Experimental Mouse

项目摘要/摘要：实验小鼠共享资源(EMSR) 实验小鼠共享资源(EMSR)的目标是提高生产力和能力 通过提供体内模型，亚利桑那大学癌症中心(UACC)的成员， 包括免疫受损和基因工程小鼠(GEM)模型，并提供 对啮齿动物(小鼠和大鼠)实验的全面支持。EMSR致力于有效和 高效的服务，使UACC成员避免可能发生的重复设备和培训 没有这样的资源，并确保技术干预的可重复性，并提供严格的 动物研究的信息分析所需的质量控制。EMSR人员处理组织和 对实验终点进行程序，包括冷冻保存的大体病理检查 器官/组织和准备用于显微镜检查的组织，以供其他UACC进一步分析 共享资源。EMSR分为两个部分：1)宝石生产单位，利用 亚利桑那大学的基因工程老鼠建模(GEMM)核心通过 “序列到胡须”服务，以及2)提供“胡须到数据”的啮齿动物实验股。 服务。这两个单元是互补和互动的，共享动物、技术、设备和 试剂。EMSR为创业板的生成和试点工作维护了两个一揽子协议。该单位在 遵守大学生物安全委员会的规定，并获得GLP认证。

Project Summary / Abstract: Experimental Mouse Shared Resource (EMSR) The goal of the Experimental Mouse Shared Resource (EMSR) is to enhance the productivity and capabilities of Members of the University of Arizona Cancer Center (UACC) by providing access to in vivo models, including immunocompromised and genetically engineered mouse (GEM) models, and to provide comprehensive support of experimentation in rodents (mouse and rat). The EMSR is dedicated to effective and efficient service that allows UACC Members to avoid duplication of equipment and training that would occur without such a Resource and to ensure the reproducibility of technical interventions and provide the rigorous quality control required for informative analyses of animal studies. EMSR personnel process tissues and perform procedures for experimental endpoints, including gross pathologic examinations with cryopreservation of organs/tissues and preparation of tissues for microscopic examination for further analysis by other UACC Shared Resources. The EMSR is divided into two sections: 1) the GEM Production Unit which utilizes the University of Arizona's Genetically Engineered Mouse Modeling (GEMM) core to generate the GEMs through “sequence-to-whiskers” services, and 2) the Rodent Experimentation Unit which provides “whiskers-to-data” services. These two units are complimentary and interactive, sharing animals, techniques, equipment and reagents. The EMSR maintains two blanket protocols for GEM generation and pilot work. The Unit is in compliance with the University's Biosafety Committee rules and is GLP-certified.