Contact-Dependent Interbacterial Signaling in Burkholderia

伯克霍尔德氏菌中接触依赖性细菌间信号传导

基本信息

  • 批准号:
    8947449
  • 负责人:
  • 金额:
    $ 16.16万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2016
  • 资助国家:
    美国
  • 起止时间:
    2016-08-01 至 2018-07-31
  • 项目状态:
    已结题

项目摘要

 DESCRIPTION (provided by applicant): Both non-pathogenic and pathogenic Burkholderia, including members of the opportunistic infection-causing Burkholderia cepacia complex, encode genes to produce contact-dependent growth inhibition (CDI) system proteins. CDI is a widespread phenomenon in which Gram-negative bacteria use the toxic C-terminus of a polymorphic surface-exposed exoprotein to inhibit the growth of susceptible bacteria upon cell-cell contact. The exoprotein toxin is delivered to the cytoplasm of target cells and autoinhibition is prevented by production of a small immunity protein. We previously demonstrated that, in addition to their role in interbacterial competition, the CDI system proteins in Burkholderia thailandensis, encoded by bcpAIOB, mediate biofilm formation. This requires the putative nuclease activity of the BcpA toxic exoprotein, but is independent of interbacterial killing, suggesting that biofilm formation in B. thailandensis involves an uncharacterized function of BcpA. Preliminary data suggest that bcpAIOB mediate interbacterial signaling in B. thailandensis, leading to a change in gene expression in immune target bacteria. Given these data and the conservation of CDI systems, it seems likely that CDI systems in other Burkholderia also function in interbacterial signaling and could play a role during infection. Toward understanding the role of CDI system-mediated signaling, this proposal will test the hypothesis that BcpA toxin delivered to an immune target cell interacts with specific periplasmic and cytoplasmic factors, leading to global gene expression changes, and represents a useful therapeutic target in pathogenic Burkholderia. Experiments proposed in Aim 1 will continue to investigate the mechanism of bcpAIOB-mediated interbacterial signaling in B. thailandensis, defining the affected gene regulation pathways and identifying required regions of BcpA, as well as other factors necessary for signaling. Studies in Aim 2 will explore the role of CDI systems and their potential for exploitation as therapies in pathogenic Burkholderia cepacia complex strains. These studies represent the first investigation of what may be a widespread phenomenon in Gram-negative bacteria and will work toward addressing an essential need for new drug targets and antimicrobials.


项目成果

期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

数据更新时间:{{ journalArticles.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ monograph.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ sciAawards.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ conferencePapers.updateTime }}

{{ item.title }}
  • 作者:
    {{ item.author }}

数据更新时间:{{ patent.updateTime }}

Erin C Garcia其他文献

Erin C Garcia的其他文献

{{ item.title }}
{{ item.translation_title }}
  • DOI:
    {{ item.doi }}
  • 发表时间:
    {{ item.publish_year }}
  • 期刊:
  • 影响因子:
    {{ item.factor }}
  • 作者:
    {{ item.authors }}
  • 通讯作者:
    {{ item.author }}

{{ truncateString('Erin C Garcia', 18)}}的其他基金

Structure and function of Burkholderia contact-dependent growth inhibition (CDI) systems
伯克霍尔德杆菌接触依赖性生长抑制(CDI)系统的结构和功能
  • 批准号:
    10265563
  • 财政年份:
    2020
  • 资助金额:
    $ 16.16万
  • 项目类别:
Structure and function of Burkholderia contact-dependent growth inhibition (CDI) systems
伯克霍尔德杆菌接触依赖性生长抑制(CDI)系统的结构和功能
  • 批准号:
    10118389
  • 财政年份:
    2020
  • 资助金额:
    $ 16.16万
  • 项目类别:
Structure and function of Burkholderia contact-dependent growth inhibition (CDI) systems
伯克霍尔德杆菌接触依赖性生长抑制(CDI)系统的结构和功能
  • 批准号:
    10687101
  • 财政年份:
    2020
  • 资助金额:
    $ 16.16万
  • 项目类别:
Structure and function of Burkholderia contact-dependent growth inhibition (CDI) systems
伯克霍尔德杆菌接触依赖性生长抑制(CDI)系统的结构和功能
  • 批准号:
    10461853
  • 财政年份:
    2020
  • 资助金额:
    $ 16.16万
  • 项目类别:
Contact-Dependent Interbacterial Signaling in Burkholderia
伯克霍尔德氏菌中接触依赖性细菌间信号传导
  • 批准号:
    9319133
  • 财政年份:
    2016
  • 资助金额:
    $ 16.16万
  • 项目类别:
Inter-Bacterial Competition Systems in Burkholderia pseudomallei
鼻疽伯克霍尔德菌的细菌间竞争系统
  • 批准号:
    8201474
  • 财政年份:
    2011
  • 资助金额:
    $ 16.16万
  • 项目类别:
Inter-Bacterial Competition Systems in Burkholderia pseudomallei
鼻疽伯克霍尔德菌的细菌间竞争系统
  • 批准号:
    8265952
  • 财政年份:
    2011
  • 资助金额:
    $ 16.16万
  • 项目类别:
Inter-Bacterial Competition Systems in Burkholderia pseudomallei
鼻疽伯克霍尔德菌的细菌间竞争系统
  • 批准号:
    8486293
  • 财政年份:
    2011
  • 资助金额:
    $ 16.16万
  • 项目类别:

相似海外基金

How Does Particle Material Properties Insoluble and Partially Soluble Affect Sensory Perception Of Fat based Products
不溶性和部分可溶的颗粒材料特性如何影响脂肪基产品的感官知觉
  • 批准号:
    BB/Z514391/1
  • 财政年份:
    2024
  • 资助金额:
    $ 16.16万
  • 项目类别:
    Training Grant
BRC-BIO: Establishing Astrangia poculata as a study system to understand how multi-partner symbiotic interactions affect pathogen response in cnidarians
BRC-BIO:建立 Astrangia poculata 作为研究系统,以了解多伙伴共生相互作用如何影响刺胞动物的病原体反应
  • 批准号:
    2312555
  • 财政年份:
    2024
  • 资助金额:
    $ 16.16万
  • 项目类别:
    Standard Grant
RII Track-4:NSF: From the Ground Up to the Air Above Coastal Dunes: How Groundwater and Evaporation Affect the Mechanism of Wind Erosion
RII Track-4:NSF:从地面到沿海沙丘上方的空气:地下水和蒸发如何影响风蚀机制
  • 批准号:
    2327346
  • 财政年份:
    2024
  • 资助金额:
    $ 16.16万
  • 项目类别:
    Standard Grant
Graduating in Austerity: Do Welfare Cuts Affect the Career Path of University Students?
紧缩毕业:福利削减会影响大学生的职业道路吗?
  • 批准号:
    ES/Z502595/1
  • 财政年份:
    2024
  • 资助金额:
    $ 16.16万
  • 项目类别:
    Fellowship
Insecure lives and the policy disconnect: How multiple insecurities affect Levelling Up and what joined-up policy can do to help
不安全的生活和政策脱节:多种不安全因素如何影响升级以及联合政策可以提供哪些帮助
  • 批准号:
    ES/Z000149/1
  • 财政年份:
    2024
  • 资助金额:
    $ 16.16万
  • 项目类别:
    Research Grant
感性個人差指標 Affect-X の構築とビスポークAIサービスの基盤確立
建立个人敏感度指数 Affect-X 并为定制人工智能服务奠定基础
  • 批准号:
    23K24936
  • 财政年份:
    2024
  • 资助金额:
    $ 16.16万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
How does metal binding affect the function of proteins targeted by a devastating pathogen of cereal crops?
金属结合如何影响谷类作物毁灭性病原体靶向的蛋白质的功能?
  • 批准号:
    2901648
  • 财政年份:
    2024
  • 资助金额:
    $ 16.16万
  • 项目类别:
    Studentship
Investigating how double-negative T cells affect anti-leukemic and GvHD-inducing activities of conventional T cells
研究双阴性 T 细胞如何影响传统 T 细胞的抗白血病和 GvHD 诱导活性
  • 批准号:
    488039
  • 财政年份:
    2023
  • 资助金额:
    $ 16.16万
  • 项目类别:
    Operating Grants
New Tendencies of French Film Theory: Representation, Body, Affect
法国电影理论新动向:再现、身体、情感
  • 批准号:
    23K00129
  • 财政年份:
    2023
  • 资助金额:
    $ 16.16万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The Protruding Void: Mystical Affect in Samuel Beckett's Prose
突出的虚空:塞缪尔·贝克特散文中的神秘影响
  • 批准号:
    2883985
  • 财政年份:
    2023
  • 资助金额:
    $ 16.16万
  • 项目类别:
    Studentship
{{ showInfoDetail.title }}

作者:{{ showInfoDetail.author }}

知道了