Improvement of genetic analysis in the pathogenic zygomycete Mucor circinelloides

 描述(由申请人提供)：本提案旨在开发合菌类真菌卷曲毛霉的增强遗传分析系统。盘状分枝杆菌是一种能引起毛霉病的机会性真菌病原体。由于免疫缺陷个体数量的增加，毛霉病的发病率正在增加。环状微孢子虫也是研究光感应和理解RNAi介导的基因表达调控的模型系统。此外，卷曲霉菌也是生产生物燃料的候选菌株。然而，由于1)用于转化的标记数量有限，以及2)转化效率低和同源基因替换频率低，该真菌系统的遗传分析受到限制。在本文件提出的研究中，我们旨在通过提出三个目标来提高我们在这一重要真菌系统中的遗传分析能力：1)构建可回收的PYRG标记系统；2)促进基因中断中的同源重组。

 DESCRIPTION (provided by applicant): This proposal aims to develop enhanced genetic analysis systems in the zygomycete fungus Mucor circinelloides. M. circinelloides is an opportunistic fungal pathogen that causes mucormycosis. The incidence of mucormycosis is increasing due to a rising number of immunocompromised individuals. M. circinelloides also serves as a model system to study light sensing and understand RNAi-mediated gene expression regulation. In addition, M. circinelloides is a candidate for biofuel production. However, the genetic analysis of this fungal system is limited due to 1) a limited number of markers for transformation and 2) inefficient transformation and low frequent homologous gene replacement. In the studies proposed within this document, we aim to enhance our ability for genetic analysis in this important fungal system by proposing three aims: 1) construction of a recyclable pyrG marker system and 2) enhancement of homologous recombination in gene disruptions.