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Experimental Mouse

实验小鼠

基本信息

批准号：
10407089
负责人：
NATALIA A IGNATENKO
金额：
$ 12.84万
依托单位：
UNIVERSITY OF ARIZONA
依托单位国家：
美国
项目类别：
财政年份：
1997
资助国家：
美国
起止时间：
1997-07-01 至 2022-06-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/10407089
关键词：
Advocacy Animals Arizona Cancer Center Cancer Center Support Grant Cancer Model Cancer Research Project Communities Cryopreservation Custom Data Devices Ensure Equipment Ethical Issues Experimental Designs Generations Genetically Engineered Mouse Goals Human Resources Immunocompromised Host Institution Intervention Laboratories Malignant Neoplasms Microscopic Modeling Monitor Mouse Strains Mus Operative Surgical Procedures Organ Pathologic Procedures Process Production Productivity Protocols documentation Quality Control Rattus Reagent Reproducibility Research Personnel Resource Sharing Resources Rodent Rodent Model Services Techniques Technology Testing Time Tissues Training Training and Education Universities Vibrissae Work design equipment training implantation in vivo in vivo Model innovation interest member mouse Cre recombinase mouse model novel patient derived xenograft model precision medicine tissue preparation tool tumor tumor growth

项目摘要

Project Summary / Abstract: Experimental Mouse Shared Resource (EMSR) The goal of the Experimental Mouse Shared Resource (EMSR) is to enhance the productivity and capabilities of Members of the University of Arizona Cancer Center (UACC) by providing access to in vivo models, including immunocompromised and genetically engineered mouse (GEM) models, and to provide comprehensive support of experimentation in rodents (mouse and rat). The EMSR is dedicated to effective and efficient service that allows UACC Members to avoid duplication of equipment and training that would occur without such a Resource and to ensure the reproducibility of technical interventions and provide the rigorous quality control required for informative analyses of animal studies. EMSR personnel process tissues and perform procedures for experimental endpoints, including gross pathologic examinations with cryopreservation of organs/tissues and preparation of tissues for microscopic examination for further analysis by other UACC Shared Resources. The EMSR is divided into two sections: 1) the GEM Production Unit which utilizes the University of Arizona's Genetically Engineered Mouse Modeling (GEMM) core to generate the GEMs through “sequence-to-whiskers” services, and 2) the Rodent Experimentation Unit which provides “whiskers-to-data” services. These two units are complimentary and interactive, sharing animals, techniques, equipment and reagents. The EMSR maintains two blanket protocols for GEM generation and pilot work. The Unit is in compliance with the University's Biosafety Committee rules and is GLP-certified.

项目摘要/摘要：实验鼠标共享资源 (EMSR) 实验鼠标共享资源 (EMSR) 的目标是提高生产力和能力亚利桑那大学癌症中心 (UACC) 的成员通过提供体内模型，包括免疫受损和基因工程小鼠 (GEM) 模型，并提供啮齿动物（小鼠和大鼠）实验的全面支持。 EMSR 致力于有效且高效的服务使 UACC 会员能够避免可能发生的设备和培训重复没有这样的资源并确保技术干预的可重复性并提供严格的动物研究信息分析所需的质量控制。 EMSR 人员处理组织和执行实验终点程序，包括冷冻保存的大体病理检查器官/组织的制备以及用于显微镜检查的组织的制备，以供其他 UACC 进一步分析共享资源。 EMSR 分为两个部分：1) GEM 生产单元，利用亚利桑那大学的基因工程小鼠模型 (GEMM) 核心通过以下方式生成 GEM： “序列到晶须”服务，以及 2) 提供“晶须到数据”的啮齿动物实验单元服务。这两个单元是互补和互动的，分享动物、技术、设备和试剂。 EMSR 维护着两个用于 GEM 生成和试点工作的总协议。该单位位于符合大学生物安全委员会的规定，并通过了 GLP 认证。