(PQC2)High-content Pathology with Confocal Microscope Arrays
(PQC2)使用共焦显微镜阵列进行高内涵病理学
基本信息
- 批准号:8884395
- 负责人:
- 金额:$ 62.73万
- 依托单位:
- 依托单位国家:美国
- 项目类别:
- 财政年份:2013
- 资助国家:美国
- 起止时间:2013-09-01 至 2016-06-30
- 项目状态:已结题
- 来源:
- 关键词:AlgorithmsAppearanceArchitectureAreaAutomationBiological PreservationCancerousCellsCellular PhoneClinicalClinical ResearchComputer softwareCost SavingsDevelopmentDevicesDiagnosisDiagnosticEnsureEvaluationEventExcisionFeedbackFreezingFresh TissueFrozen SectionsFutureGoalsHandHead and Neck CancerHead and neck structureHealthcare SystemsHematoxylin and Eosin Staining MethodHistologicHistologyHistopathologyImageIndividualLinkMalignant NeoplasmsMechanicsMethodologyMethodsMicroscopeMicroscopicMicroscopyMiniaturizationModelingMolecularMolecular ProfilingNormal tissue morphologyOperative Surgical ProceduresOpticsPathologicPathologistPathologyPatientsPerformancePriceProceduresProcessQuality ControlReportingResectedResolutionSamplingSavingsScanningSeriesSpecificitySpecimenSpeedStagingStaining methodStainsSurfaceSurgeonSurgical PathologySystemTechniquesTechnologyTestingTimeTissue SampleTissuesUpdateVisualWritingbasebreast lumpectomycellular imagingcostdata acquisitiondesigndetectordigital imagingeffective interventionfluorophoreimage processingimage reconstructionimprovedinterestminiaturizeoperationpreventprospectiveprototypepublic health relevancescreeningsoftware developmenttissue processingtooltumor
项目摘要
DESCRIPTION (provided by applicant): Surgical removal of head and neck cancers presents challenges to the surgeon as they seek to remove cancerous tissue, preserve normal tissues and function, and prevent disfigurement. For this purpose, assessment of tissue status at the margins is critical for effective intervention. The pathologic examination of margins for tumors and the determination of "clean margins" is currently based on traditional frozen section methods that routinely examine less than 0.2% of the tissue removed. The aims of the proposed studies are to improve the assessment of tumor margins by developing tools that effectively move the microscopic examination closer to the patient in both time and space, and that provide improved guidance for tissue selection. This will be accomplished by developing, and deploying, arrays of miniaturized microscopes that reveal tissue histopathology at a resolution approaching that of conventional microscopes, and offer large coverage of tissue samples, and high speed scanning. These arrays serve as rapid screening tools, and are enabled by developments in integrated optics, and lithographic fabrication techniques such as MOEMS (micro-opto-electro-mechanical systems), and high- resolution, high-speed digital image capture and sophisticated image-processing algorithms to accelerate acquisition and refine quality of pathology images. We propose to use these arrays at the cutting bench in the surgical pathology suite to increase the amount of tissue analyzed in gross pathology, link macroscopic inspection of tissue to microscopic examination and provide more information, more rapidly, than conventional approaches. We will construct arrays of dual axis confocal (DAC) microscopes, advance rapid fluorescent surface staining, and develop image reconstruction software to enable rapid, microscopic inspection of large surface areas of resected tissue. The arrayed dual axis confocal (ADAC) device will be approximately the size of a cell phone and will provide cellular-level images of tissue architecture and molecular signatures at 1-5um resolution, and will be applied to the pathologic assessment of tumor margins. Although this study is limited to head and neck cancers, the full potential of ADAC is substantial. The margins of resection specimens are, in many cases, inadequately sampled due to the inefficiency of current sampling methods. The ADAC device could allow comprehensive en face examination of the entire lumpectomy specimen, and will have utility in selection of tissue for processing at the surgical pathology bench. As these tools advance, the histology lab could be circumvented for many specimens, which would provide a significant cost savings to the healthcare system, and increase the speed at which pathology could be reported.
描述(由申请人提供):头颈部癌症的外科切除对外科医生提出了挑战,因为他们寻求移除癌症组织,保护正常组织和功能,并防止毁容。为此,对边缘组织状态的评估是有效干预的关键。目前,肿瘤边缘的病理检查和“干净边缘”的确定是基于传统的冰冻切片方法,这种方法通常只检查不到0.2%的切除组织。拟议研究的目的是通过开发工具来改进对肿瘤边缘的评估,这些工具可以有效地在时间和空间上使显微镜检查更接近患者,并为组织选择提供更好的指导。这将通过开发和部署微型显微镜阵列来实现,这些阵列以接近传统显微镜的分辨率揭示组织病理,并提供大范围的组织样本覆盖和高速扫描。这些阵列用作快速筛选工具,并得益于集成光学和光刻制造技术的发展,如MOEMS(微光机电系统),以及高分辨率、高速数字图像捕获和复杂的图像处理算法,以加快病理图像的采集和提高质量。我们建议在外科病理学套件的切割工作台上使用这些阵列,以增加大体病理中分析的组织数量,将组织的宏观检查与显微检查联系起来,并比传统方法更快地提供更多信息。我们将构建双轴共焦(DAC)显微镜阵列,推进快速荧光表面染色,并开发图像重建软件,以实现对切除组织大表面积的快速显微检查。阵列式双轴共聚焦(ADAC)设备将大约有手机大小,并将以1-5微米的分辨率提供组织结构和分子签名的细胞级图像,并将应用于肿瘤边缘的病理评估。虽然这项研究仅限于头颈部癌症,但ADAC的全部潜力是巨大的。在许多情况下,由于目前的抽样方法效率低下,切除标本的边缘抽样不足。ADAC设备可以对整个肿块切除标本进行全面的正面检查,并将在外科病理工作台上选择组织进行处理。随着这些工具的进步,许多标本可以绕过组织学实验室,这将为医疗保健系统提供显著的成本节约,并提高报告病理的速度。
项目成果
期刊论文数量(0)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
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CHRISTOPHER H CONTAG其他文献
CHRISTOPHER H CONTAG的其他文献
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{{ truncateString('CHRISTOPHER H CONTAG', 18)}}的其他基金
9th Annual Meeting of the World Molecular Imaging Society - World Molecular Imaging Congress: "Imaging Biology... Improving Therapy"
世界分子影像学会第九届年会——世界分子影像大会:“影像生物学……改善治疗”
- 批准号:
9330483 - 财政年份:2016
- 资助金额:
$ 62.73万 - 项目类别:
Raman Molecular Imaging for Early Detection of Colon Cancer
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8819022 - 财政年份:2015
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$ 62.73万 - 项目类别:
Raman Molecular Imaging for Early Detection of Colon Cancer
拉曼分子成像用于结肠癌的早期检测
- 批准号:
9678998 - 财政年份:2015
- 资助金额:
$ 62.73万 - 项目类别:
(PQC2)High-content Pathology with Confocal Microscope Arrays
(PQC2)使用共焦显微镜阵列进行高内涵病理学
- 批准号:
8722518 - 财政年份:2013
- 资助金额:
$ 62.73万 - 项目类别:
Dynamic Imaging of EMT in the Breast Cancer Microenvironment
乳腺癌微环境中EMT的动态成像
- 批准号:
8577999 - 财政年份:2013
- 资助金额:
$ 62.73万 - 项目类别:
(PQC2)High-content Pathology with Confocal Microscope Arrays
(PQC2)使用共焦显微镜阵列进行高内涵病理学
- 批准号:
8591233 - 财政年份:2013
- 资助金额:
$ 62.73万 - 项目类别:
Dynamic Imaging for EMT in Breast Cancer Microenvironment
乳腺癌微环境中 EMT 的动态成像
- 批准号:
9690370 - 财政年份:2013
- 资助金额:
$ 62.73万 - 项目类别:
Dynamic Imaging of EMT in the Breast Cancer Microenvironment
乳腺癌微环境中EMT的动态成像
- 批准号:
8843268 - 财政年份:2013
- 资助金额:
$ 62.73万 - 项目类别:
Image-Guided Resection to Improve Outcome for Children with Medulloblastoma
图像引导切除术可改善髓母细胞瘤儿童的预后
- 批准号:
7990876 - 财政年份:2010
- 资助金额:
$ 62.73万 - 项目类别:
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