Research Supplement to promote diversity in health-related research

描述（由申请人提供）：该提案将测试小细胞激酶6 （KLK6）蛋白在功能上参与结肠癌发生的假设，并可能是结肠癌发生的有用标记物。结果表明：1)在小鼠结肠腺瘤和人结肠肿瘤中均可检测到KLK6蛋白；2) KLK6功能阻断抗体可减弱结肠癌细胞的侵袭；3)敲低KLK6的结肠癌细胞在SCID小鼠模型中不形成远处转移，提高了动物存活率。为了验证整体假设，我们提出以下具体目标：1)确定KLK6在体内结肠癌进展中的作用；2)确定KLK6在结肠癌转移中的作用；3)评估结肠中KLK6蛋白表达和酶活性。本提案的主要目的是确定KLK6在结肠肿瘤和转移形成中的作用，开发一种测量结肠中KLK6酶活性的成像模式，并将其与疾病进展联系起来。

DESCRIPTION (provided by applicant): This proposal will test the hypothesis that kallikrein 6 (KLK6) protein is functionally involved in, and may be a useful marker of, colon carcinogenesis. We have shown that: 1) KLK6 protein can be detected at the adenomas in the mouse colon, and in human colon tumors; 2) KLK6 function blocking antibody attenuates invasion of colon cancer cells; and 3) colon cancer cells with knockdown of KLK6 do not form distant metastases in SCID mouse model and increase animal survival rates. To test the overall hypothesis, we propose the following specific aims: 1) determine the role of KLK6 in the colon cancer progression in vivo; 2) determine the role of KLK6 in colon cancer metastasis; 3) evaluate KLK6 protein expression and enzymatic activity in the colon. The major objectives of this proposal are to determine the role of KLK6 in colon tumor and metastasis formation, develop an imaging modality for measuring of KLK6 enzymatic activity in the colon and correlate it with disease progression.