain sections allowing the projects to conduct experiments using samples that are more specific than punched samples. Retrograde track tracing, juxtacellular labeling, immunohistochemistry, and the expression of reporter molecules will be used to identify neurons of interest.

摘要-核心B 该核心集中于基因、蛋白质、多肽和小分子药物的定性和定量分析 使用常规生物化学方法结合高灵敏度质谱（MS）测定化合物 和激光捕获显微切割（LCM）用于样品收集。它将作为LCM的核心资源， 来自以下的CNS样品的共价蛋白修饰的qRT-PCR（UNTHSC）和MS分析（UTHSCSA）： 3个项目。岩心将从研究人员那里收到原始样本，这些样本通常是 从特定的大脑区域发出的信息LCM将用于从以下细胞收获确定的细胞群： 大脑部分允许项目使用比打孔更具体的样本进行实验 样品逆行追踪、细胞标记、免疫组织化学和报告基因的表达 分子将被用于识别感兴趣的神经元。