Core B: Infectious Disease Assay Development

总结 传染病检测开发（IDAD）核心的总体目标是提供专业知识，设施， 服务，并在HTS检测设计，开发，验证，小型和大型领域的培训 筛选基于生物体（细胞）或生物化学的传染病靶标。检测方法开发和 对传染病靶标进行高通量筛选可以非常类似于其它生物学和 疾病目标;然而，通常存在与传染病目标相关的特定生物危害 可能需要特殊考虑和条件。这一核心将建立在现有资产的基础上， 通过核心主管和技术人员提供支持性专业知识和专注的关注， 传染病科学家及其生物学和疾病相关目标的社区。核心工作人员 将有助于设计、开发、验证适用于自动化高通量化学分析的测定方法， 筛选它们还将促进小规模或大规模的化合物筛选（在KU或从外部 来源）。这一核心和相关努力的预期成果是开发完善和经过验证的检测方法 适用于可在内部或外部进行的自动化大规模筛选。点击数 通过成功的检测开发和有限或大规模的筛选工作，将直接进入核心C 和核心D，以确定用作感染性疾病的分子探针和预治疗剂的先导化合物。 疾病这些专门知识和资源还将使调查人员能够制定有竞争力的提案， 对NIH资助机会的回应。

SUMMARY The overall goal of the Infectious Disease Assay Development (IDAD) Core is provide expertise, facilities, services, and training in the area of HTS assay design, development, validation, small and large-scale screening for organism (cell) based or biochemical infectious disease targets. Assay development and performing high-throughput screening for infectious disease targets can be very similar to other biological and disease targets; however, there are often specific biological hazards associated with infectious disease targets that can require special consideration and conditions. This core will build upon current assets and expand the current capabilities by providing enabling expertise and dedicated attention of a core director and a technician to the community of infectious disease scientists and their biologically and disease relevant targets. Core staff will facilitate design, development, validation of assays suitable for automated high-throughput chemical screening. They will also facilitate small or large scale screening of compounds (at KU or from external sources). The expected outcomes of this core and associated efforts are well developed and validated assays suitable for automated large-scale screening that can be performed internally or externally. Hits generated through successful assay development and limited or large scale screening efforts will be directed into Core C and Core D to identify lead compounds for use as molecular probes and pre-therapeutics for infectious diseases. The expertise and resources will also enable investigators to develop competitive proposals in response to NIH funding opportunities.