development of immunodeficient rabbit models
development of immunodeficient rabbit models
批准号:
9045309
负责人:
JIE XU
金额:
$18.62万
依托单位:
依托单位国家:
美国
项目类别:
财政年份:
2016
资助国家:
美国
项目状态:
已结题
起止时间:
2016-03-01 至 2017-08-31
关键词:
AllelesAllogenicAnimal ModelAnimal WelfareAnimalsBiomedical ResearchBusinessesCategoriesCell SurvivalCell TherapyCellsClustered Regularly Interspaced Short Palindromic RepeatsDNA cassetteDataDefectDevelopmentFamily suidaeGene TargetingGenesGenomeHairHealthHumanImmuneImmune responseImmunodeficient MouseImmunologic Deficiency SyndromesKnock-inKnock-outKnockout MiceLeadLongitudinal StudiesMediatingMethodsModelingMouse StrainsMusMutateNamesNude MiceOryctolagus cuniculusPhasePluripotent Stem CellsPre-Clinical ModelProceduresProductionProteinsRag1 MouseRegenerative MedicineReportingResearchResearch PersonnelRodentSafetySevere Combined ImmunodeficiencySmall Business Technology Transfer ResearchStem cellsSystemT-LymphocyteTechnologyTestingTetanus Helper PeptideTimeTissue GraftsTransplantationUnited StatesWorkXenograft procedureanimal efficacybasecancer stem cellclinically relevantcostfollow-uphuman diseasehuman stem cellsimprovedin vivointerestnonhuman primatephase 2 studypostnatalpre-clinicalpredict clinical outcomepublic health relevanceresponsesafety testingstem cell therapytool
中文摘要
英文摘要
DESCRIPTION (provided by applicant): Transplantation of human or animal pluripotent stem cells (PSCs) or their derivatives to model animals constitutes an important preclinical system to model and investigate cell survival, development and differentiation in vivo, and to assess the safety and efficacy of transplantation-based cell therapies. Immunodeficient mice are dominantly used in biomedical research as hosts for allogeneic and xenogeneic tissue grafts. In this STTR project, we propose to develop a large animal immunodeficient model, i.e. the rabbits. We propose to apply our expertise in CRISPR/Cas9 mediated gene targeting to knockout Foxn1, Rag2, Il2rg and Prkdc in rabbits. We also propose to test the feasibility of developing inducible immunodeficient rabbits. Rabbit is a useful model for regenerative medicine. It has been used to study a number of human diseases that can be treated with regenerative medicine. Immunodeficient rabbits, if successfully developed, can help develop procedures to assess the safety of stem cell therapies over clinically relevant time frames. They can be potentially important research tools to facilitate long-term follow-up studies of immune responses, xenotransplantation, stem cells, and cancer, thus constituting a remarkable preclinical model.
期刊论文(1)
专著(0)
科研奖励(0)
会议论文
DOI:
10.1016/j.stemcr.2021.01.010
发表时间:
2021-03-09
期刊:
Stem cell reports
影响因子:
5.9
作者:
[Song J, Hoenerhoff M, Yang D, Yang Y, Deng C, Wen L, Ma L, Pallas B, Zhao C, Koike Y, Koike T, Lester P, Yang B, Zhang J, Chen YE, Xu J]
通讯作者:
Xu J
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Derivation of Germline Competent Rabbit Embryonic Stem Cell Lines
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BIOELASTIC MATERIALS FOR DRUG ADDICTION INTERVENTION
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海外基金