Metal-oxidative stress interplay in periodontopathogen Prevotella intermedia
Metal-oxidative stress interplay in periodontopathogen Prevotella intermedia
批准号:
9194618
负责人:
Janina P Lewis
金额:
$18.91万
依托单位国家:
美国
项目类别:
财政年份:
2016
资助国家:
美国
项目状态:
已结题
起止时间:
2016-08-01 至 2018-07-31
关键词:
AerobicAerobic BacteriaAlphaproteobacteriaAnaerobic BacteriaAnti-Bacterial AgentsBacteriaBacteroidetesBindingBiochemicalBiologicalBiological AssayChIP-seqDataDevelopmentEmployee StrikesEnvironmentEscherichia coliFutureGene ExpressionGene TargetingGenerationsHeminHomeostasisHomologous GeneImmune responseInfectionInvestigationIronLeadMetalsMethodsOralOxidative StressOxygenPeriodontal DiseasesPlayPorphyromonasPorphyromonas gingivalisPreventive measurePrevotella intermediaProteomicsRegulationRegulonRoleSamplingTherapeutic AgentsVirulence FactorsWorkbasedesignenvironmental changegenetic regulatory proteingenomic profilesin vivomicrobiomenovelpathogenperiodontopathogenprotein expressiontranscriptome sequencinguptake
中文摘要
铁氧相互作用是建立感染的必要条件,包括能力
英文摘要
Iron-oxygen interplay is essential in establishment of an infection that includes the ability
of the pathogen to escape host response and multiply within its host. Although it differs
significantly in anaerobes, that include periodontopathogens such as Porphyromonas
gingivalis and Prevotella intermedia, when compared to aerobes little is known as
regards the iron homeostasis in anaerobic bacteria. We have shown that many putative
iron acquisition mechanisms are regulated by iron in both Porphyromonas gingivalis and
Prevotella intermedia, however, our data also show that the orthologue of the ferric
uptake regulator, Fur, is not the major iron homeostasis regulator in P. gingivalis pointing
out that novel mechanism of iron regulation must be present in this bacterium.
Furthermore, the P. gingivalis oxidative stress regulator, OxyR, is involved in hemin
homeostasis in this bacterium. Based on such striking differences in iron-dependent
regulation we hypothesize that the differences extend beyond the genus Porphyromonas
and include other anaerobic bacteria belonging to the phylum Bacteroidetes. Here we
propose to extend our investigations by characterizing the role of the P. intermedia
orthologue of a ferric uptake regulator, Fur, in iron homeostasis in this bacterium.
Furthermore, as iron plays crucial role in generation of oxidative stress species we will
also examine the role of a P. intermedia homologue of the oxidative stress regulator,
OxyR. First, we will define the biological role of the two regulators in P. intermedia using
biological assays as well as genomic profiling of both gene and protein expression. Next,
we will define the regulons of both Fur and OxyR in P. intermedia. As P. intermedia is
anaerobic bacterium and is distantly related to the well known aerobic, alpha
proteobacteria such as Escherichia coli, we hypothesize novel findings will be gained
from this investigation. The results of our study will help to define iron and oxidative
stress homeostasis mechanisms that in turn may serve as targets for the future design
of antibacterial agents for P. intermedia. Since P. intermedia is phylogenetically similar
to many other anaerobic medically significant bacteria such information will also be
applied in development of an alternative methods of preventive measures for other
bacteria.
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会议论文
Bioinformatics analysis of host-microbiome interaction in oral cavity
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批准号:10284591
-
项目类别:
-
资助金额:$14.36万
-
财政年份:2021
-
负责人:Janina P Lewis
-
依托单位:
Riboregulation in periodontopathogen Porphyromonas gingivalis
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批准号:8885795
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项目类别:
-
资助金额:$19.06万
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财政年份:2014
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负责人:Janina P Lewis
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依托单位:
Riboregulation in periodontopathogen Porphyromonas gingivalis
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批准号:8781820
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项目类别:
-
资助金额:$22.88万
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财政年份:2014
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负责人:Janina P Lewis
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依托单位:
Nitrosative Stress and Oral Bacteria
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批准号:10440244
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项目类别:
-
资助金额:$36.12万
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财政年份:2013
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负责人:Janina P Lewis
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依托单位:
Nitrosative stress defenses in periodontopathogen Porphyromonas gingivalis
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批准号:8549467
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项目类别:
-
资助金额:$38.11万
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财政年份:2013
-
负责人:Janina P Lewis
-
依托单位:
Nitrosative stress defenses in periodontopathogen Porphyromonas gingivalis
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批准号:8690018
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项目类别:
-
资助金额:$38.13万
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财政年份:2013
-
负责人:Janina P Lewis
-
依托单位:
Nitrosative Stress and Oral Bacteria
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批准号:10683718
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项目类别:
-
资助金额:$36.48万
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财政年份:2013
-
负责人:Janina P Lewis
-
依托单位:
Nitrosative stress defenses in periodontopathogen Porphyromonas gingivalis
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批准号:9057873
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项目类别:
-
资助金额:$38.13万
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财政年份:2013
-
负责人:Janina P Lewis
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依托单位:
Virulence factors of periodontopathogens
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批准号:7811931
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项目类别:
-
资助金额:$13.24万
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财政年份:2009
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负责人:Janina P Lewis
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依托单位:
Virulence factors of periodontopathogens
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批准号:8089434
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项目类别:
-
资助金额:$27.45万
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财政年份:2007
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负责人:Janina P Lewis
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依托单位:
Virulence factors of periodontopathogens
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批准号:7637403
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项目类别:
-
资助金额:$28.59万
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财政年份:2007
-
负责人:Janina P Lewis
-
依托单位:
Virulence factors of periodontopathogens
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批准号:7866685
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项目类别:
-
资助金额:$28.3万
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财政年份:2007
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负责人:Janina P Lewis
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依托单位:
Virulence factors of periodontopathogens
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批准号:7478817
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项目类别:
-
资助金额:$28.59万
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财政年份:2007
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负责人:Janina P Lewis
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依托单位:
Virulence factors of periodontopathogens
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批准号:7320420
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项目类别:
-
资助金额:$28.91万
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财政年份:2007
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负责人:Janina P Lewis
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依托单位:
Molecular Basis of Oral Microbial Adhesion
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批准号:7115845
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项目类别:
-
资助金额:$27.64万
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财政年份:2005
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负责人:Janina P Lewis
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依托单位:
Molecular Basis of Oral Microbial Adhesion
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批准号:7274170
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项目类别:
-
资助金额:$26.84万
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财政年份:2005
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负责人:Janina P Lewis
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依托单位:
Molecular Basis of Oral Microbial Adhesion
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批准号:7480294
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项目类别:
-
资助金额:$26.55万
-
财政年份:2005
-
负责人:Janina P Lewis
-
依托单位:
Molecular Basis of Oral Microbial Adhesion
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批准号:6970195
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项目类别:
-
资助金额:$28.31万
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财政年份:2005
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负责人:Janina P Lewis
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依托单位:
Prevotella intermedia: Iron and Virulence
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批准号:6760218
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项目类别:
-
资助金额:$7.5万
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财政年份:2003
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负责人:Janina P Lewis
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依托单位:
Prevotella intermedia: Iron and Virulence
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批准号:6691761
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项目类别:
-
资助金额:$7.5万
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财政年份:2003
-
负责人:Janina P Lewis
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依托单位:
海外基金