Induced Pluripotent Stem Cell Core for NiNDS Investigators

描述：将体细胞重新编程为多能干细胞(诱导多能干细胞-IPSCs)的能力改变了人类基础细胞神经生物学的研究和人类神经系统疾病的细胞基础的研究。这项技术使研究人类神经干细胞、大量人类神经元表型以及人类星形胶质细胞和少突胶质细胞的发育和细胞生物学成为可能。此外，来自各种神经系统疾病患者的体细胞可以重新编程，以便根据患者的基因类型研究神经元和神经胶质细胞。毫无疑问，从这类研究中获得的见解将转化为更好的治疗方法，并最终治愈神经疾病。这项提议的战略目标是将这项关键技术交到NU的一群高生产率的神经科学家手中，他们目前从NINDS获得了近600万美元的资金(直接成本)。具体地说，该中心将：1.提供设施和专业知识，使NINDS研究人员能够培养和分化人类IPSCs，并使他们能够利用科里尔研究所和其他地方的细胞库。NINDS研究人员将根据需要创建新的IPSC系，这些系将保存在科里尔研究所2的NINDS人类细胞系储存库中。提供设施和专业知识来确定分化的IPSC的表型，包括IPSC来源的神经元和神经胶质的形态、电和其他特性。3.使NINDS研究人员能够在他们的研究方案中利用NU开发的新的纳米技术进步来创造和分析IPSC。这些目标的实现不仅将对NINDS资助的NINDS研究项目产生革命性的影响，并利用NINDS的投资，而且还将显著加快将这些技术交付给更广泛的神经科学界，加快科学发现的步伐，并促进神经疾病新疗法的开发。

DESCRIPTION: The ability to reprogram somatic cells into pluripotent stem cells (induced pluripotent stem cells - iPSCs) has transformed both the study of basic human cellular neurobiology and the examination of the cellular basis of human diseases of the nervous system. This technology makes it possible to study the development and cell biology of human neural stem cells, numerous human neuronal phenotypes, and human astrocytes and oligodendroglia. Further, somatic cells from patients with a variety of neurological diseases can be reprogrammed to allow study of neurons and glia with the genotypes of the afflicted individuals. There is no doubt that insights gained from such studies will translate into better treatments and eventually cures for neurological diseases. The strategic goal of this proposal is to put this critical technology into the hands of a highly productive group of neuroscientists at NU that currently receive nearly $6 million (direct costs) in funding from NINDS. Specifically the Center will: 1. Provide the facilities and expertise to enable NINDS investigators to culture and differentiate human iPSCs and to enable them to take advantage of cell repositories at the Coriell Institute and elsewhere. New iPSC lines will be created as needed for programs of NINDS investigators, and these lines will be deposited in the NINDS human cell line repository at the Coriell Institute 2. Provide the facilities and expertise to define the phenotype of differentiated iPSCs including morphology, electrical and other properties of iPSC-derived neurons and glia. 3. Enable NINDS investigators to utilize in their research programs new nanotechnology advances developed at NU for the creation and analysis of iPSCs. The attainment of these goals will not only have a transforming impact on the NINDS-funded research programs at NU and leverage the NINDS investment, but will also significantly accelerate the delivery of these technologies to the broader neuroscience community, quicken the pace of scientific discovery and promote the development of new treatments for neurological disorders.