FLIM system, resonant scanner, and UV laser for 2 photon microscope

用于 2 光子显微镜的 FLIM 系统、共振扫描仪和紫外激光器

基本信息

批准号：
7794472
负责人：
BRUCE D FREEDMAN
金额：
$ 49.63万
依托单位：
UNIVERSITY OF PENNSYLVANIA
依托单位国家：
美国
项目类别：
财政年份：
2010
资助国家：
美国
起止时间：
2010-08-01 至 2011-07-31
项目状态：
已结题

项目摘要

DESCRIPTION (provided by applicant): In response to the expanding need for advanced live cell imaging capabilities, the School of Veterinary Medicine established an Imaging Core facility, which came online in the summer of 2008. With the heavy involvement of several pioneer labs, we have implemented a range of cutting edge applications and in less than one year have developed a dedicated and broad user base with 12 NIH funded labs actively generating data from the facility. In addition, 7 labs are in the consultation and experimental design stage, indicating a rapidly expanding interest in utilizing the core facility. Expanding utilization of the core has been greatly facilitated by the expertise of our core director, Dr. Lingli Zhang, who has been instrumental in developing and implementing a range of new applications for our user. As a group we have developed an enormous amount of collective expertise, including real-time imaging of pathogen infection and immune responses in the brain, intestine, skin, and secondary lymphoid organs, FRET based approaches to track the localization, interactions, and activity of signaling molecules in response to receptor stimulation, photo-activation to generate signaling intermediates instantaneously in situ, and live imaging of calcium and mitochondrial function. Although the core has been fully operational for a relatively short time, data generated have contributed to an Immunity paper, and results of other studies have been submitted for publication or are in final stages of preparation. The experimental needs of our user group continuously drives us to expand our capability and each of the hardware components requested in this proposal was chosen to address specific current needs, and also to anticipate future needs, with the ultimate goal of improving our 2 photon/spectral imaging capability. Funds requested will therefore be used for upgraded hardware that will enhance the capability of our core confocal/2 photon spectral imaging system in 3 general ways; 1) increase the temporal resolution, spatial coverage, and detection sensitivity of the microscope, 2) provide capability for drug application and adequate environmental control for long term real time imaging of living tissues and cells, and 3) expand the overall capability of the instrument to perform Forster resonance energy transfer (FRET) and fluorescence lifetime imaging.

描述（由申请人提供）：响应于对先进的活细胞成像功能的不断扩展的需求，兽医学院建立了一个成像核心设施，该设施于2008年夏天在线上进行。随着多个先锋实验室的大量参与，我们已经实施了一系列的最先进应用程序，并且在不到一年的时间内开发了一个专门的用户基础，并从事了一个专门的用户基础，该基础是12 nih fultity fultity fultity fute factity Intim falling fortiment factial inty Inih Ihih。此外，有7个实验室处于咨询和实验设计阶段，表明对利用核心设施的兴趣迅速扩大。我们的核心主管Lingli Zhang博士的专业知识极大地促进了核心的利用，他在为我们的用户开发和实施一系列新应用程序方面发挥了重要作用。 As a group we have developed an enormous amount of collective expertise, including real-time imaging of pathogen infection and immune responses in the brain, intestine, skin, and secondary lymphoid organs, FRET based approaches to track the localization, interactions, and activity of signaling molecules in response to receptor stimulation, photo-activation to generate signaling intermediates instantaneously in situ, and live imaging of calcium and线粒体功能。尽管核心在相对较短的时间内已经完全运行，但生成的数据促成了免疫论文，其他研究的结果已提交出版或处于准备的最后阶段。我们用户组的实验需求不断驱使我们扩大能力，并且选择了本提案中要求的每个硬件组件，以满足特定的当前需求，并预测未来的需求，以提高我们的2个光子/光谱成像能力。因此，所需的资金将用于升级的硬件，该硬件将通过3个一般方式来增强我们的核心共聚焦/2个光子光谱成像系统的能力； 1）增加显微镜的时间分辨率，空间覆盖范围和检测灵敏度，2）为药物施用和足够的环境控制提供了对生物组织和细胞的长期实时成像以及3）扩展仪器的整体能力以执行Forster Resonance Enermance Energy（FRET）（FRET）和荧光生存时间成像的能力。