Interdepartmental Neuroscience Center

描述(由申请人提供)：这个神经科学中心核心设施，由Xandra Breakefield博士和Bakhos Tannous博士指导，将是一个制度上独特的资源，将把我们的神经科学教员聚集在一起，通过强大的、协同的新技术加强他们对神经学疾病的研究。提议的三个新核心是真正最先进的。成像核心(由布拉德·海曼博士和玛丽安·迪菲格里亚博士指导)将包括阵列断层扫描、透射式电子显微镜和体内详细大脑结构的多光子成像。微流体核心(由Daniel Irimia博士和Mehmet Toner博士指导)将提供专门的微流体设备和专业知识，用于细胞和细胞器分离的分析，以及高精度的细胞迁移和轴突引导的实时成像。载体核心(由Bakhos Tannous博士和Xandra Breakefield博士指导)将为所有项目生成各种病毒载体，并继续扩大其在该领域的进展，目前包括10种血清型的腺相关病毒(AAV)载体和外壳缺陷狂犬病病毒。监督这一计划的行政组织将包括P30主任/联席主任和核心主任/联席主任，以监测正在进行的活动并与用户互动；指导委员会，每年审查操作程序和活动报告，并就优先事项和核心有效性提供建议；咨询委员会，就技术更新和管理问题提供建议；以及行政核心，将通过专门的高级拨款经理为该计划提供后勤支持。所有这些服务将免费提供给NINDS资助的我们机构的神经科学研究人员，并将有助于创造一个非常有活力的互动环境，使想法成为现实。这群神经科学研究人员包括该领域的国际领军人物，以及初级和中级研究人员，对他们来说，神经系统障碍的研究是他们工作的主要重点，他们在该领域做出了许多重大贡献。我们的研究涵盖了由中风、损伤、脑肿瘤和癫痫引起的广泛的神经疾病，以及包括阿尔茨海默病、帕金森病和亨廷顿病在内的神经退行性疾病，以及儿童疾病，如早发性肌张力障碍、家族性自主神经障碍、遗传性感觉和自主神经疾病以及肾上腺脑白质营养不良。我们对这个PSO核心设施的愿景是，通过提供能够实现高分辨率大脑结构的技术，实现对疾病发病机制的更深层次的了解，并发现缓解方法。

DESCRIPTION (provided by applicant): This Neuroscience Center Core Facility, directed by Drs. Xandra Breakefield and Bakhos Tannous will be an institutionally unique resource which will bring our neuroscience faculty together and enhance their research on neurologic disorders through powerful, synergistic new technologies. The three new proposed cores are truly state-of-the-art. The Imaging Core (directed by Drs. Brad Hyman and Marian DiFiglia) will include array tomography, transmission electron microscopy and in vivo multiphoton imaging of detailed brain structure. The Microfluidics Core (directed by Drs. Daniel Irimia and Mehmet Toner) will provide specialized microfluidic devices and expertise in analysis for separation of cells and cell organelles, and high precision, real-time imaging of cell migration and axon guidance. The Vector Core (directed by Drs. Bakhos Tannous and Xandra Breakefield) will generate a variety of viral vectors for all projects and continue to expand its repertoire with advances in this field now including 10 serotypes of adeno-associated virus (AAV) vectors and coat-deficient rabies virus. The administration organization overseeing this program will include the P30 Director/Co-Director and Core Directors/Co-Directors to monitor ongoing activities and to interface with users; a Steering Committee to review operating procedures and activity reports on an annual basis, and to advise on prioritization issues and core effectiveness; an Advisory Board to offer advice on technology updates and management issues; and an Administrative Core which will logistically support the program with a dedicated senior grants manager All these services will be offered free-of-charge to the NINDS-funded neuroscience investigators at our institution and will serve to create a very dynamic, interactive environment where ideas become reality. This group of neuroscience investigators includes international leaders in the field, as well as junior and mid-level investigators for whom research on neurologic disorders is the main focus of their work and who have made many major contributions in the field. Our research covers a broad range of neurologic disorders due to stroke, injury, brain tumors and seizures, as well as neurodegenerative diseases including Alzheimer's disease, Parkinson's disease and Huntington's disease, and pediatric disorders, such as early onset dystonia, familial dysautonomia, hereditary sensory and autonomic neuropathy and adrenoleukodystrophy. Our vision for this PSO Core Facility is to achieve a deeper level of understanding of disease pathogenesis and to discover means of alleviation by providing technologies which can achieve high resolution of brain structure.