Tissue preservation tool for MDx

MDx 组织保存工具

• 批准号: 9141059
• 负责人: Jeanette R. Hill
• 金额: $ 22.5万
• 依托单位: SPOT ON SCIENCES, LLC
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-08-01 至 2017-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9141059
• 关键词: Archives; Area; Bar Codes; Biological Markers; Biological Preservation; Blood; Blood specimen; Buffers; Cancer Patient; Climacteric; Cold Chains; Collection; DNA; Data; Detection; Devices; Diagnostic tests; Disease; Ensure; Environment; Excision; Freezing; Goals; Immunochemistry; Label; Laboratories; Lead; Life; Location; Measures; Messenger RNA; Metabolic Diseases; Methods; MicroRNAs; Molecular; Molecular Analysis; Neonatal Screening; Nucleotides; Paper; Patients; Preparation; Procedures; Process; Proteins; Protocols documentation; Reagent; Recovery; Refrigeration; Research; Research Infrastructure; Resources; Reverse Transcriptase Polymerase Chain Reaction; Rural; Sampling; Science; Shipping; Ships; Solid; Source; Specimen; Spottings; Technology; Temperature; Testing; Therapeutic; TimeLine; Tissue Preservation; Tissue Sample; Tissues; analytical method; anticancer research; base; biobank; cancer diagnosis; cancer therapy; cost; cost effective; design; improved; innovation; meetings; molecular diagnostics; molecular marker; novel; personalized medicine; point of care; point-of-care diagnostics; predictive marker; prevent; prognostic assays; public health relevance; sample collection; small molecule; targeted treatment; tool

 DESCRIPTION (provided by applicant): Innovative tools for solid, tissue biospecimen preservation are needed to meet the promise of molecular diagnostics (MDx) and personalized treatment for cancer diagnoses and disease research. Spot On Sciences will develop an efficient and cost-effective method for solid biospecimen preservation that can be performed in any location while improving sample stability and accessibility for molecular analyses. For this method, a small sample is collected from a freshly excised tissue and quickly homogenized in stabilizing buffer. The homogenate is applied to absorbent paper within an enclosed cartridge and rapidly dried by a desiccant. Once dried, analytes including nucleotides, proteins and small molecules show stability at room temperature for years. Leveraging a novel blood collection device previously developed by Spot On Sciences, HemaSpot, the enclosed cartridge prevents sample contamination and allows facile shipment to a testing laboratory at ambient temperature with no cold chain requirements. The device is pre- labeled by bar code for simplified sample tracking. Based on well-established dried blood spot technology, common analytical methods such as RT- PCR, immunochemistry and LC-MS/MS are highly compatible with these dried samples. The pre- cut paper on the HemaSpot device allows for easy removal of sample subsections for molecular analyses and research. Preliminary data in our lab suggests feasibility for tissue to be preserved as a dried homogenate on filter paper. This study will further explore these results and demonstrate capability for measuring a wide range of analytes including nucleotides, proteins and small molecules from the same sample. The ultimate goal for this project is to provide a commercial kit for point of care (POC) diagnostic facilities containing all needed reagents and a simple device for collecting and storing homogenized tissue samples. Minimal infrastructure requirements will allow sample collection from any environment including remote, rural and low resource areas while providing stable, quality samples under a wide range of ambient conditions. This innovative method would greatly simplify and reduce costs for biospecimen collection and storage while increasing availability for high quality samples for research and for molecular testing for prognostic and therapeutic benefit for cancer patients.

 描述（由申请人提供）：需要用于固体组织生物标本保存的创新工具，以满足分子诊断（MDx）和癌症诊断和疾病研究的个性化治疗的承诺。Spot On Sciences将开发一种高效且具有成本效益的固体生物标本保存方法，该方法可以在任何地点进行，同时提高样品的稳定性和分子分析的可访问性。对于该方法，从新鲜切除的组织中收集少量样品，并在稳定缓冲液中快速均质化。将匀浆液涂于封闭药筒内的吸水纸上，并用干燥剂快速干燥。一旦干燥，包括核苷酸、蛋白质和小分子在内的分析物在室温下可稳定多年。利用Spot On Sciences之前开发的新型血液采集设备HemaSpot，封闭式检测盒可防止样本污染，并允许在环境温度下轻松运输到测试实验室，无需冷链要求。该设备是预先标记的条形码简化样品跟踪.基于成熟的干血斑技术，常规分析方法如RT-PCR、免疫化学和LC-MS/MS与这些干样品高度兼容。HemaSpot设备上的预切割纸允许轻松移除样品分段进行分子分析和研究。我们实验室的初步数据表明，将组织作为干燥匀浆保存在滤纸上是可行的。这项研究将进一步探索这些结果，并证明能够测量广泛的分析物，包括来自同一样品的核苷酸，蛋白质和小分子。该项目的最终目标是为护理点（POC）诊断设施提供商业试剂盒， 所需的试剂和用于收集和储存均质化组织样品的简单装置。最低限度的基础设施要求将允许从任何环境，包括偏远、农村和低资源地区采集样本，同时在各种环境条件下提供稳定、高质量的样本。这种创新的方法将大大简化和降低生物样本收集和储存的成本，同时增加高质量样本的可用性，用于研究和分子检测，为癌症患者提供预后和治疗益处。