Building Enhanced Capability for the PennVet Multiphoton Core

为 PennVet 多光子核心构建增强功能

• 批准号: 9075603
• 负责人: BRUCE D FREEDMAN
• 金额: $ 60万
• 依托单位: UNIVERSITY OF PENNSYLVANIA
• 依托单位国家: 美国
• 财政年份: 2016
• 资助国家: 美国
• 起止时间: 2016-05-01 至 2017-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9075603
• 关键词: Address; Applications Grants; Behavior; Brain; Calcium; Cells; Computer Systems; Core Facility; Data; Detection; Development; Fluorescence Resonance Energy Transfer; Funding; Future; Goals; Image; Immune response; In Situ; Infection; Intestines; Life; Lymphoid; Manuscripts; Microscope; Mitochondria; Organ; Pennsylvania; Schools; Signal Transduction; Signaling Molecule; Skin; Speed; Stream; System; Time; Tissues; Universities; Veterinary Medicine; base; flexibility; instrument; live cell imaging; pathogen; receptor; response; temporal measurement; tumor

 DESCRIPTION (provided by applicant): In response to the expanding need for advanced live cell imaging capabilities, the University of Pennsylvania School of Veterinary Medicine (SOVM) established an Imaging Core facility in the summer of 2008. With the involvement of several pioneer labs, and with nearly $900,000 contributed by the University Provosts office and Departments and Centers in the SOVM, we purchased several microscopes including a Leica SP5 multiphoton system. Expanding utilization of the core has been greatly facilitated by the expertise of our core director, Dr. Bruce Freedman and the core manager, currently Dr. Gordon Ruthel. Both have been instrumental in the development of a range of new applications for our users. As a group we have an enormous amount of collective expertise, including multiphoton real-time imaging of pathogen infection and immune responses in the brain, intestine, skin, and secondary lymphoid organs, as well as the behavior of tumors within the tissue matrix. FRET/FLIM based approaches are used to track the localization, interactions, and activity of signaling molecules in response to receptor stimulation, photo-activation to generate signaling intermediates instantaneously in situ, and live imaging of calcium and mitochondrial function. Since its inception, the data generated in this core has contributed to multiple manuscripts and successful grant applications. The experimental needs of our user group continuously drives us to expand our capability and each of the hardware components requested in this proposal was chosen to address specific current and future needs of our active user base. The ultimate goal of this application is to modernize this instrument and capitalize on technological developments to enhance multiphoton imaging capability that have become available since its installation. Funds requested will be used for hardware upgrades that will 1) increase the overall capability including depth of imaging, acquisition speed/temporal resolution, spatial coverage, detection sensitivity, and excitation flexibility 2) replace a legacy computer system that controls the instrument and add off line streaming and analysis capability, and 3) provide robust environmental control for long-term real time imaging of living tissues and cells.



项目成果

Cysteine-rich domain of type III collagen N-propeptide inhibits fibroblast activation by attenuating TGFβ signaling.

• DOI: 10.1016/j.matbio.2022.03.004
• 发表时间: 2022-05
• 期刊: MATRIX BIOLOGY
• 影响因子: 6.9
• 作者: Brisson, Becky K.;Stewart, Daniel C.;Burgwin, Chelsea;Chenoweth, David;Wells, Rebecca G.;Adams, Sherrill L.;Volk, Susan W.
• 通讯作者: Volk, Susan W.

Static and time-dependent mechanical response of organic matrix of bone.

• DOI: 10.1016/j.jmbbm.2018.12.031
• 发表时间: 2019-03
• 期刊: Journal of the mechanical behavior of biomedical materials
• 影响因子: 3.9
• 作者: Saini K;Discher D;Kumar N
• 通讯作者: Kumar N

Piezo1 regulates the regenerative capacity of skeletal muscles via orchestration of stem cell morphological states.

• DOI: 10.1126/sciadv.abn0485
• 发表时间: 2022-03-18
• 期刊: Science advances
• 影响因子: 13.6
• 作者: Ma N;Chen D;Lee JH;Kuri P;Hernandez EB;Kocan J;Mahmood H;Tichy ED;Rompolas P;Mourkioti F
• 通讯作者: Mourkioti F

The single mitochondrion of the kinetoplastid parasite Crithidia fasciculata is a dynamic network.

动质体寄生虫 Crithidia fasciculata 的单个线粒体是一个动态网络。

• DOI: 10.1371/journal.pone.0202711
• 发表时间: 2018
• 期刊: PloS one
• 影响因子: 3.7
• 作者: DiMaio,John;Ruthel,Gordon;Cannon,JoshuaJ;Malfara,MadelineF;Povelones,MeganL
• 通讯作者: Povelones,MeganL

Platelet-rich plasma lysate displays antibiofilm properties and restores antimicrobial activity against synovial fluid biofilms in vitro.

• DOI: 10.1002/jor.24584
• 发表时间: 2020-06
• 期刊: Journal of orthopaedic research : official publication of the Orthopaedic Research Society
• 作者: Gilbertie JM;Schaer TP;Schubert AG;Jacob ME;Menegatti S;Ashton Lavoie R;Schnabel LV
• 通讯作者: Schnabel LV