The Influence of Neurotransmitter Transport on Aminergic Neuromodulation
The Influence of Neurotransmitter Transport on Aminergic Neuromodulation
批准号:
9059776
负责人:
David Evan Krantz
金额:
$38.5万
依托单位国家:
美国
项目类别:
财政年份:
2015
资助国家:
美国
项目状态:
已结题
起止时间:
2015-05-01 至 2020-01-31
关键词:
AddressAdultAffectAggressive behaviorAminesAnatomyAnimal ModelAntidepressive AgentsAttentionBehaviorBehavioralBiologicalBiological AssayCell membraneCellsChimera organismComplementComplexDataDense Core VesicleDopamineDrosophila genusDrosophila melanogasterElementsGeneticHealthIndividualInvertebratesLogicMammalsMapsMeasurementMediatingMembrane Transport ProteinsMental DepressionModelingMolecularMoodsMutationNerveNeuromuscular JunctionNeuronsNeurotransmittersNorepinephrineOdorsPHluorinPharmaceutical PreparationsProcessProteinsRegulationSecretory VesiclesSerotoninSignal TransductionSiteSleepSorting - Cell MovementSynapsesSynaptic TransmissionSynaptic VesiclesTestingTimeTranscriptional RegulationVesiclebehavior influencebehavior testbehavioral responseclinical effecteggextracellularflyin vivoinnovationinterestmonoaminemutantneuroregulationneurotransmitter releaseneurotransmitter transportneurotransmitter uptakenovelpresynapticreceptorresearch studyresponsesynaptic functiontooltraffickingvesicular monoamine transportervesicular releasevisual tracking
中文摘要
英文摘要
DESCRIPTION (provided by applicant): Vesicular monoamine transporters (VMATs) are required for the storage and exocytotic release of all aminergic neurotransmitters. The mechanisms by which VMATs target to secretory vesicles remain unclear and the potential behavioral consequences of disrupting their localization are unknown. We are using the model organism Drosophila melanogaster to address these questions. The mutations we have generated thus far decrease the localization of Drosophila VMAT (DVMAT) to Synaptic Vesicles (SVs) and increase its localization to Large Dense Core Vesicles (LDCVs). The behavioral sequelae of these mutations provide some of the first information on the function of amine release from SVs versus LDCVs. We will now generate additional mutations to decrease the localization of DVMAT to LDCVs. These mutants will be useful for further behavioral tests and also help to define fundamental trafficking mechanisms in neurons. Additional experiments will use these mutants to define the poorly understood differences between trafficking in aminergic versus non-aminergic neurons and to determine how changes in amine release affect pre- and post-synaptic function in aminergic circuits. Further behavioral experiments will explore the affects of altered amine release in more complex behaviors and the response to aminergic drugs. The results of these experiments will be significant because they examine processes relevant to conserved neuromodulatory processes and the clinical effects of aminergic drugs. They are innovative because they exploit several new assays and because no other lab has examined the in vivo effects of mis-trafficking for a vesicular transporter, or the behavioral effects of changing the way neurotransmitters are released from particular vesicle types.
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会议论文
Models of Neuromodulatory Circuits in Drosophila
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批准号:10437744
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项目类别:
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资助金额:$41.07万
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财政年份:2018
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负责人:David Evan Krantz
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依托单位:
Models of Neuromodulatory Circuits in Drosophila
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批准号:10201438
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项目类别:
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资助金额:$41.07万
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财政年份:2018
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负责人:David Evan Krantz
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依托单位:
Identification of the Kenyon Cell Neurotransmitter
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批准号:9191360
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项目类别:
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资助金额:$19.25万
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财政年份:2016
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负责人:David Evan Krantz
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依托单位:
Project 2: Pesticide Mechanisms and PD: Genetic Studies In Flies
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批准号:8292135
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项目类别:
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资助金额:$31.67万
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财政年份:2011
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负责人:David Evan Krantz
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依托单位:
Environmental toxin interactions with genetic risks for Parkinson's disease
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批准号:7911174
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项目类别:
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资助金额:$30.65万
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财政年份:2009
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负责人:David Evan Krantz
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依托单位:
Environmental toxin interactions with genetic risks for Parkinson's disease
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批准号:7818366
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项目类别:
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资助金额:$19.23万
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财政年份:2009
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负责人:David Evan Krantz
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依托单位:
Trafficking of the Drosophila Vesicular Monoamine Transporter
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批准号:8068853
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项目类别:
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资助金额:$30.24万
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财政年份:2007
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负责人:David Evan Krantz
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依托单位:
Trafficking of the Drosophila Vesicular Monoamine Transporter
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批准号:7317390
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项目类别:
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资助金额:$31.89万
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财政年份:2007
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负责人:David Evan Krantz
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依托单位:
Trafficking of the Drosophila Vesicular Monoamine Transporter
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批准号:7808800
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项目类别:
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资助金额:$30.54万
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财政年份:2007
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负责人:David Evan Krantz
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依托单位:
Environmental toxin interactions with genetic risks for Parkinson's disease
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批准号:8232880
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项目类别:
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资助金额:$15.0万
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财政年份:2007
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负责人:David Evan Krantz
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依托单位:
Environmental toxin interactions with genetic risks for Parkinson's disease
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批准号:7289933
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项目类别:
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资助金额:$36.58万
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财政年份:2007
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负责人:David Evan Krantz
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依托单位:
Environmental toxin interactions with genetic risks for Parkinson's disease
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批准号:7624657
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项目类别:
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资助金额:$35.84万
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财政年份:2007
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负责人:David Evan Krantz
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依托单位:
Trafficking of the Drosophila Vesicular Monoamine Transporter
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批准号:7618462
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项目类别:
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资助金额:$30.54万
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财政年份:2007
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负责人:David Evan Krantz
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依托单位:
Trafficking of the Drosophila Vesicular Monoamine Transporter
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批准号:7477960
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项目类别:
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资助金额:$30.54万
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财政年份:2007
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负责人:David Evan Krantz
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依托单位:
Environmental toxin interactions with genetic risks for Parkinson's disease
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批准号:7477104
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项目类别:
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资助金额:$35.84万
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财政年份:2007
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负责人:David Evan Krantz
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依托单位:
VESICULAR NEUROTRANSMITTER TRANSPORT IN DROSOPHILA
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批准号:6440330
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项目类别:
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资助金额:$14.65万
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财政年份:2001
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负责人:David Evan Krantz
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依托单位:
REGULATION OF VESICULAR NEUROTRANSMITTER TRANSPORT IN D*
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批准号:6524656
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项目类别:
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资助金额:$15.25万
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财政年份:2001
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负责人:David Evan Krantz
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依托单位:
REGULATION OF VESICULAR NEUROTRANSMITTER TRANSPORT
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批准号:6185454
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项目类别:
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资助金额:$3.72万
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财政年份:1999
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负责人:David Evan Krantz
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依托单位:
REGULATION OF VESICULAR NEUROTRANSMITTER TRANSPORT
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批准号:6369401
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项目类别:
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资助金额:$3.56万
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财政年份:1999
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负责人:David Evan Krantz
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依托单位:
REGULATION OF VESICULAR NEUROTRANSMITTER TRANSPORT
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批准号:2859374
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项目类别:
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资助金额:$6.74万
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财政年份:1999
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负责人:David Evan Krantz
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依托单位:
海外基金