课题基金 / 基金详情

Integrase Binding Proteins as Drug Targets to Inhibit HIV-1 Assembly

Integrase Binding Proteins as Drug Targets to Inhibit HIV-1 Assembly
整合酶结合蛋白作为抑制 HIV-1 组装的药物靶点
批准号:
9072152
负责人:
GANJAM V KALPANA
金额:
$16.7万
依托单位国家:
美国
项目类别:
财政年份:
2014
资助国家:
美国
项目状态:
已结题
起止时间:
2014-09-01 至 2018-08-31

项目摘要

项目成果

GANJAM V KALPANA的其他基金

相似基金

相关文献

中文摘要
翻译

英文摘要
 DESCRIPTION (provided by applicant): This application is a revision to the awarded R01 "1R01GM112520-01, Integrase Binding Proteins as Drug Targets to Inhibit HIV-1 Assembly." The reason for this revision is the availability of new NMR based solution structure for INI1. Availability of NMR structure enhances our ability to perform the goals of funded R01, whose Aims are as follows. The Aim I is to understand the mechanism by which INI1 influences assembly; Aim II is to understand the nuclear export properties of INI1; and the Aim III is to: (i) define the interaction of minimal IN binding domain of INI1 with IN using computational modeling and identify the interface residues involved in IN-INI1 interactions; and (ii) to screen for drugs/peptidomimetics that disrupt IN-INI1 interaction using Alpha Screen. However, NMR based structural determination of INI1 or IN-INI1 interactions are not part of the goals of the R01. The newly proposed supplemental studies will enhance our ability to perform the goals of Aims I and III, as described below. INI1 is an integrase binding protein that has been shown to influence multiple stages of HIV-1 replication including LTR-mediated transcription, assembly and particle production and integration. In addition, INI1 is an epigenetic regulator and a component of SWI/SNF complex involved in chromatin remodeling. It is a tumor suppressor mutated in large number of human cancers. Despite its wide spread importance in cancer, epigenetic and transcriptional regulation and HIV-1 replication, the structure of INI1 is unknown, largely due to inability to obtain large quantities of pure protein or inability to crystallize. W have been recently successful in purifying and obtaining NMR structure of the transdominant negative mutant of INI1, also termed, S6(Rpt1) or INI1183-265. Having solution structure of INI1183-265 rapidly enhances our ability to understand the structural basis of IN-INI1 interactions and enriches our ability to attain the goals proposed in the aims of R01, especially aim III. With INI1183-265 structure in hand, the goals for the supplemental application are as follows. In Supplemental Aim I we will use NMR-based chemical shift perturbation (CSP) mapping to identify the interface residues within IN-INI1 complexes and use standard NMR methods to determine the complete structure of the INI1183-265 - IN complex. In Supplemental Aim II, we will carry out structure-based functional studies by generating mutations of interface residues of IN and INI1 and test the effect of these mutants on HIV-1 replication, especially assembly and particle production. These studies will allow, for the first time, to gain understanding of the structural basis of IN-INI1 interactions, their effect on HIV-1 replication, and pave the way for drug development to disrupt these interactions.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
RNA-mimicry to guide the intra-cellular targeting of host virus protein and viral RNA-protein interactions to inhibit HIV replication.
RNA-mimicry to guide the intra-cellular targeting of host virus protein and viral RNA-protein interactions to inhibit HIV replication.
Single cell RNA-seq and single molecule RNA-FISH approaches to study stochasticity of latent HIV-1 reactivation
Structure-based design of stapled peptides to target Gag-Pol and INI1 interaction to block assembly
海外基金