Improvement of genetic analysis in the pathogenic zygomycete Mucor circinelloides

致病性接合菌卷枝毛霉遗传分析的改进

• 批准号: 9109242
• 负责人: Soo Chan Lee
• 金额: $ 3.63万
• 依托单位: DUKE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-02-01 至 2016-08-01
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9109242
• 关键词: Acquired Immunodeficiency Syndrome; Affect; Biological Models; Caring; Class Zygomycetes; Communicable Diseases; Complex; DNA Integration; Development; Diabetes Mellitus; Drug resistance; Event; Excision; Exhibits; Foundations; G22P1 gene; Gene Expression Regulation; Genes; Genetic; Genetic Recombination; Genome; Grant; Hematologic Neoplasms; Hematopoietic stem cells; Homologous Gene; Human; Immunocompromised Host; Incidence; Individual; Infection; Leucine; Light; Mediating; Medical; Modeling; Molds; Molecular Biology; Molecular Genetics; Mucor; Mucorales; Mucormycosis; Mycoses; Organ Transplantation; Orthologous Gene; PPP3CB gene; Patients; Phenotype; Production; RNA Interference; Research Methodology; Route; Solid; System; Trauma; Uridine; Work; XRCC5 gene; Yeasts; aging population; fungus; gene function; gene replacement; genetic analysis; genome sequencing; homologous recombination; mutant; novel; pathogen; public health relevance; research and development; tool

 DESCRIPTION (provided by applicant): This proposal aims to develop enhanced genetic analysis systems in the zygomycete fungus Mucor circinelloides. M. circinelloides is an opportunistic fungal pathogen that causes mucormycosis. The incidence of mucormycosis is increasing due to a rising number of immunocompromised individuals. M. circinelloides also serves as a model system to study light sensing and understand RNAi-mediated gene expression regulation. In addition, M. circinelloides is a candidate for biofuel production. However, the genetic analysis of this fungal system is limited due to 1) a limited number of markers for transformation and 2) inefficient transformation and low frequent homologous gene replacement. In the studies proposed within this document, we aim to enhance our ability for genetic analysis in this important fungal system by proposing three aims: 1) construction of a recyclable pyrG marker system and 2) enhancement of homologous recombination in gene disruptions.

 描述（由申请人提供）：该提案旨在开发在放线菌真菌卷枝毛霉中增强的遗传分析系统。M.卷枝霉是一种引起毛霉病的机会性真菌病原体。毛霉菌病的发病率正在增加，由于越来越多的免疫功能低下的个人。M. circinelloides也作为一个模型系统，研究光传感和理解RNAi介导的基因表达调控。此外，M. circinelloides是生物燃料生产的候选者。然而，该真菌系统的遗传分析是有限的，这是由于1）用于转化的标记数量有限和2）转化效率低和同源基因替换频率低。在本文中提出的研究中，我们的目标是通过提出三个目标来增强我们在这个重要的真菌系统中进行遗传分析的能力：1）构建可回收的pyrG标记系统和2）增强基因破坏中的同源重组。