Maximizing small RNA delivery with signaling pepitdes

通过信号肽最大化小 RNA 递送

基本信息

  • 批准号:
    9145216
  • 负责人:
  • 金额:
    $ 23.59万
  • 依托单位:
  • 依托单位国家:
    美国
  • 项目类别:
  • 财政年份:
    2015
  • 资助国家:
    美国
  • 起止时间:
    2015-09-16 至 2018-06-30
  • 项目状态:
    已结题

项目摘要

 DESCRIPTION (provided by applicant): The objective of this mechanism-based research is to devise safe and efficient protein particulate nanocarriers (PPC) for small RNA delivery. To accomplish this, we plan to exploit the intracellular trafficking machinery to direct the delivery f small RNAs to their site of action while maximizing small RNA delivery. Small RNAs are used in a range of health applications. However, their potential has yet to be fully realized. This is largely due to inefficient delivery: only 1-2% of small RNAs reach the RNA-induced silencing complex (RISC): the site of action using conventional techniques. Small RNA delivery is a multistep process in which inefficiencies at any stage can compromise the efficacy of gene silencing. In particular, the intracellular fate of synthetic carriers is not well understood and tus poorly controlled. Recent studies indicate that active RISCs are functionally and physically coupled to MVBs and are not free in the cytoplasm as previously thought. Thus, we hypothesize that the effect of small RNA delivery can be significantly increased by actively targeting MVBs. We propose the design of PPCs containing signaling moieties that are recognized by the cell sorting machinery and that serve as "molecular zip codes" for the directed transport of small RNAs to the RISC. This could ultimately lead to enhanced gene silencing. This strategy could improve RNA-based therapy by allowing for the administration of lower doses of therapeutics, thereby reducing side effects and improving safety profiles.


项目成果

期刊论文数量(2)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(1)
The Phenotypic Effects of Exosomes Secreted from Distinct Cellular Sources: a Comparative Study Based on miRNA Composition.
  • DOI:
    10.1208/s12248-018-0227-4
  • 发表时间:
    2018-04-30
  • 期刊:
  • 影响因子:
    0
  • 作者:
    Ferguson S;Kim S;Lee C;Deci M;Nguyen J
  • 通讯作者:
    Nguyen J
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Juliane Nguyen其他文献

Juliane Nguyen的其他文献

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{{ truncateString('Juliane Nguyen', 18)}}的其他基金

Developing genetically encodable probes for multimodal tracking of exosomal RNA cargo
开发用于外泌体 RNA 货物多模式追踪的基因可编码探针
  • 批准号:
    10681827
  • 财政年份:
    2023
  • 资助金额:
    $ 23.59万
  • 项目类别:
Engineering a cross-linked cellular network for cardiac repair
设计用于心脏修复的交联细胞网络
  • 批准号:
    10539723
  • 财政年份:
    2022
  • 资助金额:
    $ 23.59万
  • 项目类别:
Polarizing Macrophages to Tumor Suppressors by Blocking Multiple CCR2 Chemokine Receptor Epitopes
通过阻断多个 CCR2 趋化因子受体表位将巨噬细胞极化为肿瘤抑制因子
  • 批准号:
    10380283
  • 财政年份:
    2021
  • 资助金额:
    $ 23.59万
  • 项目类别:
Polarizing Macrophages to Tumor Suppressors by Blocking Multiple CCR2 Chemokine Receptor Epitopes
通过阻断多个 CCR2 趋化因子受体表位将巨噬细胞极化为肿瘤抑制因子
  • 批准号:
    10328882
  • 财政年份:
    2020
  • 资助金额:
    $ 23.59万
  • 项目类别:
Polarizing Macrophages to Tumor Suppressors by Blocking Multiple CCR2 Chemokine Receptor Epitopes
通过阻断多个 CCR2 趋化因子受体表位将巨噬细胞极化为肿瘤抑制因子
  • 批准号:
    9973323
  • 财政年份:
    2020
  • 资助金额:
    $ 23.59万
  • 项目类别:
Polarizing Macrophages to Tumor Suppressors by Blocking Multiple CCR2 Chemokine Receptor Epitopes
通过阻断多个 CCR2 趋化因子受体表位将巨噬细胞极化为肿瘤抑制因子
  • 批准号:
    10737843
  • 财政年份:
    2020
  • 资助金额:
    $ 23.59万
  • 项目类别:
Polarizing Macrophages to Tumor Suppressors by Blocking Multiple CCR2 Chemokine Receptor Epitopes
通过阻断多个 CCR2 趋化因子受体表位将巨噬细胞极化为肿瘤抑制因子
  • 批准号:
    10524148
  • 财政年份:
    2020
  • 资助金额:
    $ 23.59万
  • 项目类别:
Polarizing Macrophages to Tumor Suppressors by Blocking Multiple CCR2 Chemokine Receptor Epitopes
通过阻断多个 CCR2 趋化因子受体表位将巨噬细胞极化为肿瘤抑制因子
  • 批准号:
    10559551
  • 财政年份:
    2020
  • 资助金额:
    $ 23.59万
  • 项目类别:
Self-replicating RNA-nanoplexes for programming monocytes to regenerate the heart
用于编程单核细胞以再生心脏的自我复制RNA纳米复合物
  • 批准号:
    8968584
  • 财政年份:
    2015
  • 资助金额:
    $ 23.59万
  • 项目类别:
Self-replicating RNA-nanoplexes for programming monocytes to regenerate the heart
用于编程单核细胞以再生心脏的自我复制RNA纳米复合物
  • 批准号:
    9105404
  • 财政年份:
    2015
  • 资助金额:
    $ 23.59万
  • 项目类别:

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