TECHNOLOGY NMR SPECTROSCOPY

核磁共振波谱技术

• 批准号: 9139468
• 负责人: K Sreekumaran Nair
• 金额: $ 27.73万
• 依托单位: MAYO CLINIC ROCHESTER
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/9139468
• 关键词: Aging; Animals; Area; Biochemical Pathway; Bioinformatics; Biological Markers; Blood specimen; Body Fluids; Brain; Carbon; Cardiovascular system; Cerebrospinal Fluid; Clinic; Clinical; Core Facility; Cost Effectiveness Analysis; Data; Data Analyses; Data Display; Deposition; Diabetes Mellitus; Diagnostic; Ensure; Exercise; Goals; Heart; Human; Image Analysis; Institution; Instruction; Kidney; Label; Liver; Malignant Neoplasms; Mass Spectrum Analysis; Measurement; Metabolic; Methods; Monitor; Muscle; NMR Spectroscopy; Neurodegenerative Disorders; Nuclear Magnetic Resonance; Organ; Pathway interactions; Patient Monitoring; Plasma; Procedures; Process; Protocols documentation; Quality Control; Regenerative Medicine; Research Personnel; Resolution; Resources; Sampling; Series; Services; Specialized Center; Spectrum Analysis; Stress; Technology; Testing; Tissue Extracts; Tissues; Tracer; Training; Translating; United States National Institutes of Health; Urine; Whole Blood; Work; advanced disease; base; biomarker panel; data acquisition; data sharing; disease diagnosis; drug development; experience; high standard; high throughput screening; human disease; human tissue; improved; in vivo; individualized medicine; instrument; instrumentation; magnetic resonance spectroscopic imaging; metabolomics; metabolomics resource; novel; outcome forecast; outreach; personalized medicine; programs; research to practice; stable isotope; tool; treatment strategy

As an integral part of Mayo Clinic Comprehensive Metabolomics Resource Core (MCCMRC) the NMR Metabolomics core provides targeted analysis of metabolomic profiles using Nuclear Magnetic Resonance (NMR) Spectroscopy. In addition to establishing standard NMR high-throughput 1H and 31P NMR metabolomics analyses, the core will implement, its unique methods of 18O labeling and 18O-assisted 31P NMR spectroscopy for studies of phosphometabolite dynamics and 13C NMR for carbon isotopomer exchange in metabolic networks, a procedure that is integrated in Mass Spectrometry core. This integrated approach of qualitative and quantitative analysis of tissue extracts (1H and 31P NMR), stable isotope tracing (13C and 18O-assisted 31P NMR spectroscopy) and in vivo spectroscopy (1H, 31P volume selective NMR spectroscopy, NMR spectroscopic imaging) will include the analysis of human and animal samples (e.g., whole blood, plasma, cerebrospinal fluid, urine, heart, liver, brain, kidney) and in vivo intact organs (brain, heart, kidney, muscle). In addition, we will implement protocols for stress metabolomic testing before and after exercise. The NMR Metabolomics core is supported by Analytical NMR Facility that has 5 state-of-theart 300 MHz, 500 MHz, 600 MHz, 700 MHz and wide bore 700 MHz NMR spectrometers and is staffed with experienced NMR spectroscopists. We propose the acquisition of a high sensitivity 600 MHz BB cryoprobe, high-throughput sample handler and additional technical staff to expand the core capacity and analytical capabilities at least threefold for 1H and 4-9 fold for 31P and 13C NMR (from -25 to 100 samples/day) and to offer services and to a larger number of investigators inside and outside Mayo Clinic. The core will implement standardized metabolomic protocols and methods of NMR data acquisition, processing, metabolite identification, bioinformatic pathway and flux analysis and interpretation. The core will develop appropriate workflow that will ensure cost-effective analysis of series of samples, and quality control. The core staff will work with the MCCMRC Management to implement consistent formats to deposit raw, processed and analyzed data according to the data sharing plan. The NMR Metabolomic core currently assists over 20 NIH supported investigators in cardiovascular, aging, diabetes, cancer and neurodegenerative disease, and regenerative and individualized medicine areas. We plan to make the program self-sustaining in five years. Thus the overall goal of this application is to consolidate and expand the NMR Metabolomic core facility and services it provides and to offer our expertise to other national institutions serving as a regional hub to advance stable isotope and NMR based translational and clinical metabolomics.

作为 Mayo Clinic 综合代谢组学资源核心 (MCCMRC) 的组成部分，NMR 代谢组学核心使用核磁共振提供代谢组谱的靶向分析 （核磁共振）光谱学。除了建立标准 NMR 之外，还建立了高通量 1H 和 31P NMR 代谢组学分析，核心将实施其独特的18O标记和18O辅助31P方法 用于研究磷酸代谢物动力学的 NMR 波谱和用于研究碳同位素异构体的 13C NMR 代谢网络中的交换，这是集成在质谱核心中的过程。这个集成的 组织提取物的定性和定量分析方法（1H 和 31P NMR）、稳定同位素示踪 （13C 和 18O 辅助 31P NMR 光谱）和体内光谱（1H、31P 体积选择性 NMR 光谱、核磁共振光谱成像）将包括人类和动物样本的分析（例如， 全血、血浆、脑脊液、尿液、心脏、肝脏、脑、肾）和体内完整器官（脑、 心脏、肾脏、肌肉）。此外，我们将在之前和之后实施压力代谢组测试方案。 运动后。 NMR 代谢组学核心由分析 NMR 设施提供支持，该设施拥有 5 个最先进的 300 MHz、500 MHz、600 MHz、700 MHz 和宽口径 700 MHz NMR 波谱仪，并配备 经验丰富的核磁共振波谱学家。我们建议购买高灵敏度 600 MHz BB 冷冻探针， 高通量样品处理程序和额外的技术人员，以扩大核心能力和分析能力 1H 的能力至少是原来的三倍，31P 和 13C NMR 的能力至少是 4-9 倍（从 -25 到 100 个样本/天） 为梅奥诊所内外的大量研究人员提供服务。核心将 实施标准化代谢组协议和 NMR 数据采集、处理、 代谢物鉴定、生物信息途径以及通量分析和解释。核心将发展 适当的工作流程将确保对一系列样品进行经济高效的分析和质量控制。这 核心工作人员将与 MCCMRC 管理层合作，实施一致的格式来存入原始、 根据数据共享计划对数据进行处理和分析。目前 NMR 代谢组学核心 协助 20 多名 NIH 支持的心血管、衰老、糖尿病、癌症和神经退行性疾病研究人员 疾病、再生和个体化医学领域。我们计划制作这个节目 五年内实现自给自足。因此，该应用程序的总体目标是巩固和扩展 NMR 它提供代谢组学核心设施和服务，并向其他国家机构提供我们的专业知识 作为推进基于稳定同位素和核磁共振的转化和临床代谢组学的区域中心。