Highly Multiplexed FISH for In Situ Genomics
用于原位基因组学的高度多重 FISH
基本信息
- 批准号:9065528
- 负责人:
- 金额:$ 24.51万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2015
- 资助国家:美国
- 起止时间:2015-05-08 至 2018-04-30
- 项目状态:已结题
- 来源:
- 关键词:AddressAlgorithmsAmendmentBar CodesBiological AssayBiopsyBiopsy SpecimenCancer DiagnosticsCancer cell lineCell NucleusCellsChromosome abnormalityChromosomesClinicClinicalClinical DataCommunity Clinical Oncology ProgramComputer softwareCopy Number PolymorphismCustomDNADNA ProbesDNA SequenceDataDetectionDevelopmentDiagnosticDiagnostic testsDimensionsERBB2 geneEpidermal Growth Factor ReceptorEvaluationEventFluorescence MicroscopyFluorescent in Situ HybridizationGene AmplificationGene DeletionGene DosageGene LibraryGenesGenetic HeterogeneityGenomicsGenotypeGlioblastomaGoldHealthHeterogeneityImageImage AnalysisIn SituInformaticsInstitutionKnowledgeLabelLaboratoriesLibrariesMalignant NeoplasmsMethodsMicroscopeMutationNeoplasm Circulating CellsNoiseNormal tissue morphologyOperative Surgical ProceduresOpticsPTEN genePatientsPerformancePopulation HeterogeneityRecurrenceResolutionSamplingSignal TransductionSlideSoftware ToolsSpecificitySpecimenSpottingsTechniquesTechnologyTestingTherapeuticTimeTissuesVariantcancer cellcell preparationcohortcombinatorialcomparative genomic hybridizationcomputerized data processingdeep sequencingdesigndiagnostic assaydigitaldigital imagingexperiencefluorophorefollow-upgenetic profilinggenome-wideimaging systemimprovedmicroscopic imagingmodel developmentneoplastic cellnext generation sequencingpredicting responsescreeningsequencing platformspectrographtargeted treatmenttechnology developmenttherapeutic targettreatment responsetumortumor DNAtumor heterogeneity
项目摘要
DESCRIPTION (provided by applicant): The overall objective of this application is to design and develop a technology permitting highly- multiplexed fluorescence in situ hybridization using probes from a broad library of genes. We will focus on genes whose copy number variation represents possible actionable therapeutic targets. We will build, test and validate an optimal assay platform leveraging our long-standing experience implementing diagnostic tests for chromosomal abnormalities in cancer. This objective will be achieved in two aims: Aim I. Develop a robust, reproducible assay for constructing a library of at least 50 locus-specific DNA sequence probes. We will use a combinatorial labeling approach in which each probe is bar-coded with a combination of two or three fluorophores per probe, allowing for up to 120 DNA probes to be simultaneously hybridized. We will develop an imaging system to decode the combinatorial label, record, quantify and analyze the obtained data. Aim II. We will begin to test the clinical utility of the assay by screening for actionable gene copy number alterations in surgical biopsy specimens and in isolated circulating tumor cells (CTCs). The development of this technology will allow us to get closer to address the question of whether patient-specific dynamics of tumor heterogeneity underlie variation in response to treatment and whether the evaluation of CTCs copy number profile in the follow up of treatment can predict response to therapy. This project will serve as a model for development and clinical implementation of diagnostics for the benefit of patients, and will be used to disseminate knowledge and expertise to the clinical cancer diagnostic field in general.
描述(由应用程序提供):本应用程序的总体目的是设计和开发一项技术,允许使用广泛基因库中的问题进行高度多重的荧光原位杂交。我们将专注于拷贝数变化代表可能可行的治疗靶标的基因。我们将建立,测试和验证一个最佳测定平台,以利用我们长期实施癌症异常诊断测试的经验。这一目标将在两个目标中实现:目标I。开发一种可重复的,可再现的测定法,以构建至少50个基因座特异性DNA序列问题的库。我们将使用一种组合标记方法,在该方法中,每个探针都用每个探针的两个或三个荧光团组合进行钢筋编码,从而使多达120个DNA问题简单地杂交。我们将开发一个成像系统来解码组合标签,记录,量化和分析所获得的数据。目标II。我们将开始通过筛选手术活检标本和分离的循环肿瘤细胞(CTC)中可起作的基因拷贝数改变来测试测定的临床实用性。这项技术的开发将使我们能够越来越近,以解决肿瘤异质性的特定特定动态的问题,这是对治疗的响应而变化的基础,以及对治疗后续的CTC拷贝数概况的评估是否可以预测治疗的反应。该项目将成为开发和临床实施诊断对患者益处的模型,并将用于将知识和专业知识传播到临床癌症诊断领域。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
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Anthony John Iafrate其他文献
Lynch syndrome screening in endometrial cancer patients with immunohistochemistry: A single center experience
- DOI:
10.1016/j.ygyno.2014.11.058 - 发表时间:
2015-02-01 - 期刊:
- 影响因子:
- 作者:
<u>Roberto</u> <u>Vargas</u>;J. Alejandro Rauh-Hain;Anthony John Iafrate;Daniel Chung;Leif Ellisen;Kristen Shannon;Linda Rodgers;Esther Oliva;John Schorge - 通讯作者:
John Schorge
High Throughput Microfluidics Platform to Assess Synthetic Lethality and Novel Therapeutic Drug Combinations
- DOI:
10.1182/blood-2023-190651 - 发表时间:
2023-11-02 - 期刊:
- 影响因子:
- 作者:
Anthony Soltis;Boryana Zhelyazkova;Pascal Drane;Efstathios Eleftheriadis;Andrew Ventresco;David Weitz;Anthony John Iafrate;Arlinda Lee - 通讯作者:
Arlinda Lee
Anthony John Iafrate的其他文献
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{{ truncateString('Anthony John Iafrate', 18)}}的其他基金
Highly Multiplexed FISH for In Situ Genomics
用于原位基因组学的高度多重 FISH
- 批准号:
9248273 - 财政年份:2015
- 资助金额:
$ 24.51万 - 项目类别:
Highly Multiplexed FISH for In Situ Genomics
用于原位基因组学的高度多重 FISH
- 批准号:
8810861 - 财政年份:2015
- 资助金额:
$ 24.51万 - 项目类别:
Predictive biomarker development in lung cancer: ROS1 chromosomal rearrangements
肺癌预测生物标志物的发展:ROS1染色体重排
- 批准号:
8166470 - 财政年份:2011
- 资助金额:
$ 24.51万 - 项目类别:
Predictive biomarker development in lung cancer: ROS1 chromosomal rearrangements
肺癌预测生物标志物的发展:ROS1染色体重排
- 批准号:
8298508 - 财政年份:2011
- 资助金额:
$ 24.51万 - 项目类别:
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