Amygdala-Cerebellum Interactions During Associative Learning

 描述（由申请人提供）：许多神经系统疾病都存在记忆缺陷，记忆系统之间相互作用的破坏可能特别使人衰弱。不同的记忆系统通常会联合收割机并相互作用以影响日常行为。杏仁核和小脑传统上被认为是不同类型的记忆，情绪和运动记忆，分别是必不可少的球员。这些记忆系统在很大程度上被认为是独立的。然而，最近的证据表明，杏仁核促进小脑学习。我们的初步数据表明，大鼠杏仁核的可逆性失活严重损害了眨眼条件反射（一种小脑学习）的获得和保持。此外，杏仁核失活可逆地损害小脑中与学习相关的神经元活动的发展。本提案旨在阐明 杏仁核和小脑之间相互作用的潜在机制。目的1是通过杏仁核可逆性失活和小脑多电极神经元记录来确定杏仁核在眨眼条件反射中对小脑功能的影响。目的2是确定是否杏仁核与小脑相互作用，通过条件刺激输入途径小脑杏仁核的可逆失活，轴突追踪，电脑刺激，和多电极神经元记录。目的3是确定是否杏仁核记忆巩固是必要的，以促进小脑学习，在杏仁核蛋白质合成抑制和NMDA受体阻断和多电极神经元记录在脑桥核。这个项目将提供一个前所未有的杏仁核-小脑相互作用的分析。

 DESCRIPTION (provided by applicant): Memory deficits are found with many neurological disorders and the breakdown of interactions between memory systems can be particularly debilitating. Different memory systems typically combine and interact to influence everyday behavior. The amygdala and cerebellum have traditionally been viewed as essential players in different types of memory, emotional and motor memory, respectively. These memory systems have been viewed as largely independent. However, recent evidence suggests that the amygdala facilitates cerebellar learning. Our preliminary data indicate that reversible inactivatio of the amygdala in rats severely impairs acquisition and retention of eyeblink conditioning, a type of cerebellar learning. Moreover, amygdala inactivation reversibly impairs the development of learning-related neuronal activity in the cerebellum. This proposal is designed to elucidate the mechanisms underlying interactions between the amygdala and cerebellum. Aim 1 is to determine how the amygdala influences cerebellar function during eyeblink conditioning using reversible inactivation of the amygdala and multi-electrode neuronal recording in the cerebellum. Aim 2 is to determine whether or not the amygdala interacts with the cerebellum through the conditioned stimulus input pathway to the cerebellum using reversible inactivation of the amygdala, axonal tracing, electrical brain stimulation, and multi-electrode neuronal recording. Aim 3 is to determine whether or not amygdala memory consolidation is necessary for facilitating cerebellar learning using protein synthesis inhibition and NMDA receptor blockade in the amygdala and multi-electrode neuronal recording in the pontine nucleus. This project would provide an unprecedented analysis of amygdala-cerebellum interactions.