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Skin Tissue Engineering

皮肤组织工程

基本信息

批准号：
9117969
负责人：
Bethany E Perez White
金额：
$ 25.35万
依托单位：
NORTHWESTERN UNIVERSITY AT CHICAGO
依托单位国家：
美国
项目类别：
财政年份：
2016
资助国家：
美国
起止时间：
2016-08-01 至 2019-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/9117969
关键词：
3-Dimensional Address Age Architecture Biology Biopsy Boxing Cataloging Catalogs Cell Communication Cell Culture System Cell Culture Techniques Cell Line Cell Lineage Cell Proliferation Cells Cellular Metabolic Process Clinical Research Coculture Techniques Collaborations Complex Cutaneous DNA Defect Dermal Dermatology Development Disease Embryo Engineering Environment Epidermis Equipment Ethnic Origin Experimental Designs Fibroblasts Gender Gene Delivery Gene Silencing Genetic Homeostasis Human Hypoxia Immigration Immunocompromised Host Institutional Review Boards Instruction Laboratories Libraries Longevity Maintenance Melanoma Cell Microscope Modeling Molecular Morphology Mus Names Neonatal Patients Phenotype Pre-Clinical Model Process Property Proteins RNA Race Reagent Research Research Personnel Services Site Skin Skin Tissue System Techniques Technology Testing Time Tissue Donors Tissue Engineering Tissues Training Ultraviolet Rays Universities Viral Virus Work base cell bank cell type cellular imaging cost cost effective drug discovery drug testing efficacy testing high throughput analysis humanized mouse improved in vivo induced pluripotent stem cell inhibitor/antagonist insight keratinocyte live cell imaging meetings melanocyte member mouse model novel therapeutics penis foreskin response scaffold sex skin disorder tissue culture tissue resource tool

项目摘要

Skin culture models serve as a key complementary tool for understanding normal and diseased skin biology by removing native cells from the complexity of the in vivo environment and into carefully controlled culture systems that facilitate mechanistic analysis. Primary skin cells can be expanded for high throughput analysis to provide a safe, pre-clinical model for drug discovery and test novel therapeutics. Isolated human cells can also be used to reconstruct 3-D skin equivalents that provide insight into mechanisms that govern tissue development, homeostasis and disease. The Skin Tissue Engineering Core is constructed to aid SDRC researchers in the use of primary skin culture models for their research. Towards this aim, the Core provides training, services and materials for the initiation and maintenance of primary skin cell cultures (i.e. keratinocytes, melanocytes and fibroblasts). Short- and long-term mouse keratinocyte cultures are generated for studies aimed at defining the cellular and molecular basis of skin defects evident in engineered mouse models. Users have access to a large supply of primary human keratinocytes isolated from neonatal foreskin but also an expanding library of control and patient keratinocytes from donors of various age, gender, race, body site, and disease. Viral, pharmacological and genetic reprogramming technologies are made available through the Core to overcome limitations in donor tissue availability. Training and provision of 3-D organotypic models of human epidermis established from control and patient skin cells allows investigators to address questions about skin biology in an architecturally appropriate manner where multiple cell types can interact with one another. The Core also provides skin culture reagents at reduced cost to users, access to specialized equipment amenable to skin biology studies, and integration with other SDRC (DNA/RNA Delivery; Morphology and Phenotyping) and institutional Cores (Cell Imaging Facility; High Throughput Analysis Laboratory; Human Embryonic and iPS Cell Facility) that enhances the utility of these skin culture models.

皮肤培养模型是了解正常和病变皮肤生物学的关键补充工具