A web-based craniofacial disease gene discovery tool

基于网络的颅面疾病基因发现工具

• 批准号: 9107846
• 负责人: Salil Lachke
• 金额: $ 19.25万
• 依托单位: UNIVERSITY OF DELAWARE
• 依托单位国家: 美国
• 财政年份: 2015
• 资助国家: 美国
• 起止时间: 2015-08-01 至 2018-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/9107846
• 关键词: Address; Affect; Bioinformatics; Candidate Disease Gene; Clinical; Communities; Complex; Computer Simulation; Congenital Abnormality; Craniofacial Abnormalities; Data; Data Set; Defect; Development; Disease; Effectiveness; Embryo; Event; Exhibits; Eye; Eye Development; Eye diseases; Face; FaceBase; Future; Gene Expression; Genes; Genetic Predisposition to Disease; Genomics; Health; Histocompatibility Testing; Human; In Situ Hybridization; Individual; Knowledge; Letters; Literature; Live Birth; Machine Learning; Medical; Methods; Molecular; Molecular Profiling; Movement; Mus; Online Systems; Pathogenesis; Patients; Persons; Play; Process; Productivity; Publishing; Regulator Genes; Research; Resources; Scientist; Series; Staging; Structural Congenital Anomalies; System; Tissues; United States National Institutes of Health; Visual Fields; base; cell type; cost effective; craniofacial; craniofacial development; data mining; evidence base; exome sequencing; gene discovery; gene interaction; genome browser; genome wide association study; genome-wide; improved; innovation; interactive tool; interest; learning strategy; lens; life time cost; microdeletion; novel; novel strategies; novel therapeutic intervention; orofacial cleft; rehabilitation service; success; tool; transcriptome sequencing; user-friendly; web based interface; whole genome

 DESCRIPTION (provided by applicant): Craniofacial (CF) abnormalities constitute more than a third of all human structural birth defects. To define their genetic etiology, detailed molecular understanding is required of coordinated movement and fusion of embryonic facial prominences - as disruption of these morphogenetic events cause defects such as orofacial clefts (OFC). The NIH FaceBase initiative is an important step to address this need, as it aims to generate comprehensive whole-genome expression datasets using microarrays or Next-Gen RNA-sequencing (RNA-seq) on mouse embryonic CF tissue. However, genome-wide profiling identifies several thousand "expressed" genes and it is a formidable challenge to predict and prioritize the select few genes that are critical to tissue development or pathogenesis. We posit that although there is a wealth of genomic-level data available, this deficit remains because an adequate strategy has not yet been applied to identify these important candidate CF genes. We recently developed an innovative approach - termed in silico whole embryo body (WB) subtraction - to identify such important genes based on developmentally-enriched expression. We have applied this novel approach to ~15% of FaceBase data and assembled this knowledge as a user-friendly web-based interactive tool SysFACE (Systems tool for craniofacial expression-based gene discovery, http://bioinformatics.udel.edu/Research/SysFACE). Even with limited datasets, the beta version of SysFACE is significantly more effective, compared with unprocessed FaceBase datasets, in identification of known genes associated with OFCs from both linkage and GWAS studies. To process all existing FaceBase datasets, we will generate additional platform-specific WB reference datasets and evaluate these further with machine learning strategies to identify genes important to CF development (Aim 1). Subsequently, we aim to experimentally validate these tissue-enriched gene expression profiles, and to assemble this knowledge - along with a new evidence-based functional gene regulatory network (GRN) that will allow all molecular data from the CF published literature to be represented on systems level - as a user-friendly web-based interactive resource (Aim 2), which will also be made available through FaceBase. Development of SysFACE, as outlined in this application, will greatly improve prediction of candidate CF genes, provide an excellent resource for CF-network construction, and will facilitate CF gene discovery efforts by developmental biologists and clinicians.

 描述(由申请人提供)：颅面(CF)异常占人类所有结构性出生缺陷的三分之一以上。为了确定他们的遗传病因，详细的分子 胚胎面部隆起的协调运动和融合需要理解--因为这些形态发生事件的中断会导致口面部裂隙(OFC)等缺陷。NIH FaceBase计划是满足这一需求的重要一步，因为它的目标是使用微阵列或下一代RNA测序(RNA-seq)在小鼠胚胎CF组织上生成全面的全基因组表达数据集。然而，全基因组图谱识别了数千个“表达的”基因，预测和优先选择对组织发育或致病关键的少数基因是一个艰巨的挑战。我们假设，尽管有大量的基因组水平的数据可用，但这种缺陷仍然存在，因为还没有足够的策略来识别这些重要的候选CF基因。我们最近开发了一种创新的方法-被称为电子整体胚体(WB)减法-基于发育丰富的表达来识别这些重要的基因。我们已经将这种新的方法应用于约15%的FaceBase数据，并将这些知识整合为一个用户友好的基于网络的交互工具SysFACE(基于颅面表情的基因发现系统工具，http://bioinformatics.udel.edu/Research/SysFACE).即使在数据集有限的情况下，与未经处理的FaceBase数据集相比，SysFACE的测试版在从连锁研究和GWAS研究中识别与OFCs相关的已知基因方面明显更有效。为了处理所有现有的FaceBase数据集，我们将生成额外的特定平台的WB参考数据集，并使用机器学习策略进一步评估这些数据集，以确定对CF开发重要的基因(目标1)。随后，我们的目标是在实验上验证这些组织丰富的基因表达谱，并将这些知识与一个新的基于证据的功能基因调控网络(GRN)结合在一起，作为一个用户友好的基于网络的交互资源(AIM 2)，该资源也将通过FaceBase提供。本申请概述的SysFACE的开发将极大地提高对候选CF基因的预测，为CF网络的构建提供良好的资源，并将促进发育生物学家和临床医生的CF基因发现工作。