Improvement of genetic analysis in the pathogenic zygomycete Mucor circinelloides
Improvement of genetic analysis in the pathogenic zygomycete Mucor circinelloides
批准号:
9109242
负责人:
Soo Chan Lee
金额:
$3.63万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2016
资助国家:
美国
项目状态:
已结题
起止时间:
2016-02-01 至 2016-08-01
关键词:
Acquired Immunodeficiency SyndromeAffectBiological ModelsCaringClass ZygomycetesCommunicable DiseasesComplexDNA IntegrationDevelopmentDiabetes MellitusDrug resistanceEventExcisionExhibitsFoundationsG22P1 geneGene Expression RegulationGenesGeneticGenetic RecombinationGenomeGrantHematologic NeoplasmsHematopoietic stem cellsHomologous GeneHumanImmunocompromised HostIncidenceIndividualInfectionLeucineLightMediatingMedicalModelingMoldsMolecular BiologyMolecular GeneticsMucorMucoralesMucormycosisMycosesOrgan TransplantationOrthologous GenePPP3CB genePatientsPhenotypeProductionRNA InterferenceResearch MethodologyRouteSolidSystemTraumaUridineWorkXRCC5 geneYeastsaging populationfungusgene functiongene replacementgenetic analysisgenome sequencinghomologous recombinationmutantnovelpathogenpublic health relevanceresearch and developmenttool
中文摘要
英文摘要
DESCRIPTION (provided by applicant): This proposal aims to develop enhanced genetic analysis systems in the zygomycete fungus Mucor circinelloides. M. circinelloides is an opportunistic fungal pathogen that causes mucormycosis. The incidence of mucormycosis is increasing due to a rising number of immunocompromised individuals. M. circinelloides also serves as a model system to study light sensing and understand RNAi-mediated gene expression regulation. In addition, M. circinelloides is a candidate for biofuel production. However, the genetic analysis of this fungal system is limited due to 1) a limited number of markers for transformation and 2) inefficient transformation and low frequent homologous gene replacement. In the studies proposed within this document, we aim to enhance our ability for genetic analysis in this important fungal system by proposing three aims: 1) construction of a recyclable pyrG marker system and 2) enhancement of homologous recombination in gene disruptions.
期刊论文(0)
专著(0)
科研奖励(0)
会议论文
18th South Central Medical Mycology Annual Conference, 2021
-
批准号:10391234
-
项目类别:
-
资助金额:$0.8万
-
财政年份:2022
-
负责人:Soo Chan Lee
-
依托单位:
Improvement of genetic analysis in the pathogenic zygomycete Mucor circinelloides
-
批准号:9388875
-
项目类别:
-
资助金额:$4.0万
-
财政年份:2016
-
负责人:Soo Chan Lee
-
依托单位:
海外基金