Building Enhanced Capability for the PennVet Multiphoton Core

为 PennVet 多光子核心构建增强功能

• 批准号: 9075603
• 负责人: BRUCE D FREEDMAN
• 金额: $ 60万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-05-01 至 2017-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9075603
• 关键词: Address; Applications Grants; Behavior; Brain; Calcium; Cells; Computer Systems; Core Facility; Data; Detection; Development; Fluorescence Resonance Energy Transfer; Funding; Future; Goals; Image; Immune response; In Situ; Infection; Intestines; Life; Lymphoid; Manuscripts; Microscope; Mitochondria; Organ; Pennsylvania; Schools; Signal Transduction; Signaling Molecule; Skin; Speed; Stream; System; Time; Tissues; Universities; Veterinary Medicine; base; flexibility; instrument; live cell imaging; pathogen; receptor; response; temporal measurement; tumor

 DESCRIPTION (provided by applicant): In response to the expanding need for advanced live cell imaging capabilities, the University of Pennsylvania School of Veterinary Medicine (SOVM) established an Imaging Core facility in the summer of 2008. With the involvement of several pioneer labs, and with nearly $900,000 contributed by the University Provosts office and Departments and Centers in the SOVM, we purchased several microscopes including a Leica SP5 multiphoton system. Expanding utilization of the core has been greatly facilitated by the expertise of our core director, Dr. Bruce Freedman and the core manager, currently Dr. Gordon Ruthel. Both have been instrumental in the development of a range of new applications for our users. As a group we have an enormous amount of collective expertise, including multiphoton real-time imaging of pathogen infection and immune responses in the brain, intestine, skin, and secondary lymphoid organs, as well as the behavior of tumors within the tissue matrix. FRET/FLIM based approaches are used to track the localization, interactions, and activity of signaling molecules in response to receptor stimulation, photo-activation to generate signaling intermediates instantaneously in situ, and live imaging of calcium and mitochondrial function. Since its inception, the data generated in this core has contributed to multiple manuscripts and successful grant applications. The experimental needs of our user group continuously drives us to expand our capability and each of the hardware components requested in this proposal was chosen to address specific current and future needs of our active user base. The ultimate goal of this application is to modernize this instrument and capitalize on technological developments to enhance multiphoton imaging capability that have become available since its installation. Funds requested will be used for hardware upgrades that will 1) increase the overall capability including depth of imaging, acquisition speed/temporal resolution, spatial coverage, detection sensitivity, and excitation flexibility 2) replace a legacy computer system that controls the instrument and add off line streaming and analysis capability, and 3) provide robust environmental control for long-term real time imaging of living tissues and cells.

 DESCRIPTION (provided by application): In response to the expanding need for advanced live cell imaging capabilities, the University of Pennsylvania School of Veterinary Medicine (SOVM) established an Imaging Core facility in the summer of 2008. With the involvement of several pioneer labs, and with nearly $900,000 contributed by the University Provosts office and Departments and Centers in the SOVM, we purchased several microscopes Expanding utilization of the core has我们的核心主任布鲁斯·弗里德曼（Bruce Freedman）博士和目前戈登·鲁瑟尔（Gordon Ruthel）博士的核心经理的专业知识得到了极大的支持。两者都在为我们的用户开发一系列新应用程序中发挥了重要作用。作为一个组，我们具有增强的集体专业知识，包括对病原体感染的多光子实时成像和大脑，肠道，皮肤和次级淋巴机器人器官中的免疫回应，以及组织基质内肿瘤的行为。基于FRET/FLIM的方法用于跟踪响应受体刺激的信号分子的本地化，相互作用和活性，光激活以产生信号传导即时地点中间，以及钙和线粒体功能的实时成像。自成立以来，该核心中生成的数据促成了多个手稿和成功的赠款应用程序。我们用户组的实验需求不断驱使我们扩大我们的功能，并且选择了本提案中要求的每个硬件组件来满足我们活跃用户群的特定当前和未来需求。该应用程序的最终目标是使该工具现代化并利用技术开发，以增强自安装以来已可用的多光子成像功能。所需的资金将用于硬件升级1）提高整体能力，包括成像的深度，获取速度/时间分辨率，空间覆盖范围，检测敏感性和兴奋性灵活性2）替换控制仪器的传统计算机系统，该系统可以控制乐器并添加离线流和分析能力，以及3）为长期的环境控制实用的现实时间表和实时的现实时间表，并提供了稳健的环境控制。

项目成果

Cysteine-rich domain of type III collagen N-propeptide inhibits fibroblast activation by attenuating TGFβ signaling.

• DOI: 10.1016/j.matbio.2022.03.004
• 发表时间: 2022-05
• 期刊: MATRIX BIOLOGY
• 影响因子: 6.9
• 作者: Brisson, Becky K.;Stewart, Daniel C.;Burgwin, Chelsea;Chenoweth, David;Wells, Rebecca G.;Adams, Sherrill L.;Volk, Susan W.
• 通讯作者: Volk, Susan W.

Static and time-dependent mechanical response of organic matrix of bone.

• DOI: 10.1016/j.jmbbm.2018.12.031
• 发表时间: 2019-03
• 期刊: Journal of the mechanical behavior of biomedical materials
• 影响因子: 3.9
• 作者: Saini K;Discher D;Kumar N
• 通讯作者: Kumar N

Piezo1 regulates the regenerative capacity of skeletal muscles via orchestration of stem cell morphological states.

• DOI: 10.1126/sciadv.abn0485
• 发表时间: 2022-03-18
• 期刊: Science advances
• 影响因子: 13.6
• 作者: Ma N;Chen D;Lee JH;Kuri P;Hernandez EB;Kocan J;Mahmood H;Tichy ED;Rompolas P;Mourkioti F
• 通讯作者: Mourkioti F

Platelet-rich plasma lysate displays antibiofilm properties and restores antimicrobial activity against synovial fluid biofilms in vitro.

• DOI: 10.1002/jor.24584
• 发表时间: 2020-06
• 期刊: Journal of orthopaedic research : official publication of the Orthopaedic Research Society
• 作者: Gilbertie JM;Schaer TP;Schubert AG;Jacob ME;Menegatti S;Ashton Lavoie R;Schnabel LV
• 通讯作者: Schnabel LV

The single mitochondrion of the kinetoplastid parasite Crithidia fasciculata is a dynamic network.

动质体寄生虫 Crithidia fasciculata 的单个线粒体是一个动态网络。

• DOI: 10.1371/journal.pone.0202711
• 发表时间: 2018
• 期刊: PloS one
• 影响因子: 3.7
• 作者: DiMaio,John;Ruthel,Gordon;Cannon,JoshuaJ;Malfara,MadelineF;Povelones,MeganL
• 通讯作者: Povelones,MeganL