Skin Tissue Engineering

皮肤培养模型是了解正常和疾病皮肤生物学的关键补充工具 通过将天然细胞从复杂的体内环境中移除并进入精心控制的培养中 便于机械分析的系统。原代皮肤细胞可进行扩增，以实现高通量分析 为药物发现和新疗法试验提供安全、临床前的模型。分离的人类细胞可以 也可用于重建3-D皮肤的等效物，从而深入了解控制组织的机制 发展、动态平衡和疾病。皮肤组织工程核心的构建是为了帮助国家发改委 研究人员使用初级皮肤培养模型进行他们的研究。为了实现这一目标，核心提供了 启动和维护原代皮肤细胞培养的培训、服务和材料(即 角质形成细胞、黑素细胞和成纤维细胞)。产生短期和长期的小鼠角质形成细胞培养 旨在确定基因工程小鼠明显皮肤缺陷的细胞和分子基础的研究 模特们。使用者可以获得大量从新生儿包皮中分离的原代人类角质形成细胞 而且还有一个不断扩大的对照和患者角质形成细胞库，这些细胞来自不同年龄，性别，种族， 身体部位和疾病。病毒、药理和基因重新编程技术已经可用 通过核心来克服捐赠者组织可获得性的限制。3-D的培训和提供 从对照和患者皮肤细胞建立的人类表皮器官模型允许研究人员 以架构上适当的方式解决有关皮肤生物学的问题，其中多种细胞类型 可以相互影响。Core还提供皮肤培养试剂，降低了用户的成本 适用于皮肤生物学研究的专门设备，并与其他SDRC(DNA/RNA)集成 交付；形态和表型)和机构核心(细胞成像设备；高吞吐量 分析实验室；人类胚胎和iPS细胞设施)，以增强这些皮肤培养的效用 模特们。

Skin culture models serve as a key complementary tool for understanding normal and diseased skin biology by removing native cells from the complexity of the in vivo environment and into carefully controlled culture systems that facilitate mechanistic analysis. Primary skin cells can be expanded for high throughput analysis to provide a safe, pre-clinical model for drug discovery and test novel therapeutics. Isolated human cells can also be used to reconstruct 3-D skin equivalents that provide insight into mechanisms that govern tissue development, homeostasis and disease. The Skin Tissue Engineering Core is constructed to aid SDRC researchers in the use of primary skin culture models for their research. Towards this aim, the Core provides training, services and materials for the initiation and maintenance of primary skin cell cultures (i.e. keratinocytes, melanocytes and fibroblasts). Short- and long-term mouse keratinocyte cultures are generated for studies aimed at defining the cellular and molecular basis of skin defects evident in engineered mouse models. Users have access to a large supply of primary human keratinocytes isolated from neonatal foreskin but also an expanding library of control and patient keratinocytes from donors of various age, gender, race, body site, and disease. Viral, pharmacological and genetic reprogramming technologies are made available through the Core to overcome limitations in donor tissue availability. Training and provision of 3-D organotypic models of human epidermis established from control and patient skin cells allows investigators to address questions about skin biology in an architecturally appropriate manner where multiple cell types can interact with one another. The Core also provides skin culture reagents at reduced cost to users, access to specialized equipment amenable to skin biology studies, and integration with other SDRC (DNA/RNA Delivery; Morphology and Phenotyping) and institutional Cores (Cell Imaging Facility; High Throughput Analysis Laboratory; Human Embryonic and iPS Cell Facility) that enhances the utility of these skin culture models.