Core B: Molecular Detection and Stereology Core

分子检测和体视学核心（MDSC）为UAB神经科学社区提供成本- 免疫组织化学（IHC）和原位杂交（ISH）各方面的有效培训和专业知识 应用于现代神经科学研究的程序。近十年来，核心一直专注于 培训技术人员，本科生和研究生，博士后研究员和教师调查员， IHC和ISH的理论与实践。这种对教育和实践培训的重视促进了 这些技术的传播超出了核心本身，并允许将IHC和ISH整合到许多 NINDS资助了UAB的实验室。对于有经验的用户，Core可直接协助进行故障排除 困难的抗体，建立先进的检测放大技术，如酪胺信号 扩增和量子点，并开发多标记检测程序。在这次竞争激烈的续约中 根据申请，大卫·斯坦德特博士担任核心B的主要研究者。他是个临床医生- 科学家长期致力于研究和实验的优秀背景 神经解剖学Terry刘易斯博士仍然是核心的技术总监，拥有超过十年的经验 神经科学相关的研究。刘易斯博士和玛丽莎梅纳尔女士，一位才华横溢的实验室技术员， 在整个培训和实验研究过程中，与核心用户密切和直接联系。提出要 继续提供这些服务。 建议MDSC提供微视场体视学/形态测量系统，其进一步 使UAB神经科学家能够准确评估和量化其MHC和ISH的结果 调查事务所MDSC将提供的额外体视学服务使核心处于独特的地位， 培训并协助UAB神经科学研究者完成IHC和ISH的计划和实施 从组织固定、切片制备、IHC/ISH方案优化和最终分析的实验 使用Microtechnology Field系统。 在过去九年的资助中，MDSC已被证明对NINDS资助的服务很好。 研究人员和整个UAB神经科学界。核心一贯训练和协助超过50 学生，博士后和研究人员每年，并被广泛认为是“去”的地方IHC，ISH和 体视学专业知识。在目前申请的19个合资格赠款中，有16个已使用MDSC 强调核心的生产力和对其服务的明确持续需求。通过提供这些服务， MDSC减少了实验室的启动时间和多余的资源， 体视学方法纳入他们的研究，以及提高生产力的更有经验的研究人员， 扩大其能力，以实施最近开发的和强大的新的敏感检测方法。

The Molecular Detection and Stereology Core (MDSC) provides the UAB Neuroscience community with cost- effective training and expertise in all aspects of immunohistochemistry (IHC) and in situ hybridization (ISH) procedures as applied to modern neuroscience research. For nearly ten years, the Core has focused on training technicians, undergraduate and graduate students, post-doctoral fellows, and faculty investigators in the theory and practice of IHC and ISH. This emphasis on education and hands on training facilitates the propagation of these techniques beyond the Core itself and allows integration of IHC and ISH into many of the NINDS funded laboratories at UAB. For experienced users, the Core directly assists with troubleshooting difficult antibodies, establishing advanced detection amplification techniques, such as tyramide signal amplification and Quantum Dots, and developing multi-label detection procedures. In this competitive renewal application, Dr. David Standaert has assumed the role of Principal Investigator of Core B. He is a clinician- scientist with a longstanding commitment to research and an excellent background in experimental neuroanatomy. Dr. Terry Lewis remains the Technical Director of the Core and has over ten years' experience in neuroscience related research. Dr. Lewis and Ms. Marissa Menard, a talented laboratory technician, work closely and directly with Core users throughout their training and experimental investigations. It is proposed to continue these services. It is proposed that the MDSC provide MicroBrightField stereology/morphometric systems which further enables UAB neuroscientists to accurately assess and quantitate the results of their IHC and ISH investigations. The additional stereology services to be offered in the MDSC uniquely positions the Core to train and assist UAB Neuroscience investigators with complete planning and performance of IHC and ISH experiments from fixation of tissue, section preparation, IHC/ISH protocol optimization, and final analysis utilizing the MicroBrightField systems. Over the previous nine years of funding, the MDSC has proven to be of great service to NINDS funded investigators and the entire UAB Neuroscience community. The Core consistently trains and assists over 50 students, postdocs, and investigators per year and is widely recognized as the “go to” place for IHC, ISH and stereology expertise.. Of the 19 qualifying grants in the current application, 16 have utilized the MDSC emphasizing the Core's productivity and clear ongoing need for its services. By providing these services, the MDSC reduces startup time and redundant resources for laboratories wishing to incorporate IHC, ISH and stereology methods into their research as well as increasing productivity of more experienced investigators by expanding their capabilities to implement recently developed and powerful new sensitive detection methods.